Cysteine Dotaz Zobrazit nápovědu
2nd ed. xxxv, 1051-2140, [90] s. : il. + 1 optický disk
- MeSH
- aplikace lokální MeSH
- cystein MeSH
- králíci MeSH
- lékové formy MeSH
- radioprotektivní látky MeSH
- Check Tag
- králíci MeSH
Cysteinové proteasy se zásadním způsobem účastní mnoha fyziologických a patologických procesů v těle člověka. Jejich dysregulace vede k rozvoji mnoha těžkých onemocnění, jako jsou např. ateroskleróza, osteoporóza, revmatoidní artritida, nealkoholická steatohepati-tida, neurodegenerativní a nádorová onemocnění. Vzhledem ke své roli by se cysteinové proteasy mohly stát atraktivním cílem, pro návrh nových typů léčiv. Tento přehledný článek se zaměřuje na cysteinové proteasy z klanů CA a CD a shrnuje současné poznatky o jejich roli v patologii člověka.
Cysteine proteases are critically involved in numerous physiological and pathological processes in the human body. Their deregulation is associated with severe pathologies, such as athersosclerosis, osteoporosis, rheumatoid arthritis, nonalcoholic steatohepatitis, neurode-generative and neoplastic diseases. Therefore, cysteine proteases are attractive target molecules for rational design of novel types of che-motherapeutics. This review provides current knowledge on cysteine proteases from clans CA and CD and their role in human pathology.
- MeSH
- Alzheimerova nemoc diagnóza etiologie farmakoterapie MeSH
- ateroskleróza diagnóza etiologie farmakoterapie MeSH
- cysteinové proteasy * chemie klasifikace škodlivé účinky MeSH
- inhibitory cysteinových proteinas terapeutické užití MeSH
- lidé MeSH
- nádory etiologie MeSH
- nealkoholová steatóza jater diagnóza etiologie farmakoterapie MeSH
- osteoporóza diagnóza etiologie farmakoterapie MeSH
- revmatoidní artritida diagnóza etiologie farmakoterapie MeSH
- rizikové faktory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
- MeSH
- fluorescenční mikroskopie MeSH
- lidé MeSH
- nádory plic analýza MeSH
- proteasy MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
Silver solid amalgam electrode (AgSAE) was used for differential pulse voltammetric (DPV) measurements of cysteine and cysteine-containing peptides, glutathione, gamma-Glu-Cys-Gly and phytochelatin (gamma-Glu-Cys)(3)-Gly (PC3), in the presence of Co(II) ions. It had been established earlier that cysteine-containing peptides and proteins catalyze hydrogen evolution at mercury electrodes in presence of cobalt salts; these processes are known as the Brdicka reaction. DPV signals measured with the AgSAE, the surfaces of which had been modified by mercury meniscus or mercury film, were qualitatively the same as those obtained with the hanging mercury drop electrode (HMDE). With these electrodes the number and the intensity of Brdicka signals of cysteine, glutathione and PC3 differed, making a distinction among them possible. On the other hand, with the polished silver solid amalgam electrode (the surface of which was completely free of liquid mercury) all three compounds produced only one but strikingly intense peak in the region of Brdicka reaction. Using this signal, cysteine, glutathione as well as PC3 could be determined at 10(-8)M level, representing sensitivity up to 2 orders of magnitude better than attained with the mercury-modified AgSAEs or HMDE.
Cysteine proteases have been discovered in various bloodfeeding ectoparasites. Here, we assemble the available information about the function of these peptidases and reveal their role in hematophagy and parasite development. While most of the data shed light on key proteolytic events that play a role in arthropod physiology, we also report on the association of cysteine proteases with arthropod vectorial capacity. With emphasis on ticks, specifically Ixodes ricinus, we finally propose a model about the contribution of cysteine peptidases to blood digestion and how their concerted action with other tick midgut proteases leads to the absorbance of nutrients by the midgut epithelial cells.
- MeSH
- členovci enzymologie MeSH
- Culicidae enzymologie MeSH
- cysteinové proteasy metabolismus MeSH
- klíšťata enzymologie MeSH
- paraziti enzymologie MeSH
- stravovací zvyklosti fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
