PURPOSE: This study investigated the effects of acute, pre-exercise, hydrogen rich water (HRW) ingestion on running time to exhaustion at maximal aerobic speed in trained track and field runners. METHODS: Twenty-four, male runners aged 17.5 ± 1.8 years, with body mass index = 21.0 ± 1.3 kg⋅m-2, and maximal oxygen uptake = 55.0 ± 4.6 ml⋅kg-1⋅min-1 (mean ± standard deviation) participated in this randomized, double-blind, placebo-controlled crossover study. All runners ingested 1260 ml of HRW which was divided into four doses and taken at 120 min (420 ml), 60 min (420 ml), 30 min (210 ml), and 10 min (210 ml) prior to exercise. The running protocol consisted of three phases: warm-up performed at 10 km⋅h-1 for 3 min, followed by a transition phase performed at an individually determined speed (10 km⋅h-1 + maximal aerobic speed)/2 for 1 min, and finally the third phase performed at individual maximal aerobic speed until exhaustion. Time to exhaustion, cardiorespiratory variables, and post-exercise blood lactate concentration were measured. RESULTS: When running to exhaustion at maximal aerobic speed, compared with placebo, HRW had no significant effects on the following variables: time to exhaustion (217 ± 49 and 227 ± 53 s, p = 0.20), post-exercise blood lactate concentration (9.9 ± 2.2 and 10.1 ± 2.0 mmol⋅L-1, p = 0.42), maximal heart rate (186 ± 9 and 186 ± 9 beats⋅min-1, p = 0.80), and oxygen uptake (53.1 ± 4.5 and 52.2 ± 4.7 ml⋅kg-1⋅min-1, p = 0.33). No variable assessed as a candidate moderator was significantly correlated with time to exhaustion (Spearman's correlation coefficients ranged from -0.28 to 0.30, all p ≥ 0.16). CONCLUSIONS: Pre-exercise administration of 1260 ml of HRW showed no ergogenic effect on running performance to exhaustion at maximal aerobic speed in trained track and field runners.

Department of Medical Biophysics Faculty of Medicine and Dentistry Palacký University Olomouc Olomouc Czech Republic

Department of Natural Sciences in Kinanthropology Faculty of Physical Culture Palacký University Olomouc Olomouc Czech Republic

Discipline of Biokinetics Exercise and Leisure Sciences School of Health Sciences University of KwaZulu Natal Durban South Africa

Institute of Molecular and Translational Medicine Faculty of Medicine and Dentistry Palacký University Olomouc Olomouc Czech Republic

Research Institute for Sport and Exercise University of Canberra Bruce Australia

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Dole M, Wilson FR, Fife WP. Hyperbaric hydrogen therapy: A possible treatment for cancer. Science. 1975;190(4210):152–4. doi: 10.1126/science.1166304 PubMed DOI

Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, et al.. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 2007;13(6):688–94. doi: 10.1038/nm1577 PubMed DOI

Ichihara M, Sobue S, Ito M, Ito M, Hirayama M, Ohno K. Beneficial biological effects and the underlying mechanisms of molecular hydrogen—comprehensive review of 321 original articles. Med Gas Res. 2015;5(12):1–21. doi: 10.1186/s13618-015-0035-1 PubMed DOI PMC

Aoki K, Nakao A, Adachi T, Matsui Y, Miyakawa S. Pilot study: Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes. Med Gas Res. 2012;2(12):1–6. doi: 10.1186/2045-9912-2-12 PubMed DOI PMC

Da Ponte A, Giovanelli N, Nigris D, Lazzer S. Effects of hydrogen rich water on prolonged intermittent exercise. J Sports Med Phys Fitness. 2018;58(5):612–21. doi: 10.23736/S0022-4707.17.06883-9 PubMed DOI

Botek M, Khanna D, Krejčí J, Valenta M, McKune A, Sládečková B, et al.. Molecular hydrogen mitigates performance decrement during repeated sprints in professional soccer players. Nutrients. 2022;14(508):1–10. doi: 10.3390/nu14030508 PubMed DOI PMC

Timón R, Olcina G, González-Custodio A, Camacho-Cardenosa M, Camacho-Cardenosa A, Martínez Guardado I. Effects of 7-day intake of hydrogen-rich water on physical performance of trained and untrained subjects. Biol Sport. 2021;38(2):269–75. doi: 10.5114/biolsport.2020.98625 PubMed DOI PMC

Botek M, Krejčí J, McKune A, Valenta M, Sládečková B. Hydrogen rich water consumption positively affects muscle performance, lactate response, and alleviates delayed onset of muscle soreness after resistance rraining. J strength Cond Res. 2021;1–8. PubMed

Botek M, Krejčí J, McKune AJ, Sládečková B. Hydrogen-rich water supplementation and up-hill running performance: Effect of athlete performance level. Int J Sports Physiol Perform. 2020;15(8):1193–6. doi: 10.1123/ijspp.2019-0507 PubMed DOI

Botek M, Krejčí J, McKune AJ, Sládečková B, Naumovski N. Hydrogen rich water improved ventilatory, perceptual and lactate responses to exercise. Int J Sports Med. 2019;40(14):879–85. doi: 10.1055/a-0991-0268 PubMed DOI

Hong Y, Dong G, Li Q, Wang V, Liu M, Jiang G, et al.. Effects of pre-exercise H2 inhalation on physical fatigue and related prefrontal cortex activation during and after high-intensity exercise. Front Physiol. 2022;13(988028):1–10. PubMed PMC

Ostojić SM, Stojanović MD, Calleja-Gonzalez J, Obrenović MD, Veljović D, Medjedović B, et al.. Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women: Double-blind, randomized, placebo-controlled, corss-over trial of efficacy and safety. Serbian J Sport Sci. 2011;5(3):83–9.

Kawamura T, Muraoka I. Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint. Antioxidants. 2018;7(9):1–19. doi: 10.3390/antiox7090119 PubMed DOI PMC

Ooi CH, Ng SK, Omar EA. Acute ingestion of hydrogen-rich water does not improve incremental treadmill running performance in endurance-trained athletes. Appl Physiol Nutr Metab. 2020;45(5):513–9. doi: 10.1139/apnm-2019-0553 PubMed DOI

Ito H, Kabayma S, Goto K. Effects of electrolyzed hydrogen water ingestion during endurance exercise in a heated environment on body fluid balance and exercise performance. Temperature. 2020;7(3):290–9. PubMed PMC

Murakami Y, Ito M, Ohsawa I. Molecular hydrogen protects against oxidative stress-induced SH-SY5Y neuroblastoma cell death through the process of mitohormesis. PLoS One. 2017;12(5):1–14. doi: 10.1371/journal.pone.0176992 PubMed DOI PMC

Gvozdjáková A, Kucharská J, Kura B, Vančová O, Rausová Z, Sumbalová Z, et al.. A new insight into the molecular hydrogen effect on coenzyme Q and mitochondrial function of rats. Can J Physiol Pharmacol. 2020;98(1):29–34. doi: 10.1139/cjpp-2019-0281 PubMed DOI

Lacour JR, Padilla-Magunacelaya S, Barthélémy JC, Dormois D. The energetics of middle-distance running. Eur J Appl Physiol Occup Physiol. 1990;60(1):38–43. doi: 10.1007/BF00572183 PubMed DOI

Kang M, Ragan BG, Park JH. Issues in outcomes research: An overview of randomization techniques for clinical trials. J Athl Train. 2008;43(2):215–21. doi: 10.4085/1062-6050-43.2.215 PubMed DOI PMC

Billat VL, Hill DW, Pinoteau J, Petit B, Koralsztein JP. Effect of protocol on determination of velocity at VO2max and on its time to exhaustion. Arch Physiol Biochem. 1996;104(3):313–21. doi: 10.1076/apab.104.3.313.12908 PubMed DOI

Howley ET, Bassett DR, Welch HG. Criteria for maximal oxygen uptake: Review and commentary. Med Sci Sports Exerc. 1995;27(9):1292–301. PubMed

Midgley AW, McNaughton LR, Polman R, Marchant D. Criteria for determination of maximal oxygen uptake: A brief critique and recommendations for future research. Sport Med. 2007;37(12):1019–28. doi: 10.2165/00007256-200737120-00002 PubMed DOI

Millet GP, Candau R, Fattori P, Bignet F, Varray A. VO2 responses to different intermittent runs at velocity associated with VO2max. Can J Appl Physiol. 2003;28(3):410–23. doi: 10.1139/h03-030 PubMed DOI

Nicolson GL, de Mattos GF, Settineri R, Costa C, Ellithorpe R, Rosenblatt S, et al.. Clinical effects of hydrogen administration: From animal and human diseases to exercise medicine. Int J Clin Med. 2016;7:32–76.

Hopkins WG. Spreadsheets for analysis of validity and reliability. Sportscience. 2015;19:36–42.

Hopkins WG, Marshall SW, Batterham AM, Hanin J. Progressive statistics for studies in sports medicine and exercise science. Med Sci Sports Exerc. 2009;41(1):3–13. doi: 10.1249/MSS.0b013e31818cb278 PubMed DOI

Malcata RM, Hopkins WG. Variability of competitive performance of elite athletes: A systematic review. Sport Med. 2014;44(12):1763–74. doi: 10.1007/s40279-014-0239-x PubMed DOI

Hinckson EA, Hopkins WG. Reliability of time to exhaustion analyzed with critical-power and log-log modeling. Med Sci Sports Exerc. 2005;37(4):696–701. doi: 10.1249/01.mss.0000159023.06934.53 PubMed DOI

Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007;39(2):175–91. doi: 10.3758/bf03193146 PubMed DOI

Billat V, Beillot J, Jan J, Rochcongar P, Carre F. Gender effect on the relationship of time limit at 100% VO2max with other bioenergetic characteristics. Med Sci Sports Exerc. 1996;28(8):1049–55. doi: 10.1097/00005768-199608000-00016 PubMed DOI

Laursen P, Buchheit M. Science and application of high-intensity interval training: Solutions to the programming puzzle. Champaign, IL, USA: Human Kinetics; 2019. PubMed

Billat V, Renoux JC, Pinoteau J, Petit B, Koralsztein JP. Times to exhaustion at 100% of velocity at VO2max and modelling of the time-limit/velocity relationship in elite long-distance runners. Eur J Appl Physiol Occup Physiol. 1994;69(3):271–3. doi: 10.1007/BF01094801 PubMed DOI

Renoux JC, Petit B, Billat V, Koralsztein JP. Oxygen deficit is related to the exercise time to exhaustion at maximal aerobic speed in middle distance runners. Arch Physiol Biochem. 1999;107(4):280–5. doi: 10.1076/13813455199908107041qft280 PubMed DOI

Robergs RA, Ghiasvand F, Parker D. Biochemistry of exercise-induced metabolic acidosis. Am J Physiol. 2004;287(3):R502–R516. doi: 10.1152/ajpregu.00114.2004 PubMed DOI

Brooks GA. The science and translation of lactate shuttle theory. Cell Metab. 2018;27(4):757–85. doi: 10.1016/j.cmet.2018.03.008 PubMed DOI

Radak Z, Marton O, Nagy E, Koltai E, Goto S. The complex role of physical exercise and reactive oxygen species on brain. J Sport Heal Sci. 2013;2(2):87–93.

He F, Li J, Liu Z, Chuang CC, Yang W, Zuo L. Redox mechanism of reactive oxygen species in exercise. Front Physiol. 2016;7(486):1–10. doi: 10.3389/fphys.2016.00486 PubMed DOI PMC

Çakır-Atabek H, Özdemir F, Çolak R. Oxidative stress and antioxidant responses to progressive resistance exercise intensity in trained and untrained males. Biol Sport. 2015;32(4):321–8. doi: 10.5604/20831862.1176302 PubMed DOI PMC

Nogueira JE, Amorim MR, Pinto AP, da Rocha AL, da Silva ASR, Branco LGS. Molecular hydrogen downregulates acute exhaustive exercise-induced skeletal muscle damage. Can J Physiol Pharmacol. 2021;99(8):812–20. doi: 10.1139/cjpp-2020-0297 PubMed DOI

Laursen PB, Francis GT, Abbiss CR, Newton MJ, Nosaka K. Reliability of time-to-exhaustion versus time-trial running tests in runners. Med Sci Sports Exerc. 2007;39(8):1374–9. doi: 10.1249/mss.0b013e31806010f5 PubMed DOI

Hopkins WG, Schabort EJ, Hawley JA. Reliability of power in physical performance tests. Sport Med. 2001;31(3):211–34. doi: 10.2165/00007256-200131030-00005 PubMed DOI

Hydrogen-rich water supplementation promotes muscle recovery after two strenuous training sessions performed on the same day in elite fin swimmers: randomized, double-blind, placebo-controlled, crossover trial