Vascular wilt caused by the ascomycete fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a major constraint of banana production around the world. The virulent race, namely Tropical Race 4, can infect all Cavendish-type banana plants and is now widespread across the globe, causing devastating losses to global banana production. In this study, we characterized Foc Subtropical Race 4 (STR4) resistance in a wild banana relative which, through estimated genome size and ancestry analysis, was confirmed to be Musa acuminata ssp. malaccensis. Using a self-derived F2 population segregating for STR4 resistance, quantitative trait loci sequencing (QTL-seq) was performed on bulks consisting of resistant and susceptible individuals. Changes in SNP index between the bulks revealed a major QTL located on the distal end of the long arm of chromosome 3. Multiple resistance genes are present in this region. Identification of chromosome regions conferring resistance to Foc can facilitate marker assisted selection in breeding programs and paves the way towards identifying genes underpinning resistance.

Australian Genome Research Facility Victorian Comprehensive Cancer Centre Melbourne VIC 3000 Australia

Centre of the Region Haná for Biotechnological and Agricultural Research Institute of Experimental Botany of the Czech Academy of Sciences 77200 Olomouc Czech Republic

CIRAD UMR AGAP Institut F 34398 Montpellier France

Department of Agriculture and Fisheries Maroochy Research Facility Nambour QLD 4560 Australia

Department of Microbiology and Immunology Peter Doherty Institute for Infection and Immunity University of Melbourne Melbourne VIC 3004 Australia

Genome Innovation Hub University of Queensland Brisbane QLD 4072 Australia

Inari Agriculture West Lafayette IN 47906 USA

International Institute of Tropical Agriculture Kampala P O Box 7878 Uganda

Laboratory of Tropical Crop Improvement Division of Crop Biotechnics Katholieke Universiteit Leuven 3001 Leuven Belgium

School of Agriculture and Food Science The University of Queensland Brisbane QLD 4067 Australia

School of Biological Sciences The University of Western Australia Perth WA 6907 Australia

School of Life Science Jiaying University Meizhou 514015 China

UMR AGAP Institut Univ Montpellier CIRAD INRAE Institut Agro F 34398 Montpellier France

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Genetic Mapping, Candidate Gene Identification and Marker Validation for Host Plant Resistance to the Race 4 of Fusarium oxysporum f. sp. cubense Using Musa acuminata ssp. malaccensis