The Effects of Quercetin on Apoptosis and Antioxidant Activity in a Renal Ischemia/Reperfusion Injury Animal Model
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
36972618
DOI
10.1055/a-1999-7600
Knihovny.cz E-resources
- MeSH
- Acute Kidney Injury * drug therapy metabolism MeSH
- Antioxidants metabolism MeSH
- Apoptosis MeSH
- Cytokines metabolism MeSH
- Ischemia complications metabolism MeSH
- Rats MeSH
- Kidney MeSH
- NF-kappa B metabolism pharmacology therapeutic use MeSH
- Oxidative Stress MeSH
- Rats, Wistar MeSH
- Quercetin pharmacology therapeutic use MeSH
- Reperfusion Injury * drug therapy metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- Cytokines MeSH
- NF-kappa B MeSH
- Quercetin MeSH
Renal ischemia-reperfusion injury (IRI) is considered as one of the most prevalent causes of acute kidney injury (AKI), which can happen in various clinical situations including hypovolemic shock, injury, thrombo-embolism, and after a kidney transplant. This paper aims to evaluate the reno-protective effects of Quercetin in induced ischemia/reperfusion injury by regulating apoptosis-related proteins, inflammatory cytokines, MMP-2, MMP-9, and nuclear factor kappa-light-chain-enhancer inactivated B cells (NF-kB) in rats. The male Wistar rats (n=32) were randomly divided into Sham, untreated IR, and Quercetin-treated IR (gavage and intraperitoneal). Quercetin was given orally and intraperitoneally one hour before inducing ischemia-reperfusion injury . After reperfusion, blood samples and kidneys were collected to assess renal function and inflammatory cytokines, apoptotic signaling proteins, and antioxidants. Urea, creatinine, and MDA levels improved in Quercetin-treated groups with different administration methods. In addition, the activities of other antioxidant in Quercetin-treated rats were higher than those in the IR group. Further, Quercetin inhibited NF-kB signaling, apoptosis-associated factors and produced matrix metalloproteinase protein in the kidneys of rats. Based on the findings, the antioxidant, anti-inflammatory, and anti-apoptotic effects of the Quercetin diminished renal ischemia-reperfusion injury in the rats significantly. It is suggested that a single dosage of Quercetin have a reno-protective impact in the case of renal I/R injury.
Department of Biology Faculty of Science Ferdowsi University of Mashhad Mashhad Iran
Department of Microbiology School of Biology College of Science University of Tehran Tehran Iran
Department of Urology Sina Hospital Tabriz University of Medical Sciences Tabriz Iran
Faculty of Pharmacy in Hradec Kralove Charles University Prague Czech Republic
Kidney Research Center Tabriz University of Medical Sciences Tabriz Iran
Network of Immunity in Infection Malignancy and Autoimmunity Tehran Iran
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