A new subspecies of Leptideasinapis from Northern Iran, discovered by means of DNA barcoding, is described as Leptideasinapistabarestanassp. nov. The new subspecies is allopatric with respect to other populations of L.sinapis and is genetically distinct, appearing as a well-supported sister clade to all other populations in COI-based phylogenetic reconstructions. Details on karyotype, genitalia, ecology and behaviour for the new subspecies are given and a biogeographical speciation scenario is proposed.

Department of Biodiversity and Conservation Biology Institute of Entomology Biology Centre of the Czech Academy of Sciences České Budějovice Czech Republic Institute of Entomology Biology Centre of the Czech Academy of Sciences České Budějovice Czech Republic

Department of Biology University of Padova Padova Italy University of Padova Padova Italy

Department of Karyosystematics Zoological Institute of Russian Academy of Science Universitetskaya nab 1 199034 St Petersburg Russia Zoological Institute of Russian Academy of Science St Petersburg Russia

Ecology and Genetics Research Unit University of Oulu Oulu Finland University of Oulu Oulu Finland

Institut de Biologia Evolutiva Barcelona Spain Universitat Pompeu Fabra Barcelona Spain

National Natural History Museum and Genetic Resources Tehran Iran National Natural History Museum and Genetic Resources Tehran Iran

Research Institute of the University of Bucharest University of Bucharest Bucharest Romania University of Bucharest Bucharest Romania

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