Lyme disease, caused by some strains of bacterial spirochetes Borrelia burgdorferi sensu lato (Bbsl), affects humans but also domestic animals including horses. The primary pathogens in horses in Europe are B. afzelii, B. garinii and B. burgdorferi sensu stricto. To our knowledge, there are no data available on the seropositivity of B. burgdorferi s.l. in horses from the Czech Republic. In this country, horses are mainly used for sport, breeding, and recreational riding in areas where vectors of B. burgdorferi s.l. are present, which is why they are frequently at risk of infection. The aim of the study was to detect anti-borrelia IgM and IgG antibodies in clinically healthy and sick horses from the Czech Republic and to evaluate the risk factors of infection. In total, sera of 262 horses (247 clinically healthy horses and 15 horses hospitalized due to symptoms of encephalitis/meningoencephalitis) were examined by an indirect sandwich enzyme-linked immunosorbent assay. Positivity of B. burgdorferi was 27% (66/247) in clinically healthy horses (21% IgM, 7% IgG and 3% IgM + IgG antibodies) and 20% (3/15) in horses with clinical signs (20% IgM, 7% IgG and 7% IgM + IgG). In the clinically healthy horses, positivity statistically differed (p ≤ 0.05) only in Pony and Warmblood breeds, being the most affected at 32% and 30%, respectively, while other characteristics (sex, age, usage and localities) had no effect on positivity. This is the first survey of antibodies to B. burgdorferi s.l. in Czech horses showing that horses are exposed to ticks infected with B. burgdorferi s.l. This should be taken into account when making differential diagnoses in patients with non-specific symptoms to start with adequate therapy.

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary Sciences Brno Palackého tř 1946 1 612 42 Brno Czech Republic

Department of Biology Faculty of Education Masaryk University Poříčí 7 9 625 00 Brno Czech Republic

Department of Experimental Biology Faculty of Science Masaryk University Kamenice 5 625 00 Brno Czech Republic

Department of Mathematics and Statistics Faculty of Science Masaryk University Kotlářská 267 2 611 37 Brno Czech Republic

State Veterinary Institute Prague Sídlištní 136 24 165 03 Prague Czech Republic

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Girschick H.J., Morbach H., Tappe D. Treatment of Lyme borreliosis. Arthritis Res. Ther. 2009;11:258. doi: 10.1186/ar2853. PubMed DOI PMC

Manzoor K., Aftab W., Choksi S., Khan I.A. Lyme carditis: Sequential electrocardiographic changes in response to antibiotic therapy. Int. J. Cardiol. 2009;137:167–171. doi: 10.1016/j.ijcard.2008.05.028. PubMed DOI

Jaenson T.G., Lindgren E. The range of Ixodes ricinus and the risk of contracting Lyme borreliosis will increase northwards when the vegetation period becomes longer. Ticks Tick Borne Dis. 2011;2:44–49. doi: 10.1016/j.ttbdis.2010.10.006. PubMed DOI

Capinera J.L. Encyclopedia of Entomology. Springer Science & Business Media; Heidelberg, Germany: 2008. 4411p

Stanek G., Wormser G.P., Gray J., Strle F. Lyme borreliosis. Lancet. 2012;379:461–473. doi: 10.1016/S0140-6736(11)60103-7. PubMed DOI

Sonenshine D.E., Roe R.M. Biology of Ticks Volume 2. OUP USA; Oxford, UK: 2003. 504p

Lindgren E., Jaenson T.G.T. Lyme Borreliosis in Europe: Influences of Climate Change, Epidemiology, Ecology and Adaptation Measures. World Health Organization; Geneva, Switzerland: 2006. p. 22.

Ginsberg H.S., Albert M., Acevedo L., Dyer M.C., Arsnoe I.M., Tsao J.I., Mather T.N., LeBrun R.A. Environmental factors affecting survival of immature Ixodes scapularis and implications for geographical distribution of Lyme Disease: The climate/behavior hypothesis. PLoS ONE. 2017;12:e0168723. doi: 10.1371/journal.pone.0168723. PubMed DOI PMC

Browning A., Carter S.D., Barnes A., May C., Bennett D. Lameness associated with Borrelia burgdorferi infection in the horse. Vet. Rec. 1993;132:610–611. doi: 10.1136/vr.132.24.610. PubMed DOI

James F.M., Engiles J.B., Beech J. Meningitis, cranial neuritis, and radiculoneuritis associated with Borrelia burgdorferi infection in a horse. JAVMA. 2010;237:1180–1185. doi: 10.2460/javma.237.10.1180. PubMed DOI

Štefančíková A., Derdáková M., Škardová I., Szestáková E., Cisláková L., Kováčová D., Stanko M., Petko B. Prevalence of antibodies to Borrelia burgdorferi in horses of east Slovakia. Vet. Med. 2000;45:227–231.

Štefančíková A., Adaszek Ł., Peťko B., Winiarczyk S., Dudiňák V. Serological evidence of Borrelia burgdorferi sensu lato in horses and cattle from Poland and diagnostic problems of Lyme borreliosis. Ann. Agric. Environ. Med. 2008;15:37–43. PubMed

Daniel M., Materna J., Honig V., Metelka L., Danielová V., Harcarik J., Kliegerová S., Grubhoffer L. Vertical distribution of the tick Ixodes ricinus and tick-borne pathogens in the northern Moravian mountains correlated with climate warming (Jeseníky Mts., Czech Republic) Cent. Eur. J. Public Health. 2009;17:139–145. doi: 10.21101/cejph.a3550. PubMed DOI

Hönig V., Svec P., Halas P., Vavruskova Z., Tykalova H., Kilian P., Vetiskova V., Dornakova V., Sterbova J., Simonova Z., et al. Ticks and tick-borne pathogens in South Bohemia (Czech Republic)—Spatial variability in Ixodes ricinus abundance, Borrelia burgdorferi and tick-borne encephalitis virus prevalence. Ticks Tick Borne Dis. 2015;6:559–567. doi: 10.1016/j.ttbdis.2015.04.010. PubMed DOI

Bonczek O., Žákovská A., Vargová L., Šerý O. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. Ann. Agric. Environ. Med. 2015;22:637–641. doi: 10.5604/12321966.1185766. PubMed DOI

Kučerová H.L., Žákovská A., Marková J., Bártová E. Detection of antibodies to Borrelia burgdorferi s.l. in wild small mammals and senzitivity of PCR and cultivation. Vet. Microbiol. 2019;230:241–243. doi: 10.1016/j.vetmic.2019.02.004. PubMed DOI

StatSoft, Inc. STATISTICA (Data Analysis Software System), Version 12. 2013. [(accessed on 20 August 2021)]. Available online: http://www.statsoft.com/Products/STATISTICA-Features.

Hansen M.G., Christoffersen M., Thuesen L.R., Petersen M.R., Bojesen A.M. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in Danish horses. Acta Vet. Scand. 2010;52:3. doi: 10.1186/1751-0147-52-3. PubMed DOI PMC

Ebani V.V., Bertelloni F., Pinzauti P., Cerri D. Seroprevalence of Leptospira spp. and Borrelia burgdorferi sensu lato in Italian horses. Ann. Agric. Environ. Med. 2012;19:237–340. PubMed

Socoloski S.N.G., de Castro B.G., Cordeiro M.D., da Fonseca A.H., Cepeda M.B., Nicolino R.R., Lopes L.B. Epidemiological investigation of Borrelia burgdorferi in horses in the municipality of Sinop-MT, Brazil. Trop. Anim. Health Prod. 2018;50:831–836. doi: 10.1007/s11250-017-1504-4. PubMed DOI

Burbelo P.D., Bren K.E., Ching K.H., Coleman A., Yang X., Kariu T., Iadarola M.J., Pal U. Antibody profiling of Borrelia burgdorferi infection in horses. Clin. Vaccine Immunol. 2011;18:1562–1567. doi: 10.1128/CVI.05123-11. PubMed DOI PMC

Kiss T., Cadar D., Krupaci A.F., Bordeanu A., Brudasca G.F., Mihalca A.D., Mircean V., Glica L., Dumitrache M.O., Spinu M. Serological reactivity to Borrelia burgdorferi sensu lato in dogs and horses from distinct areas in Romania. Vector Borne Zoonotic Dis. 2011;11:1259–1262. doi: 10.1089/vbz.2010.0254. PubMed DOI

Lee S.H., Yun S.H., Choi E., Park Y.S., Lee S.E., Cho G.J., Kwon O.D., Kwak D. Serological detection of Borrelia burgdorferi among horses in Korea. Korean J. Parasitol. 2016;54:97–101. doi: 10.3347/kjp.2016.54.1.97. PubMed DOI PMC

Divers T.J., Gardner R.B., Madigan J.E., Witonsky S.G., Bertone J.J., Swinebroad E.L., Schutzer S.E., Johnson A.L. Borrelia burgdorferi infection and Lyme disease in North American horses: A consensus statement. J. Vet. Intern. Med. 2018;32:617–632. doi: 10.1111/jvim.15042. PubMed DOI PMC

Laamari A., Azzag N., Tennah S., Derdour S.Y., China B., Bouabdallah R., Ghalmi F. Seroprevalence of antibodies against Anaplasma phagocytophilum and Borrelia burgdorferi in horses (Equus caballus) from Northern Algeria. J. Vet. Res. 2020;64:413–419. doi: 10.2478/jvetres-2020-0045. PubMed DOI PMC

Egenvall A., Franzén P., Gunnarsson A., Engvall E.O., Vågsholm I., Wikström U.B., Artursson K. Cross-sectional study of the seroprevalence to Borrelia burgdorferi sensu lato and granulocytic Ehrlichia spp. and demographic, clinical and tick-exposure factors in Swedish horses. Prev. Vet. Med. 2001;49:191–208. doi: 10.1016/S0167-5877(01)00187-8. PubMed DOI

Magnarelli L.A., Anderson J.F., Shaw E., Post J.E., Palka F.C. Borreliosis in equids in northeastern United States. Am. J. Vet. Res. 1988;49:359–362. PubMed

Appel M.J.G. Lyme disease in dogs and cats. Compendium. 1990;12:617–624.

Imai D.M., Barr B.C., Bertone J.J., Feng S., Hodzic E., Johnston J.M., Olsen K.J., Barthold S.W. Lyme Neuroborreliosis in 2 horses. Vet. Pathol. 2011;48:1151. doi: 10.1177/0300985811398246. PubMed DOI

Priest H.L., Irby N.L., Schlafer D.H., Divers T.J., Wagner B., Glaser A.L., Chang Y.F., Smith M.C. Diagnosis of Borrelia-associated uveitis in two horses. Vet. Ophtalmol. 2012;15:98–405. doi: 10.1111/j.1463-5224.2012.01000.x. PubMed DOI

Steere A.C., Schoen R.T., Taylor E. The clinical evolution of Lyme arthritis. Ann. Inter. Med. 1987;107:725–731. doi: 10.7326/0003-4819-107-5-725. PubMed DOI

Madigan J.E. Lyme disease (Lyme borreliosis) in horses. Vet. Clin. N. Am. Equine Pract. 1993;9:429–434. doi: 10.1016/S0749-0739(17)30409-1. PubMed DOI

Shapiro E.D. Lyme disease. Adv. Exp. Med. Biol. 2008;609:185–195. PubMed

Daniel M., Rudenko N., Golovchenko M., Danielová V., Fialová A., Kříž B., Malý M. The occurrence of Ixodes ricinus ticks and important tick-borne pathogens in areas with high tick-borne encephalitis prevalence in different altitudinal levels of the Czech Republic. Part II. Ixodes ricinus ticks and genospecies of Borrelia burgdorferi sensu lato complex. Epidemiol. Mikrobiol. Immunol. 2016;65:182–192. PubMed

Postic D., Garnier M., Baranton G. Multilocus sequence analysis of atypical Borrelia burgdorferi sensu lato isolates—description of Borrelia californiensis sp. nov, and genomospecies 1 and 2. Int. J. Med. Microbiol. 2007;297:263–271. doi: 10.1016/j.ijmm.2007.01.006. PubMed DOI

Rudenko N., Golovchenko M., Mokra A., Piskunova N., Růžek D., Mallatova N., Grubhoffer L. Detection of Borrelia bissettii in cardiac valve tissue of a patient with endocarditis and aortic valve stenosis in the Czech Republic. J. Clin. Microb. 2008;46:3540–3543. doi: 10.1128/JCM.01032-08. PubMed DOI PMC