Binder H33 is a small protein binder engineered by ribosome display to bind human interleukin 10. Crystals of binder H33 display severe diffraction anisotropy. A set of data files with correction for diffraction anisotropy based on different local signal-to-noise ratios was prepared. Paired refinement was used to find the optimal anisotropic high-resolution diffraction limit of the data: 3.13-2.47 Å. The structure of binder H33 belongs to the 2% of crystal structures with the highest solvent content in the Protein Data Bank.

Czech Technical University Prague Brehova 7 Prague 115 19 Czech Republic

Institute of Biotechnology of the Czech Academy of Sciences Biocev Průmyslová 595 Vestec 25250 Czech Republic

Zobrazit více v PubMed

Agirre, J., Atanasova, M., Bagdonas, H., Ballard, C. B., Baslé, A., Beilsten-Edmands, J., Borges, R. J., Brown, D. G., Burgos-Mármol, J. J., Berrisford, J. M., Bond, P. S., Caballero, I., Catapano, L., Chojnowski, G., Cook, A. G., Cowtan, K. D., Croll, T. I., Debreczeni, J. É., Devenish, N. E., Dodson, E. J., Drevon, T. R., Emsley, P., Evans, G., Evans, P. R., Fando, M., Foadi, J., Fuentes-Montero, L., Garman, E. F., Gerstel, M., Gildea, R. J., Hatti, K., Hekkelman, M. L., Heuser, P., Hoh, S. W., Hough, M. A., Jenkins, H. T., Jiménez, E., Joosten, R. P., Keegan, R. M., Keep, N., Krissinel, E. B., Kolenko, P., Kovalevskiy, O., Lamzin, V. S., Lawson, D. M., Lebedev, A. A., Leslie, A. G. W., Lohkamp, B., Long, F., Malý, M., McCoy, A. J., McNicholas, S. J., Medina, A., Millán, C., Murray, J. W., Murshudov, G. N., Nicholls, R. A., Noble, M. E. M., Oeffner, R., Pannu, N. S., Parkhurst, J. M., Pearce, N., Pereira, J., Perrakis, A., Powell, H. R., Read, R. J., Rigden, D. J., Rochira, W., Sammito, M., Sánchez Rodríguez, F., Sheldrick, G. M., Shelley, K. L., Simkovic, F., Simpkin, A. J., Skubak, P., Sobolev, E., Steiner, R. A., Stevenson, K., Tews, I., Thomas, J. M. H., Thorn, A., Valls, J. T., Uski, V., Usón, I., Vagin, A., Velankar, S., Vollmar, M., Walden, H., Waterman, D., Wilson, K. S., Winn, M. D., Winter, G., Wojdyr, M. & Yamashita, K. (2023). Acta Cryst. D79, 449–461.

Berman, H., Henrick, K. & Nakamura, H. (2003). Nat. Struct. Mol. Biol. 10, 980. PubMed

Brünger, A. T. (1992). Nature, 355, 472–475. PubMed

Chen, V. B., Arendall, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S. & Richardson, D. C. (2010). Acta Cryst. D66, 12–21. PubMed PMC

Cianci, M., Bourenkov, G., Pompidor, G., Karpics, I., Kallio, J., Bento, I., Roessle, M., Cipriani, F., Fiedler, S. & Schneider, T. R. (2017). J. Synchrotron Rad. 24, 323–332. PubMed PMC

El Kasmi, K. C., Smith, A. M., Williams, L., Neale, G., Panopolous, A., Watowich, S. S., Häcker, H., Foxwell, B. M. & Murray, P. J. (2007). J. Immunol. 179, 7215–7219. PubMed

Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. (2010). Acta Cryst. D66, 486–501. PubMed PMC

Evans, P. R. & Murshudov, G. N. (2013). Acta Cryst. D69, 1204–1214. PubMed PMC

Hořejší, Z., Stach, L., Flower, T. G., Joshi, D., Flynn, H., Skehel, J. M., O’Reilly, N. J., Ogrodowicz, R. W., Smerdon, S. J. & Boulton, S. J. (2014). Cell. Rep. 7, 19–26. PubMed PMC

Joosten, R. P., Long, F., Murshudov, G. N. & Perrakis, A. (2014). IUCrJ, 1, 213–220. PubMed PMC

Kabsch, W. (2010). Acta Cryst. D66, 125–132. PubMed PMC

Kantardjieff, K. A. & Rupp, B. (2003). Protein Sci. 12, 1865–1871. PubMed PMC

Karplus, P. A. & Diederichs, K. (2012). Science, 336, 1030–1033. PubMed PMC

Karplus, P. A. & Diederichs, K. (2015). Curr. Opin. Struct. Biol. 34, 60–68. PubMed PMC

Malý, M., Diederichs, K., Dohnálek, J. & Kolenko, P. (2020). IUCrJ, 7, 681–692. PubMed PMC

Malý, M., Diederichs, K., Dohnálek, J. & Kolenko, P. (2021). Acta Cryst. F77, 226–229. PubMed PMC

McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C. & Read, R. J. (2007). J. Appl. Cryst. 40, 658–674. PubMed PMC

McNicholas, S., Potterton, E., Wilson, K. S. & Noble, M. E. M. (2011). Acta Cryst. D67, 386–394. PubMed PMC

Murshudov, G. N., Skubák, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Long, F. & Vagin, A. A. (2011). Acta Cryst. D67, 355–367. PubMed PMC

Nicholls, R. A., Long, F. & Murshudov, G. N. (2012). Acta Cryst. D68, 404–417. PubMed PMC

Pham, P. N., Huličiak, M., Biedermannová, L., Černý, J., Charnavets, T., Fuertes, G., Herynek, Š., Kolářová, L., Kolenko, P., Pavlíček, J., Zahradník, J., Mikulecký, P. & Schneider, B. (2021). Viruses, 13, 190. PubMed PMC

Robert, X., Kassis-Sahyoun, J., Ceres, N., Martin, J., Sawaya, M. R., Read, R. J., Gouet, P., Falson, P. & Chaptal, V. (2017). Sci. Rep. 7, e17013. PubMed PMC

Rupp, B. (2018). Structure, 26, 919–923. PubMed

Strong, M., Sawaya, M. R., Wang, S., Phillips, M., Cascio, D. & Eisenberg, D. (2006). Proc. Natl Acad. Sci. USA, 103, 8060–8065. PubMed PMC

Tickle, I. J., Flensburg, C., Keller, P., Paciorek, W., Sharff, A., Vonrhein, C. & Bricogne, G. (2018). STARANISO. Global Phasing Ltd, Cambridge, UK. https://staraniso.globalphasing.org/cgi-bin/staraniso.cgi.

Urzhumtsev, A., Afonine, P. V., Lunin, V. Y., Terwilliger, T. C. & Adams, P. D. (2014). Acta Cryst. D70, 2593–2606. PubMed PMC

Winn, M. D., Ballard, C. C., Cowtan, K. D., Dodson, E. J., Emsley, P., Evans, P. R., Keegan, R. M., Krissinel, E. B., Leslie, A. G. W., McCoy, A., McNicholas, S. J., Murshudov, G. N., Pannu, N. S., Potterton, E. A., Powell, H. R., Read, R. J., Vagin, A. & Wilson, K. S. (2011). Acta Cryst. D67, 235–242. PubMed PMC