Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Wellcome Trust - United Kingdom
PubMed
37669165
DOI
10.1016/j.celrep.2023.113083
PII: S2211-1247(23)01094-X
Knihovny.cz E-resources
- Keywords
- CP: Cell biology, Trypanosoma, fucose, inner mitochondrial membrane, kinetoplast, mitochondrion, protein targeting,
- MeSH
- Biology MeSH
- Mitochondrial Proteins metabolism MeSH
- Mitochondria metabolism MeSH
- Parasites * metabolism MeSH
- Protozoan Proteins metabolism MeSH
- Trypanosoma brucei brucei * metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Mitochondrial Proteins MeSH
- Protozoan Proteins MeSH
We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procyclic stage containing 1,239 proteins. For 337 of these, a mitochondrial localization had not been described before. We use the TrypTag dataset as a foundation and take advantage of the properties of the fluorescent protein tag that causes aberrant but fortuitous accumulation of tagged matrix and inner membrane proteins near the kinetoplast (mitochondrial DNA). Combined with transmembrane domain predictions, this characteristic allowed categorization of 1,053 proteins into mitochondrial sub-compartments, the detection of unique matrix-localized fucose and methionine synthesis, and the identification of new kinetoplast proteins, which showed kinetoplast-linked pyrimidine synthesis. Moreover, disruption of targeting signals by tagging allowed mapping of the mode of protein targeting to these sub-compartments, identifying a set of C-tail anchored outer mitochondrial membrane proteins and mitochondrial carriers likely employing multiple target peptides. This dataset represents a comprehensive, updated mapping of the mitochondrion.
Division of Biomedical Sciences Warwick Medical School University of Warwick Coventry UK
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
Oxford Brookes University Oxford UK
Sir William Dunn School of Pathology University of Oxford Oxford UK
References provided by Crossref.org
Distribution and Functional Analysis of Isocitrate Dehydrogenases across Kinetoplastids
Genomics of Preaxostyla Flagellates Illuminates the Path Towards the Loss of Mitochondria
