Integrative CUT&Tag-RNA-Seq analysis of histone variant macroH2A1-dependent orchestration of human induced pluripotent stem cell reprogramming
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
37846557
DOI
10.2217/epi-2023-0267
Knihovny.cz E-resources
- Keywords
- CUT&Tag, iPSCs, induced pluripotent stem cells, macroH2A1, reprogramming, somatic cells,
- MeSH
- Endothelial Cells metabolism MeSH
- Histones * metabolism MeSH
- Induced Pluripotent Stem Cells * metabolism MeSH
- Humans MeSH
- Cellular Reprogramming genetics MeSH
- RNA-Seq MeSH
- Transcription Factors genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Histones * MeSH
- Transcription Factors MeSH
Aim: Human induced pluripotent stem cells (iPSCs) are inefficiently derived from somatic cells by overexpression of defined transcription factors. Overexpression of H2A histone variant macroH2A1.1, but not macroH2A1.2, leads to increased iPSC reprogramming by unclear mechanisms. Materials & methods: Cleavage under targets and tagmentation (CUT&Tag) allows robust epigenomic profiling of a low cell number. We performed an integrative CUT&Tag-RNA-Seq analysis of macroH2A1-dependent orchestration of iPSCs reprogramming using human endothelial cells. Results: We demonstrate wider genome occupancy, predicted transcription factors binding, and gene expression regulated by macroH2A1.1 during reprogramming, compared to macroH2A1.2. MacroH2A1.1, previously associated with neurodegenerative pathologies, specifically activated ectoderm/neural processes. Conclusion: CUT&Tag and RNA-Seq data integration is a powerful tool to investigate the epigenetic mechanisms occurring during cell reprogramming.
Bioinformatics Unit Fondazione IRCCS Casa Sollievo della Sofferenza 71013 San Giovanni Rotondo Italy
Department of Biomedical and Biotechnological Sciences University of Catania 95123 Catania Italy
Faculty of Health Liverpool John Moores University L2 2ER Liverpool UK
Institute of Experimental Biology Faculty of Science Masaryk University 62500 Brno Czech Republic
International Clinical Research Center St Anne's University Hospital 65691 Brno Czech Republic
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