Associations of Serum Total Homocysteine Levels with Various Demographic, Clinical and Genetic Characteristics in Healthy Greek Adults
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
37930095
DOI
10.14712/18059694.2023.17
PII: am_2023066020061
Knihovny.cz E-resources
- Keywords
- MTHFR A1298C, MTHFR C677T, cobalamin, folate, homocysteine,
- MeSH
- Demography MeSH
- Adult MeSH
- Genotype MeSH
- Homocysteine genetics MeSH
- Folic Acid * MeSH
- Humans MeSH
- Methylenetetrahydrofolate Reductase (NADPH2) genetics MeSH
- Polymorphism, Genetic * MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Greece MeSH
- Names of Substances
- Homocysteine MeSH
- Folic Acid * MeSH
- Methylenetetrahydrofolate Reductase (NADPH2) MeSH
AIM: The aim of this study was to investigate the association of serum total Hcy (tHcy) levels with various demographic, clinical and genetic characteristics in healthy Greek adults. METHODS: Anthropometric characteristics (height, weight), systolic and diastolic blood pressure, complete blood count and biochemical assessments, were recorded and measured among 383 Greek adults (199 men). Serum folate, Cobalamin (Cbl) and tHcy levels were determined using immunoassays methods. The MTHFR C677T and A1298C gene polymorphisms were genotyped using polymerase chain reaction and reverse hybridization. RESULTS: MTHFR C677T gene polymorphism, serum folate and Cbl levels were correlated with serum tHcy levels independently. The individuals with 677TT genotype had significantly higher serum tHcy levels than individuals with 677 CC or CT genotypes. Regarding the MTHFR C677T gene polymorphism, the existence of the T allele was associated with statistically significantly lower serum folate and higher serum tHcy levels than C allele. Regarding the MTHFR A1298C gene polymorphism, the existence of the C allele was associated with statistically significant lower serum tHcy levels than A allele. Furthermore, there was no significant correlation between the serum tHcy levels and demographic (except age) or clinical characteristics (sex, BMI, smoking status, SBP, DBP, HGB, HCT, TC, TG, HDL-C, LDL-C, TC/HDL-C). CONCLUSIONS: Serum tHcy levels are influenced by the existence of MTHFR C677T gene polymorphism (mainly 677TT genotype), serum folate and Cbl levels. Individuals with hyperhomocysteinemia should be further investigated for the existence of MTHFR C677T gene polymorphism, with the aim to determine the suitable treatment.
Department of Internal Medicine Naval Hospital of Crete Chania Greece
Department of Internal Medicine University Hospital of Heraklion Heraklion Greece
Private Medical Office of Internal Medicine Chania Greece
School of Production Engineering and Management Technical University of Crete Chania Greece
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