TGF-β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
37944712
DOI
10.1016/j.matbio.2023.11.001
PII: S0945-053X(23)00113-0
Knihovny.cz E-zdroje
- Klíčová slova
- Cancer, Epithelial-to-mesenchymal transition (EMT), Extracellular matrix (ECM), Prostate, Transforming growth factor (TGF)-β, Tumoroids, Tumour,
- MeSH
- epitelo-mezenchymální tranzice MeSH
- extracelulární matrix metabolismus MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory prostaty * patologie MeSH
- prostata metabolismus MeSH
- transformující růstový faktor beta * metabolismus MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- transformující růstový faktor beta * MeSH
Extracellular matrix (ECM) tumorigenic alterations resulting in high matrix deposition and stiffening are hallmarks of adenocarcinomas and are collectively defined as desmoplasia. Here, we thoroughly analysed primary prostate cancer tissues obtained from numerous patients undergoing radical prostatectomy to highlight reproducible structural changes in the ECM leading to the loss of the glandular architecture. Starting from patient cells, we established prostate cancer tumoroids (PCTs) and demonstrated they require TGF-β signalling pathway activity to preserve phenotypical and structural similarities with the tissue of origin. By modulating TGF-β signalling pathway in PCTs, we unveiled its role in ECM accumulation and remodelling in prostate cancer. We also found that TGF-β-induced ECM remodelling is responsible for the initiation of prostate cell epithelial-to-mesenchymal transition (EMT) and the acquisition of a migratory, invasive phenotype. Our findings highlight the cooperative role of TGF-β signalling and ECM desmoplasia in prompting prostate cell EMT and promoting tumour progression and dissemination.
Department of Computer Engineering Modelling Electronics and Systems Engineering 87036 Italy
Department of Engineering for Innovation University of Salento Italy
Department of Urology St Anne's University Hospital Brno 60200 Czech Republic
Information Technology Center 87036 Italy
International Clinical Research Center St Anne's University Hospital Brno 60200 Czech Republic
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