Study of the Methylation of Bovine GSTP1 Gene under the Influence of Pesticide Mospilan 20SP Alone and in Combination with Pesticide Orius 25EW
Language English Country Czech Republic Media print
Document type Journal Article
Grant support
1/0242/19
Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
1/0166/21
Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
PubMed
37962026
DOI
10.14712/fb2023069010006
PII: fb_2023069010006
Knihovny.cz E-resources
- Keywords
- Bos taurus, DNA methylation, GSTP1, acetamiprid, methylation-specific PCR, tebuconazole,
- MeSH
- Epigenesis, Genetic MeSH
- Glutathione S-Transferase pi * genetics MeSH
- DNA Methylation genetics MeSH
- Pesticides * pharmacology MeSH
- Promoter Regions, Genetic genetics MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glutathione S-Transferase pi * MeSH
- Pesticides * MeSH
DNA methylation, one of the most studied epigenetic mechanisms, when present in the promoter region of genes, causes inhibition of gene expression, and conversely, hypomethylation of these regions enables gene expression. DNA methylation is susceptible to nutritional and environmental influences, and undesirable alterations in methylation patterns manifested in changes in the expression of relevant genes can lead to pathological consequences. In the present work, we studied the methylation status of the bovine GSTP1 gene under the influence of pesticide Mospilan 20SP alone and in combination with pesticide Orius 25EW in in vitro proliferating bovine lymphocytes. We employed methylation-specific PCR, and when studying the effect of pesticide combinations, we also used its real-time version followed by a melting procedure. Our results showed that Mospilan 20SP alone at 5, 25, 50, and 100 µg.ml-1 and 5, 10, 25, and 50 µg.ml-1 for the last 4 and 24 hours of culture with in vitro proliferating bovine lymphocytes, respectively, did not induce methylation of the bovine GSTP1 gene. The same results were revealed when studying the effect of the combination of the pesticides added to the lymphocyte cultures for the last 24 hours of cultivation in the following amounts: 1.25, 2.5, 5, 10, and 25 µg.ml-1 of Mospilan 20SP and 1.5, 3, 6, 15, and 30 µg.ml-1 of Orius 25EW. We have also revealed that the less laborious real-time MSP followed by a melting procedure may replace MSP for studying the methylation status of the GSTP1 gene.
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