In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all S. scabiei within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against S. scabiei were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for S. scabiei was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(S)-HpOTrE (4.624%), nandrolone (4.264%), 1H-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that Urtica fissa contains promising new acaricidal compounds capable of successfully controlling animal mites.

Guizhou Vocational College of Agriculture Qingzhen P R China

Key Laboratory of Animal Disease and Human Health of Sichuan Province College of Veterinary Medicine Sichuan Agricultural University Wenjiang P R China

Qiandongnan Center for Animal Disease Control and Prevention Kaili Guizhou P R China

Zobrazit více v PubMed

Alimi D, Hajri A, Jallouli S, Sebai H. In vitro acaricidal activity of essential oil and crude extracts of Laurus nobilis, (Lauraceae) grown in Tunisia, against arthropod ectoparasites of livestock and poultry: Hyalomma scupense and Dermanyssus gallinae. Vet Parasitol. 2021 Oct;298:109507. PubMed

Alsamri H, Hasasna HE, Baby B, Alneyadi A, Dhaheri YA, Ayoub MA, Eid AH, Vijayan R, Iratni R. Carnosol is a novel inhibitor of p300 acetyltransferase in breast cancer. Front Oncol. 2021 May 13;11:664403. PubMed PMC

Benelli G, Canale A, Toniolo C, Higuchi A, Murugan K, Pavela R, Nicoletti M. Neem (Azadirachta indica): Towards the ideal insecticide? Nat Prod Res. 2017 Feb;31(4):369-86. PubMed

De Melo GO, Muzitano MF, Legora-Machado A, Almeida TA, De Oliveira DB, Kaiser CR, Koatz VL, Costa SS. C-glycosylflavones from the aerial parts of Eleusine indica inhibit LPS-induced mouse lung inflammation. Planta Med. 2005 Apr;71(4):362-3. PubMed

El-Sawy E, Abo-Salem H, Mandour A. 1H-indole-3-carboxaldehyde: Synthesis and reactions. Egypt J Chem. 2017;60(5):723-51.

Gopinath H, Aishwarya M, Karthikeyan K. Tackling scabies: Novel agents for a neglected disease. Int J Dermatol. 2018 Nov;57(11):1293-8. PubMed

Hajhashemi V, Klooshani V. Antinociceptive and anti-inflammatory effects of Urtica dioica leaf extract in animal models. Avicenna J Phytomed. 2013 Spring;3(2):193-200. PubMed PMC

Hao PY, Feng YL, Zhou YS, Song XM, Li HL, Ma Y, Ye CL, Yu XP. Schaftoside interacts with NlCDK1 protein: A mechanism of rice resistance to brown planthopper, Nilaparvata lugens. Front Plant Sci. 2018 May 29;9:710. PubMed PMC

Hu Y, Liao F, Hu Y, Luo B, He Y, Mo Q, Zuo Z, Ren Z, Deng J, Wei Y. Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits. BMC Vet Res. 2014 Dec 20;10(5):970. PubMed PMC

Khan A, Sohaib M, Ullah R, Hussain I, Niaz S, Malak N, de la Fuente J, Khan A, Aguilar-Marcelino L, Alanazi AD, Ben Said M. Structure-based in silico design and in vitro acaricidal activity assessment of Acacia nilotica and Psidium guajava extracts against Sarcoptes scabiei var. cuniculi. Parasitol Res. 2022 Oct;121(10):2901-15. PubMed

Li Y, Wang YL, Li L, Liu L, Lu YX, Cheng XC, Zhang QL. Structural characterization of polysaccharides from the roots of Urtica fissa. J Asian Nat Prod Res. 2009 Nov;11(11):951-7. PubMed

Liao F, Hu Y, Tan H, Wu L, Wang Y, Huang Y, Mo Q, Wei Y. Acaricidal activity of 9-oxo-10,11-dehydroageraphorone extracted from Eupatorium adenophorum in vitro. Exp Parasitol. 2014 May;140:8-11. PubMed

Luo B, Liao F, Hu Y, Liu XI, He Y, Wu L, Tan H, Luo L, Zhou Y, Mo Q, Deng J, Wei Y. Acaricidal activity of extracts from Ligularia virgaurea against the Sarcoptes scabiei mite in vitro. Exp Ther Med. 2015 Jul;10(1):247-50. PubMed PMC

Lynar S, Currie BJ, Baird R. Scabies and mortality. Lancet Infect Dis. 2017 Dec;17(12):1234. PubMed

Mao GN, He YJ, Yang WJ, Hu Y, Hou JL. [Effects of Urtica fissa extract on glycolipid metabolism in type 2 diabetic mice]. Prog Vet Med. 2020;41(1):61-5. Chinese.

Montecino-Latorre D, Cypher BL, Rudd JL, Clifford DL, Mazet JAK, Foley JE. Assessing the role of dens in the spread, establishment and persistence of sarcoptic mange in an endangered canid. Epidemics. 2019 Jun;27:28-40. PubMed

Nong X, Ren YJ, Wang JH, Xie Y, Fang CL, Yang DY, Liu TF, Zhang RH, Chen L, Gu XB, Peng XR, Wang SX, Lai SJ, Yang GY. Clinical efficacy of botanical extracts from Eupatorium adenophorum against the Sarcoptes scabiei (Sarcoptidae: Sarcoptes) in rabbits. Vet Parasitol. 2013 Jul 1;195(1-2):157-64. PubMed

Pasipanodya CN, Tekedza TT, Chatiza FP, Gororo E. Efficacy of neem (Azadirachta indica) aqueous fruit extracts against Sarcoptes scabiei var. suis in grower pigs. Trop Anim Health Prod. 2021 Jan 22;53(1):135. PubMed

Singh SK, Jaiswal AK, Kumari S, Gaur RS, Sharma P, Khushboo R, Jaiswal M. Therapeutic effects of oral fluralaner in pet rabbits with severe sarcoptic mange (Sarcoptes scabiei). Vet Parasitol. 2022 Apr;304:109693. PubMed

Upton R. Stinging nettles leaf (Urtica dioica L.): Extraordinary vegetable medicine. J Herb Med. 2013;3(1):9-38.

Wang M, Feng X, Zhang C, Cheng J, Liu E, Sun L, Li X. The chemical constituents in the decoction of Urtica fissa rhizomes. J Chin Pharm Sci. 2016;25(10):764-70.

Wang MY, Yang Y, Zhou XY, Wang SS, Duan SX, Li XB. The chemical constituents from Urtica fissa leaves. J Asian Nat Prod Res. 2018 Aug;20(8):709-18. PubMed

Wang M, Wu L, Zhang H, Ren DZC, Liu T, Li X. The antioxidant compounds from Urtica fissa flowers. Chem Nat Compd. 2019;55(5):839-42.

Wang CX, Wang ZQ, Li YX, Bai XS, Chen L, Huang XZ, Li YH. [Studies on chemical constituents of Urtica fissa leaves]. Yunnan Minzu Univ. 2022. [cited 2023 May 2]. Available from: https://kns.cnki.net/kcms/detail/53.1192.N.20220615.1326.002.html. Chinese.

Want EJ, Masson P, Michopoulos F, Wilson ID, Theodoridis G, Plumb RS, Shockcor J, Loftus N, Holmes E, Nicholson JK. Global metabolic profiling of animal and human tissues via UPLC-MS. Nat Protoc. 2013 Jan;8(1):17-32. PubMed

Yang L, Sui Y, Zhong L, Ma T, Ma Z, Liu X. Carnosol inhibits the growth and biofilm of Candida albicans. J Mycol Med. 2022 May;32(2):101234. PubMed

Yi Y, Zhang M, Xue H, Yu R, Bao YO, Kuang Y, Chai Y, Ma W, Wang J, Shi X, Li W, Hong W, Li J, Muturi E, Wei H, Wlodarz J, Roszak S, Qiao X, Yang H, Ye M. Schaftoside inhibits 3CLpro and PLpro of SARS-CoV-2 virus and regulates immune response and inflammation of host cells for the treatment of COVID-19. Acta Pharm Sin B. 2022 Nov;12(11):4154-64. PubMed PMC

Zelena E, Dunn WB, Broadhurst D, Francis-McIntyre S, Carroll KM, Begley P, O’Hagan S, Knowles JD, Halsall A; HUSERMET Consortium; Wilson ID, Kell DB. Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum. Anal Chem. 2009 Feb 15;81(4):1357-64. PubMed

Zhang H, Chen Y, Li XB, Deng WX, Wang MY. [Active constituents of Urtica fissa in inhibition of benign prostatic hyperplasia]. Zhongguo Zhong Yao Za Zhi. 2022 Jan;47(2):419-27. Chinese. PubMed

Zhou K, Wu J, Chen J, Zhou Y, Chen X, Wu Q, Xu Y, Tu W, Lou X, Yang G, Jiang S. Schaftoside ameliorates oxygen glucose deprivation-induced inflammation associated with the TLR4/Myd88/Drp1-related mitochondrial fission in BV2 microglia cells. J Pharmacol Sci. 2019a Jan;139(1):15-22. Erratum in: J Pharmacol Sci. 2021 Jul;146(3): 182. PubMed

Zhou Y, Liao F, Weng J, Mo Q, Xu R, Zhang Y, Ren Z, Zhong Z, Zuo Z, Peng G, Deng J, Tang C, Hu Y. Composition and acaricidal activity of essential oil from Elsholtzia densa Benth against Sarcoptes scabiei mites in vitro. Vet Med-Czech. 2019b Apr;64(4):178-83.