SUMMARY: PDBImages is an innovative, open-source Node.js package that harnesses the power of the popular macromolecule structure visualization software Mol*. Designed for use by the scientific community, PDBImages provides a means to generate high-quality images for PDB and AlphaFold DB models. Its unique ability to render and save images directly to files in a browserless mode sets it apart, offering users a streamlined, automated process for macromolecular structure visualization. Here, we detail the implementation of PDBImages, enumerating its diverse image types, and elaborating on its user-friendly setup. This powerful tool opens a new gateway for researchers to visualize, analyse, and share their work, fostering a deeper understanding of bioinformatics. AVAILABILITY AND IMPLEMENTATION: PDBImages is available as an npm package from https://www.npmjs.com/package/pdb-images. The source code is available from https://github.com/PDBeurope/pdb-images.

Biological Data Management and Analysis Core Facility Centre for Structural Biology CEITEC Central European Institute of Technology Masaryk University Brno 62500 Czech Republic

National Centre for Biomolecular Research Faculty of Science Masaryk University Brno 62500 Czech Republic

Protein Data Bank in Europe European Molecular Biology Laboratory European Bioinformatics Institute Wellcome Genome Campus Hinxton Cambridgeshire CB10 1SD United Kingdom

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