Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
38100913
DOI
10.1016/j.scr.2023.103273
PII: S1873-5061(23)00259-3
Knihovny.cz E-zdroje
- MeSH
- Alzheimerova nemoc * genetika metabolismus MeSH
- apolipoprotein E3 genetika MeSH
- buněčná diferenciace MeSH
- genotyp MeSH
- indukované pluripotentní kmenové buňky * metabolismus MeSH
- Krüppel-like faktor 4 MeSH
- leukocyty mononukleární metabolismus MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- apolipoprotein E3 MeSH
- Krüppel-like faktor 4 MeSH
Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from a patient diagnosed with spontaneous late-onset Alzheimer's disease (AD) carrying ApoE3/3 gene and one age-, sex-, and ApoE-matched healthy control. Reprogramming was done using a commercially available Epi5 Reprogramming Kit containing OCT4, SOX2, KLF4, LIN28, and L-MYC as reprogramming factors. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These newly established iPSC lines offer a valuable platform for in vitro modeling of AD.
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