Generation of the Human iPSC Line from Spontaneous Late-Onset Alzheimer's Disease Patient with ApoE3/3 Genotype and Sex-, Age-, and ApoE-Matched Healthy Control
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
38100913
DOI
10.1016/j.scr.2023.103273
PII: S1873-5061(23)00259-3
Knihovny.cz E-resources
- MeSH
- Alzheimer Disease * genetics metabolism MeSH
- Apolipoprotein E3 genetics MeSH
- Cell Differentiation MeSH
- Genotype MeSH
- Induced Pluripotent Stem Cells * metabolism MeSH
- Kruppel-Like Factor 4 MeSH
- Leukocytes, Mononuclear metabolism MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Apolipoprotein E3 MeSH
- Kruppel-Like Factor 4 MeSH
Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from a patient diagnosed with spontaneous late-onset Alzheimer's disease (AD) carrying ApoE3/3 gene and one age-, sex-, and ApoE-matched healthy control. Reprogramming was done using a commercially available Epi5 Reprogramming Kit containing OCT4, SOX2, KLF4, LIN28, and L-MYC as reprogramming factors. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These newly established iPSC lines offer a valuable platform for in vitro modeling of AD.
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