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Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export

Ying, Wei
Author Ying, Wei ORCID Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China
Wang, Yaowei
Author Wang, Yaowei ORCID Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
Wei, Hong
Author Wei, Hong ORCID Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China
Luo, Yongming
Author Luo, Yongming ORCID Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
Ma, Qian
Author Ma, Qian ORCID Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
Zhu, Heyuan
Author Zhu, Heyuan Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
Janssens, Hilde
Author Janssens, Hilde ORCID Department of Organic and Macromolecular Chemistry, Ghent University, 9000 Ghent, Belgium
Vukašinović, Nemanja
Author Vukašinović, Nemanja ORCID Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
Kvasnica, Miroslav
Author Kvasnica, Miroslav ORCID Laboratory of Growth Regulators, Institute of Experimental Botany, The Czech Academy of Sciences and Palacký University, 77900 Olomouc, Czech Republic
Winne, Johan M
Author Winne, Johan M ORCID Department of Organic and Macromolecular Chemistry, Ghent University, 9000 Ghent, Belgium
Gao, Yongxiang
Author Gao, Yongxiang ORCID Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China
Tan, Shutang
Author Tan, Shutang ORCID Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China
Friml, Jiří
Author Friml, Jiří ORCID Institute of Science and Technology Austria (ISTA), 3400 Klosterneuburg, Austria
Liu, Xin
Author Liu, Xin ORCID Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China
Russinova, Eugenia
Author Russinova, Eugenia ORCID Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
Sun, Linfeng
Author Sun, Linfeng ORCID Department of Neurology of The First Affiliated Hospital of USTC, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei National Research Center for Physical Sciences at the Microscale, Biomedical Sciences and Health Laboratory of Anhui Province, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China

Science (New York, N.Y.). 2024 Mar 22 ; 383 (6689) : eadj4591. [epub] 20240322

Science
ISSN 1095-9203 | 0036-8075
Source

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid38513023

PubMed 38513023
DOI 10.1126/science.adj4591
Knihovny.cz E-resources

MeSH
ATP-Binding Cassette Transporters * chemistry genetics metabolism MeSH
Adenosine Triphosphate metabolism MeSH
Arabidopsis * genetics metabolism MeSH
Brassinosteroids * metabolism MeSH
Protein Conformation MeSH
Indoleacetic Acids metabolism MeSH
Arabidopsis Proteins * chemistry genetics metabolism MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
ATP-Binding Cassette Transporters * MeSH
ABCB19 protein, Arabidopsis MeSH Browser
Adenosine Triphosphate MeSH
Brassinosteroids * MeSH
Indoleacetic Acids MeSH
Arabidopsis Proteins * MeSH

Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.

Center for Plant Systems Biology VIB 9052 Ghent Belgium

Department of Neurology of The 1st Affiliated Hospital of USTC MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics Hefei National Research Center for Physical Sciences at the Microscale Biomedical Sciences and Health Laboratory of Anhui Province Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230027 China

Department of Organic and Macromolecular Chemistry Ghent University 9000 Ghent Belgium

Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Ghent Belgium

Institute of Science and Technology Austria 3400 Klosterneuburg Austria

Laboratory of Growth Regulators Institute of Experimental Botany The Czech Academy of Sciences and Palacký University 77900 Olomouc Czech Republic

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