The study aimed to evaluate and compare the routes and rates of the depletion of the antiparasitic praziquantel (PZQ), a derivative of pyrazinoisoquinoline, following its oral administration in grass carp (Ctenopharyngodon idella). We focused on the depletion of PZQ and its major metabolites - cis-hydroxy praziquantel (CPZQ) and trans-hydroxy praziquantel (TPZQ), in water, the plasma, hepatopancreas, kidney, muscle, and skin, following a single oral administration of PZQ in a concentration of 50 mg/kg. Fish were sampled before the drug administration and then eight times in the course of the 30-day-long experiment. Our results indicate the rapid absorption and elimination of PZQ and its metabolites in all the analysed matrices. The most PZQ-burdened tissue was the hepatopancreas, the gill and the skin. In all the samples, the concentration of the drug and its metabolites consistently declined over time. The residue of the parent compound was detected for the longest time in all the tissues. During the study, a significant (P < 0.01) correlation was found within the concentration of PZQ, CPZQ, and TPZQ in the water and all the biological matrices. It was also found that the PZQ residue was not detected below the maximum residue levels (i.e., 20 μg/kg) until 16 days after exposure in the muscle and skin.

Department of Animal Husbandry Animal Nutrition and Biochemistry Faculty of Veterinary Hygiene and Ecology University of Veterinary Sciences Brno Brno Czech Republic

Department of Animal Protection and Welfare and Veterinary Public Health Faculty of Veterinary Hygiene and Ecology University of Veterinary Sciences Brno Brno Czech Republic

South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Research Institute of Fish Culture and Hydrobiology Faculty of Fisheries and Protection of Waters University of South Bohemia in Ceske Budejovice Vodnany Czech Republic

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Bader C, Starling DE, Jones DE, Brewer MT. Use of praziquantel to control platyhelminth parasites of fish. J Vet Pharmacol Ther. 2019 Mar;42(2):139-53. PubMed

Baralla E, Varoni MV, Nieddu M, Demontis MP, Merella P, Burreddu C, Garippa G, Boatto G. Determination of praziquantel in Sparus aurata L. after administration of medicated animal feed. Animals (Basel). 2020 Mar 21;10(3):528. PubMed PMC

Hirazawa N, Akiyama K, Umeda N. Differences in sensitivity to the anthelmintic praziquantel by the skin-parasitic monogeneans Benedenia seriolae and Neobenedenia girellae. Aquac. 2013 Aug 10;404-405:59-64.

EMA – Science Medicines Health. MRL summary opinion [Internet]. Amsterdam, the Netherlands: European Medicines Agency. 2022. [cited 2022 Sep 9]. Available from: https://www.ema.europa.eu/en/documents/mrl-summary/praziquantel-all-ruminants-except-bovine-equidae-fin-fish-summary-opinion-cvmp-establishment-maximum_en.pdf.

Kim CS, Cho JB, Ahn KJ, Lee JI, Kim KH. Depletion of praziquantel in muscle tissue and skin of cultured rockfish (Sebastes schlegeli) under the commercial culture conditions. Aquac. 2003 Apr;219(1-4):1-7.

Kogiannou D, Nikoloudaki C, Valsamidi V, Kotsiri M, Golomazou E, Rigos G. Pharmacokinetics of praziquantel in gilthead seabream (Sparus aurata) plasma and gills. Aquac. 2023 Aug 5;557:739975.

Kogiannou D, Rigos G. Praziquantel depletion from muscle plus skin tissue of gilthead sea bream (Sparus aurata). Mediterr Mar Sci. 2021 Feb 22;22(1):121-4.

Kolarova J, Stara A, Zuskova E, Velisek J. Safety of the anthelmintic drugs levamisole, fenbendazole, and ivermectin administered in therapeutic bath for the common carp Cyprinus carpio. Vet Med-Czech. 2022 Apr 24;67(7):371-8. PubMed PMC

Meister I, Leonidova A, Kovac J, Duthaler U, Keiser J, Huwyler J. Development and validation of an enantioselective LC-MS/MS method for the analysis of the anthelmintic drug praziquantel and its main metabolite in human plasma, blood and dried blood spots. J Pharm Biomed. 2016 Jan 25;118:81-8. PubMed

Noga EJ. Fish disease: Diagnosis and treatment. Ames, USA: Willey-Blackwell Publishing, Inc.; 2010. 519 p.

RUMA guidelines. Responsible use of anti-parasitics in aquaculture [Internet]. 2010. [cit. 2021 Jul 12]. Available from: www.ruma.org.uk/wp-content/uploads/2014/09/Anti-parasitics-in-aquaculture-Final-4-May-2010-_3_.pdf.

Soukupova-Markova Z, Doubkova V, Marsalek P, Svobodova Z, Papezikova I, Lang S, Navratil S, Palikova M. Degradation rate of praziquantel and fenbendazole in rainbow trout following oral administration. Neuro Endocrinol Lett. 2015;36(S1):64-7. PubMed

Staudt U, Schmahl G, Blaschke G, Mehlhorn H. Light and scanning electron microscopy studies on the effects of the enantiomers of praziquantel and its main metabolite on Schistosoma mansoni in vitro. Parasitol Res. 1992;78(5):392-7. PubMed

Velisek J, Zuskova E, Kubec J, Sandova M, Stara A. Effects of praziquantel on common carp embryos and larvae. Sci Rep. 2022 Oct 14;12:17290. PubMed PMC

Xie X, Zhao Y, Yang X, Hu K. Comparison of praziquantel pharmacokinetics and tissue distribution in fresh and brackish water cultured grass carp (Ctenopharyngodon idellus) after oral administration of single bolus. BMC Vet Res. 2015 Apr 1;11:84. PubMed PMC

Xu N, Dong J, Yang Y, Ai X. Pharmacokinetics and residue depletion of praziquantel in rice field eels Monopterus albus. Dis Aquat Org. 2016 Apr;119(1):67-74. PubMed

Zuskova E, Piackova V, Valentova O, Zalohova K, Velisek J. Acute toxicity of praziquantel to fish Danio rerio and planktonic crustacean Daphnia magna. Vet Med-Czech. 2022 Oct 24;67(11):579-84. PubMed PMC