Two cases with discrepancy in the quantitative cytological assessment of cerebrospinal fluid in neonatal samples using light microscopy in comparison with Sysmex XN-1000
Jazyk angličtina Země Chorvatsko Médium print-electronic
Typ dokumentu kazuistiky, srovnávací studie, časopisecké články
PubMed
38665873
PubMed Central
PMC11042561
DOI
10.11613/bm.2024.020802
PII: bm-34-2-020802
Knihovny.cz E-zdroje
- Klíčová slova
- biochemistry, cerebrospinal fluid, cytology, hematology, neonatology,
- MeSH
- leukocyty mononukleární patologie cytologie MeSH
- lidé MeSH
- mikroskopie * metody MeSH
- mozkomíšní mok cytologie MeSH
- neutrofily cytologie patologie MeSH
- novorozenec MeSH
- počet leukocytů MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- srovnávací studie MeSH
We present two cases from the neonatal department with cerebrospinal fluid examination. We revealed a striking discrepancy in polymorphonuclear (PMN) and mononuclear (MN) cell counts using conventional light microscopy in comparison with automated analyzer Sysmex XN-1000 (PMNs - 13 vs. 173x106/L, MNs - 200 vs. 67x106/L in case 1 and PMNs - 13 vs. 372x106/L, MNs - 411 vs. 179x106/L in case 2). We revealed the dominant presence of hemosiderophages in both cases in cytospin slide. Even though Sysmex XN-1000 offers fast examination with a low sample volume, there is possibility of misdiagnosis, with negative impact on the patient.
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