In this study, we conducted an extensive investigation of the biodegradation capabilities and stress response of the newly isolated strain Pseudomonas veronii SM-20 in order, to assess its potential for bioremediation of sites contaminated with polycyclic aromatic hydrocarbons (PAHs). Initially, phenotype microarray technology demonstrated the strain's proficiency in utilizing various carbon sources and its resistance to certain stressors. Genomic analysis has identified numerous genes involved in aromatic hydrocarbon metabolism. Biodegradation assay analyzed the depletion of phenanthrene (PHE) when it was added as a sole carbon and energy source. We found that P. veronii strain SM-20 degraded approximately 25% of PHE over a 30-day period, starting with an initial concentration of 600 µg/mL, while being utilized for growth. The degradation process involved PHE oxidation to an unstable arene oxide and 9,10-phenanthrenequinone, followed by ring-cleavage. Comparative proteomics provided a comprehensive understanding of how the entire proteome responded to PHE exposure, revealing the strain's adaptation in terms of aromatic metabolism, surface properties, and defense mechanism. In conclusion, our findings shed light on the promising attributes of P. veronii SM-20 and offer valuable insights for the use of P. veronii species in environmental restoration efforts targeting PAH-impacted sites.

Biomedical Research Center Slovak Academy of Sciences Dubravska c 9 845 05 Bratislava Slovakia

Department of Agriculture Food Environment and Forestry University of Florence 50100 Firenze Italy

Department of Pharmacy and Biotechnology University of Bologna 40126 Bologna Italy

Faculty of Pharmacy Comenius University Odbojarov 10 832 32 Bratislava Slovakia

Faculty of Science Charles University Vinicna 5 12844 Prague Czech Republic

Institute of Microbiology Czech Academy of Sciences Videnska 1083 14220 Prague Czech Republic

Military Health Institute Military Medical Agency U Vojenske Nemocnice 1200 16902 Prague Czech Republic

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