The clinical consequences of toxoplasmosis are greatly dependent on the Toxoplasma gondii strain causing the infection. To better understand its epidemiology and design appropriate control strategies, it is important to determine the strain present in infected animals. Serotyping methods are based on the detection of antibodies that react against segments of antigenic proteins presenting strain-specific polymorphic variations, offering a cost-effective, sensitive, and non-invasive alternative to genotyping techniques. Herein, we evaluated the applicability of a panel of peptides previously characterized in mice and humans to serotype sheep and pigs. To this end, we used 51 serum samples from experimentally infected ewes (32 type II and 19 type III), 20 sheep samples from naturally infected sheep where the causative strain was genotyped (18 type II and 2 type III), and 40 serum samples from experimentally infected pigs (22 type II and 18 type III). Our ELISA test results showed that a combination of GRA peptide homologous pairs can discriminate infections caused by type II and III strains of T. gondii in sheep and pigs. Namely, the GRA3-I/III-43 vs. GRA3-II-43, GRA6-I/III-213 vs. GRA6-II-214 and GRA6-III-44 vs. GRA6-II-44 ratios showed a statistically significant predominance of the respective strain-type peptide in sheep, while in pigs, in addition to these three peptide pairs, GRA7-II-224 vs. GRA7-III-224 also showed promising results. Notably, the GRA6-44 pair, which was previously deemed inefficient in mice and humans, showed a high prediction capacity, especially in sheep. By contrast, GRA5-38 peptides failed to correctly predict the strain type in most sheep and pig samples, underpinning the notion that individual standardization is needed for each animal species. Finally, we recommend analyzing for each animal at least 2 samples taken at different time points to confirm the obtained results.

Central European Institute of Technology University of Veterinary Sciences Brno Czechia

Faculty of Veterinary Medicine University of Veterinary Sciences Brno Czechia

Laboratory of Apicomplexan Biology Institut Pasteur de Montevideo Montevideo Uruguay

SALUVET group Faculty of Veterinary Sciences Animal Health Department Complutense University of Madrid Madrid Spain

Veterinary Research Institute Brno Czechia

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Aguirre A. A., Longcore T., Barbieri M., Dabritz H., Hill D., Klein P. N., et al. (2019). The one health approach to toxoplasmosis: epidemiology, control, and prevention strategies. Ecohealth 16, 378–390. doi:  10.1007/s10393-019-01405-7 PubMed DOI PMC

Almeria S., Dubey J. P. (2021). Foodborne transmission of PubMed DOI

Arranz-Solís D., Carvalheiro C. G., Zhang E. R., Grigg M. E., Saeij J. P. J. (2021). PubMed DOI PMC

Arranz-Solís D., Cordeiro C., Young L. H., Dardé M. L., Commodaro A. G., Grigg M. E., et al. (2019). Serotyping of PubMed DOI PMC

Belluco S., Mancin M., Conficoni D., Simonato G., Pietrobelli M., Ricci A. (2016). Investigating the determinants of PubMed DOI PMC

Belluco S., Simonato G., Mancin M., Pietrobelli M., Ricci A. (2018). PubMed DOI

Castaño P., Fuertes M., Ferre I., Fernández M., Ferreras M., del C., et al. (2014). Placental thrombosis in acute phase abortions during experimental PubMed DOI PMC

Chaichan P., Mercier A., Galal L., Mahittikorn A., Ariey F., Morand S., et al. (2017). Geographical distribution of PubMed DOI

Dard C., Fricker-Hidalgo H., Brenier-Pinchart M. P., Pelloux H. (2016). Relevance of and new developments in serology for toxoplasmosis. Trends Parasitol. 32, 492–506. doi:  10.1016/j.pt.2016.04.001 PubMed DOI

Dubey J. P., Vianna M. C. B., Sousa S., Canada N., Meireles S., Costa J. M. C., et al. (2006). Characterization of PubMed DOI

Fernández-Escobar M., Calero-Bernal R., Benavides J., Regidor-Cerrillo J., Guerrero-Molina M. C., Gutiérrez-Expósito D., et al. (2020). Isolation and genetic characterization of PubMed DOI PMC

Fernández-Escobar M., Schares G., Maksimov P., Joeres M., Ortega-Mora L. M., Calero-Bernal R. (2022). PubMed DOI PMC

Galal L., Ajzenberg D., Hamidović A., Durieux M.-F., Dardé M.-L., Mercier A. (2018). PubMed DOI

Hamilton C. M., Black L., Oliveira S., Burrells A., Bartley P. M., Melo R. P. B., et al. (2019). Comparative virulence of Caribbean, Brazilian and European isolates of PubMed DOI PMC

Joeres M., Maksimov P., Höper D., Calvelage S., Calero-Bernal R., Fernández-Escobar M., et al. (2024). Genotyping of European PubMed DOI PMC

Khan A., Dubey J. P., Su C., Ajioka J. W., Rosenthal B. M., Sibley L. D. (2011). Genetic analyses of atypical PubMed DOI PMC

Kong J., Grigg M. E., Uyetake L., Parmley S., Boothroyd J. C. (2003). Serotyping of PubMed DOI

Largo-de la Torre A., Diezma-Díaz C., Calero-Bernal R., Atencia-Cibreiro G., Sánchez-Sánchez R., Ferre I., et al. (2022). Archetypal type II and III PubMed DOI PMC

López-Ureña N. M., Calero-Bernal R., González-Fernández N., Blaga R., Koudela B., Ortega-Mora L. M., et al. (2023). Optimization of the most widely used serological tests for a harmonized diagnosis of PubMed DOI

Lorenzi H., Khan A., Behnke M. S., Namasivayam S., Swapna L. S., Hadjithomas M., et al. (2016). Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic PubMed DOI PMC

Maksimov P., Basso W., Zerweck J., Schutkowski M., Reimer U., Maksimov A., et al. (2018). Analysis of PubMed DOI

Maksimov P., Zerweck J., Dubey J. P., Pantchev N., Frey C. F., Maksimov A., et al. (2013). Serotyping of PubMed DOI PMC

Maksimov P., Zerweck J., Maksimov A., Hotop A., Groß U., Pleyer U., et al. (2012. a). Peptide microarray analysis of in silico -predicted epitopes for serological diagnosis of PubMed DOI PMC

Maksimov P., Zerweck J., Maksimov A., Hotop A., Groß U., Spekker K., et al. (2012. b). Analysis of clonal type-specific antibody reactions in PubMed DOI PMC

McLeod R., Boyer K. M., Lee D., Mui E., Wroblewski K., Karrison T., et al. (2012). Prematurity and severity are associated with toxoplasma gondii alleles (NCCCTS 1981-2009). Clin. Infect. Dis. 54, 1595–1605. doi:  10.1093/cid/cis258 PubMed DOI PMC

Mukhopadhyay D., Arranz-Solís D., Saeij J. P. J. (2020). Influence of the host and parasite strain on the immune response during PubMed DOI PMC

Peyron F., Lobry J. R., Musset K., Ferrandiz J., Gomez-Marin J. E., Petersen E., et al. (2006). Serotyping of PubMed DOI

Sánchez-Sánchez R., Ferre I., Regidor-Cerrillo J., Gutiérrez-Expósito D., Ferrer L. M., Arteche-Villasol N., et al. (2019). Virulence in mice of a PubMed DOI PMC

Sánchez-Sánchez R., Imhof D., Hecker Y. P., Ferre I., Re M., Moreno-Gonzalo J., et al. (2023). An early treatment with BKI-1748 exhibits full protection against abortion and congenital infection in sheep experimentally infected with PubMed DOI PMC

Shwab E. K., Jiang T., Pena H. F. J., Gennari S. M., Dubey J. P., Su C. (2016). The ROP18 and ROP5 gene allele types are highly predictive of virulence in mice across globally distributed strains of PubMed DOI

Sousa S., Ajzenberg D., Canada N., Freire L., da J. M. C., Dardé M. L., et al. (2006). Biologic and molecular characterization of PubMed DOI

Sousa S., Ajzenberg D., Marle M., Aubert D., Villena I., Da Costa J. C., et al. (2009). Selection of polymorphic peptides from GRA6 and GRA7 sequences of PubMed DOI PMC

Sousa S., Ajzenberg D., Vilanova M., Costa J., Darde M. L. (2008). Use of GRA6-derived synthetic polymorphic peptides in an immunoenzymatic assay to serotype PubMed DOI PMC

Sousa S., Canada N., Correia da Costa J. M., Dardé M.-L. L. (2010). Serotyping of naturally Toxoplasma gondii infected meat-producing animals. Veterinary Parasitol. 169, 24–28. doi:  10.1016/j.vetpar.2009.12.025 PubMed DOI

Sousa S., Fernandes M., Correia da Costa J. M. (2023). Serotyping, a challenging approach for PubMed DOI PMC

Stelzer S., Basso W., Benavides Silván J., Ortega-Mora L. M., Maksimov P., Gethmann J., et al. (2019). PubMed DOI PMC

Su C., Dubey J. P., Ajzenberg D., Khan A., Ajioka J. W., Rosenthal B. M., et al. (2012). Globally diverse PubMed DOI PMC

Vallejo R., Benavides J., Arteche-Villasol N., Sánchez-Sánchez R., Calero-Bernal R., Ferreras M. C., et al. (2023). Experimental infection of sheep at mid-pregnancy with archetypal type II and type III PubMed DOI