Modulation of response to braconid wasp venom by adipokinetic hormone in Drosophila melanogaster
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
39154974
DOI
10.1016/j.cbpc.2024.110005
PII: S1532-0456(24)00173-X
Knihovny.cz E-resources
- Keywords
- Adipokinetic hormone, Habrobracon venom, Immune responsive genes, Muscle structure, Oxidative stress,
- MeSH
- Central Nervous System drug effects MeSH
- Drosophila melanogaster * drug effects MeSH
- Insect Hormones * metabolism MeSH
- Catalase metabolism genetics MeSH
- Pyrrolidonecarboxylic Acid * analogs & derivatives MeSH
- Oligopeptides * pharmacology toxicity MeSH
- Drosophila Proteins * metabolism genetics MeSH
- Wasps * drug effects MeSH
- Superoxide Dismutase metabolism MeSH
- Wasp Venoms * toxicity MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- adipokinetic hormone MeSH Browser
- Insect Hormones * MeSH
- Catalase MeSH
- Pyrrolidonecarboxylic Acid * MeSH
- Oligopeptides * MeSH
- Drosophila Proteins * MeSH
- Superoxide Dismutase MeSH
- Wasp Venoms * MeSH
The minute wasp Habrobracon hebetor venom (HH venom) is a potent cocktail of toxins that paralyzes the victim's muscles and suppresses humoral and cellular immunity. This study examined the effect of HH venom on specific biochemical, physiological, and ultrastructural characteristics of the thoracic and nervous (CNS) tissues of Drosophila melanogaster under in vitro conditions. Venom treatment modulated the activities of superoxide dismutase (SOD) and catalase (CAT), endogenous Drome-AKH level, and affected the relative viability of the cells. Additionally, it reduced the expression of genes related to the immune system in the CNS, including Keap1, Relish, Nox, Eiger, Gadd45, and Domeless, as well as in the thoracic muscles, except for Nox. Besides, venom treatment led to deteriorative changes in the ultrastructure of muscle cells, particularly affecting the mitochondria. When venom and Drosophila melanogaster-adipokinetic hormone (Drome-AKH) were applied together, the effects of the venom alone were often modulated. The harmful effect of the venom on SOD activity was relatively reduced and the activity returned to a level similar to that of the control. In the CNS, the simultaneous application of venom and hormones abolished the suppression of previously reported immune-related genes (except for Gadd45), whereas in the muscles, this was only true for Eiger. Additionally, Drome-AKH restored cell structure to a level comparable to that of the control and lessened the harmful effects of HH venom on muscle mitochondria. These findings suggest a general body response of D. melanogaster to HH venom and a partial defensive role of Drome-AKH in this process.
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