Reversed-Phase Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Method for High-Throughput Lipidomic Quantitation
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
- Klíčová slova
- High-throughput lipidomics, Mass spectrometry, Plasma, Quantitation, Reversed-phase, Serum, Ultrahigh-performance liquid chromatography,
- MeSH
- chromatografie s reverzní fází * metody MeSH
- hmotnostní spektrometrie metody MeSH
- kapalinová chromatografie-hmotnostní spektrometrie MeSH
- lidé MeSH
- lipidomika * metody MeSH
- lipidy * analýza MeSH
- rychlé screeningové testy metody MeSH
- software MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- lipidy * MeSH
Reversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method is optimized for the quantitation of a large number of lipid species in biological samples, primarily in human plasma and serum. The method uses a C18 bridged ethylene hybrid (BEH) column (150 × 2.1 mm; 1.7 μm) for the separation of lipids from 23 subclasses with a total run time of 25 min. Lipid species separation allows the resolution of isobaric and isomeric lipid forms. A triple quadrupole mass spectrometer is used for targeted lipidomic analysis using multiple reaction monitoring (MRM) in the positive ion mode. Data are evaluated by Skyline software, and the concentrations of analytes are determined using internal standards per each individual lipid class.
Zobrazit více v PubMed
Vaňková Z, Peterka O, Chocholoušková M et al (2022) Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS. Anal Bioanal Chem 414:319–331. https://doi.org/10.1007/s00216-021-03492-4 PubMed DOI
Huynh K, Barlow CK, Jayawardana KS et al (2019) High-Throughput Plasma Lipidomics: detailed mapping of the associations with cardiometabolic risk factors. Cell Chem Biol 26:71–84. https://doi.org/10.1016/j.chembiol.2018.10.008 PubMed DOI
Lísa M, Netušilová K, Franěk L et al (2011) Characterization of fatty acid and triacylglycerol composition in animal fats using silver-ion and non-aqueous reversed-phase high-performance liquid chromatography/mass spectrometry and gas chromatography/flame ionization detection. J Chromatogr A 1218:7499–7510. https://doi.org/10.1016/j.chroma.2011.07.032 PubMed DOI
Holčapek M, Liebisch G, Ekroos K (2018) Lipidomic analysis. Anal Chem 90:4249–4257. https://doi.org/10.1021/acs.analchem.7b0539 PubMed DOI
Wolrab D, Chocholoušková M, Jirásko R et al (2020) Validation of lipidomic quantitative methods based on lipid class separation – mass spectrometry: comparison of supercritical fluid chromatography and hydrophilic interaction liquid chromatography. Anal Bioanal Chem 412:2375–2388. https://doi.org/10.1007/s00216-020-02473-3 PubMed DOI
MacLean B, Tomazela DM, Shulman N et al (2010) Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26:966–968. https://doi.org/10.1093/bioinformatics/btq054 PubMed DOI PMC
