The precise measurement of cell temperature and an in-depth understanding of thermogenic processes are critical in unraveling the complexities of cellular metabolism and its implications for health and disease. This review focuses on the mechanisms of local temperature generation within cells and the array of methods developed for accurate temperature assessment. The contact and noncontact techniques are introduced, including infrared thermography, fluorescence thermometry, and other innovative approaches to localized temperature measurement. The role of thermogenesis in cellular metabolism, highlighting the integral function of temperature regulation in cellular processes, environmental adaptation, and the implications of thermogenic dysregulation in diseases such as metabolic disorders and cancer are further discussed. The challenges and limitations in this field are critically analyzed while technological advancements and future directions are proposed to overcome these barriers. This review aims to provide a consolidated resource for current methodologies, stimulate discussion on the limitations and challenges, and inspire future innovations in the study of cellular thermodynamics.

Department of Laboratory Medicine Faculty of Health Care and Social Work University of Trnava in Trnava Universitne namestie 1 Trnava 918 43 Slovakia

Department of Microelectronics The Faculty of Electrical Engineering and Communication Technology Brno University of Technology Technická 3058 10 Brno 616 00 Czech Republic

Institute of Biology and Medical Genetics 1st Faculty of Medicine Charles University and General University Hospital Prague Albertov 4 Prague 128 00 Czech Republic

School of Mechanical Engineering Northwestern Polytechnical University Xi'an Shaanxi 710072 P R China

Zobrazit více v PubMed

Bicego K. C., Barros R. C., Branco L. G., Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2007, 147, 616. PubMed

Cohen P., Kajimura S., Nat. Rev. Mol. Cell Biol. 2021, 22, 393. PubMed PMC

a) Beignon F., Gueguen N., Tricoire‐Leignel H., Mattei C., Lenaers G., Cell. Mol. Life Sci. 2022, 79, 525; PubMed PMC

b) Yang X., Heinemann M., Howard J., Huber G., Iyer‐Biswas S., Treut G. Le, Lynch M., Montooth K. L., Needleman D. J., Pigolotti S., Proc. Natl. Acad. Sci. U.S.A. 2021, 118, e2026786118. PubMed PMC

Hartl F. U., Annu. Rev. Biochem. 2016, 85, 1. PubMed

Guo R., Gu J., Zong S., Wu M., Yang M., Biomed. J. 2018, 41, 9. PubMed PMC

Lazcano A., Miller S. L., J. Mol. Evol. 1999, 49, 424. PubMed

Smith N. P., Barclay C. J., Loiselle D. S., Progr. Biophys. Molr. Biol. 2005, 88, 1. PubMed

Baffou G., Rigneault H., Marguet D., Jullien L., Nat. Methods 2014, 11, 899. PubMed

Bai T., Gu N., Small 2016, 12, 4590. PubMed

Lahiri B. B., Bagavathiappan S., Jayakumar T., Philip J., Infrared Phys. Technol. 2012, 55, 221. PubMed PMC

Sun Y., Fu M., Bian M., Zhu Q., Biotechnol. Bioeng. 2023, 120, 7. PubMed

Inada N., Fukuda N., Hayashi T., Uchiyama S., Nat. Protoc. 2019, 14, 1293. PubMed

Shaebani M. R., Wysocki A., Winkler R. G., Gompper G., Rieger H., Nat. Rev. Phys. 2020, 2, 181.

Kruglov A. G., Romshin A. M., Nikiforova A. B., Plotnikova A., Vlasov I. I., Int. J. Mol. Sci. 2023, 24, 16955. PubMed PMC

Qiao J., Chen C., Shangguan D., Mu X., Wang S., Jiang L., Qi L., Anal. Chem. 2018, 90, 12553. PubMed

Jorgensen P. L., Pedersen P. A., Biochim. Biophys. Acta Bioenerg. 2001, 1505, 57. PubMed

Qiao J., Ma Q., Song Y., Qi L., ACS Appl. Bio Mater. 2022, 5, 5826. PubMed

Nowack J., Giroud S., Arnold W., Ruf T., Front. Physiol. 2017, 8, 889. PubMed PMC

Qiao J., Hwang Y.‐H., Chen C.‐F., Qi L., Dong P., Mu X.‐Y., Kim D.‐P., Anal. Chem. 2015, 87, 10535. PubMed

Qiao J., Hwang Y.‐H., Kim D.‐P., Qi L., Anal. Chem. 2020, 92, 8579. PubMed

Hohendanner F., McCulloch A. D., Blatter L. A., Michailova A. P., Front. Pharmacol. 2014, 5, 35. PubMed PMC

a) Fahimi P., Matta C. F., Trends Chem. 2022, 4, 96;

b) Macherel D., Haraux F., Guillou H., Bourgeois O., Biochim. Biophys. Acta Bioenerg. 2021, 1862, 148348. PubMed

a) Arai S., Lee S.‐C., Zhai D., Suzuki M., Chang Y. T., Sci. Rep. 2014, 4, 6701; PubMed PMC

b) Liu X., Yamazaki T., Kwon H.‐Y., Arai S., Chang Y.‐T., Mater. Today Bio. 2022, 16, 100405. PubMed PMC

Okabe K., Inada N., Gota C., Harada Y., Funatsu T., Uchiyama S., Nat. Commun. 2012, 3, 705. PubMed PMC

a) Lynch M., Marinov G. K., Proc. Natl. Acad. Sci. U.S.A. 2015, 112, 15690; PubMed PMC

b) Mathews C. K., FASEB J. 2014, 28, 3832. PubMed PMC

Chuma S., Kiyosue K., Akiyama T., Kinoshita M., Shimazaki Y., Uchiyama S., Sotoma S., Okabe K., Harada Y., Nat. Commun. 2024, 15, 3473. PubMed PMC

Sotoma S., Zhong C., Kah J. C. Y., Yamashita H., Plakhotnik T., Harada Y., Suzuki M., Sci. Adv. 2021, 7, eabd7888. PubMed PMC

Okabe K., Uchiyama S., Commun. Biol. 2021, 4, 1377. PubMed PMC

Qiao J., Mu X., Qi L., Biosens. Bioelectron. 2016, 85, 403. PubMed

Hannon G., Tansi F. L., Hilger I., Prina‐Mello A., Adv. Therapeut. 2021, 4, 2000267.

Viallard C., Larrivée B., Angiogenesis 2017, 20, 409. PubMed

Horsman M. R., Int. J. Hyperth. 2006, 22, 197. PubMed

Tan L., Chen S., Wei G., Li Y., Liao J., Jin H., Zou Y., Huang M., Peng Z., Guo Y., Cancer Lett. 2019, 460, 29. PubMed

Song C., Park H., Lee C. K., Griffin R., Int. J. Hyperth. 2005, 21, 761. PubMed

Vancsik T., Kovago C., Kiss E., Papp E., Forika G., Benyo Z., Meggyeshazi N., Krenacs T., J. Cancer 2018, 9, 41. PubMed PMC

Li T., Jiang S., Zhang Y., Luo J., Li M., Ke H., Deng Y., Yang T., Sun X., Chen H., Nat. Commun. 2023, 14, 2498. PubMed PMC

Lepock J., Int. J. Hyperth. 2004, 20, 115.

Nikanorova A. A., Barashkov N. A., Pshennikova V. G., Gotovtsev N. N., Romanov G. P., Solovyev A. V., Kuzmina S. S., Sazonov N. N., Fedorova S. A., Genes 2022, 13, 1612. PubMed PMC

Bianco A. C., Kieffer J. D., Silva J. E., Endocrinology 1992, 130, 2625. PubMed

Scheele C., Nielsen S., Redox Biol. 2017, 12, 770. PubMed PMC

Yahara T., Koga T., Yoshida S., Nakagawa S., Deguchi H., Shirouzu K., Surg. Today 2003, 33, 243. PubMed

Knapp J. P., Kakish J. E., Bridle B. W., Speicher D. J., Biomedicines 2022, 10, 2024. PubMed PMC

Komatsu S., Tago M., Ando Y., Asoh T.‐A., Kikuchi A., J. Control. Rel. 2021, 331, 1. PubMed

Cypess A. M., Lehman S., Williams G., Tal I., Rodman D., Goldfine A. B., Kuo F. C., Palmer E. L., Tseng Y.‐H., Doria A., N. Engl. J. Med. 2009, 360, 1509. PubMed PMC

Gallagher D., Visser M., Sepulveda D., Pierson R. N., Harris T., Heymsfield S. B., Am. J. Epidemiol. 1996, 143, 228. PubMed

Nedergaard J., Bengtsson T., Cannon B., Am. J. Physiol.‐Endocrinol. Metabol. 2007, 293, E444. PubMed

Young P., Wilson S., Arch J., Life Sci. 1984, 34, 1111. PubMed

Qiao J., Wu D., Song Y., Ji W., Yue Q., Mao L., Qi L., Anal. Chem. 2021, 93, 14743. PubMed

a) Blondin D. P., Nielsen S., Kuipers E. N., Severinsen M. C., Jensen V. H., Miard S., Jespersen N. Z., Kooijman S., Boon M. R., Fortin M., Cell Metab. 2020, 32, 287; PubMed

b) Straat M. E., Hoekx C. A., van Velden F. H., Arias‐Bouda L. M. P., Dumont L., Blondin D. P., Boon M. R., Martinez‐Tellez B., Rensen P. C., Cell Rep. Med. 2023, 4. PubMed PMC

Riis‐Vestergaard M. J., Richelsen B., Bruun J. M., Li W., Hansen J. B., Pedersen S. B., J. Clinic. Endocrinol. Metabol. 2020, 105, e994. PubMed

Pan R., Zhu X., Maretich P., Chen Y., Front. Endocrinol. 2020, 11, 185. PubMed PMC

Sharma A. K., Khandelwal R., Wolfrum C., Cell Metab. 2024, 36, p1184. PubMed

Niemann B., Haufs‐Brusberg S., Puetz L., Feickert M., Jaeckstein M. Y., Hoffmann A., Zurkovic J., Heine M., Trautmann E.‐M., Müller C. E., Nature 2022, 609, 361. PubMed PMC

a) Feng J., Zhu H., Lukeš J., Korabečná M., Fohlerova Z., Mei T., Chang H., Neužil P., Sens. Actuators, A 2021, 323, 112643;

b) Zhu H., Wang L., Feng J., Neuzil P., Fundament. Res. 2023.

a) Ding H., Liu K., Zhao X., Su B., Jiang D., J. Am. Chem. Soc. 2023, 145, 22433; PubMed

b) Huang L.‐Q., Ding X.‐L., Pan X.‐T., Li Z.‐Q., Wang K., Xia X.‐H., Chem. Commun. 2023, 59, 876; PubMed

c) Haeberle W., Pantea M., Hoerber J., Ultramicroscopy 2006, 106, 678. PubMed

a) Zhang Y., Zhu W., Hui F., Lanza M., Borca‐Tasciuc T., Rojo M. Muñoz, Adv. Funct. Mater. 2020, 30, 1900892;

b) Kim K., Song B., Fernández‐Hurtado V., Lee W., Jeong W., Cui L., Thompson D., Feist J., Reid M. T. H., García‐Vidal F. J., Nature 2015, 528, 387. PubMed

Kim K., Jeong W., Lee W., Reddy P., ACS Nano 2012, 6, 4248. PubMed

Shah D. U., Konnerth J., Ramage M. H., Gusenbauer C., Sci. Rep. 2019, 9, 16667. PubMed PMC

Cui L., Hur S., Akbar Z. A., Klöckner J. C., Jeong W., Pauly F., Jang S.‐Y., Reddy P., Meyhofer E., Nature 2019, 572, 628. PubMed

Wang C., Xu R., Tian W., Jiang X., Cui Z., Wang M., Sun H., Fang K., Gu N., Cell Res. 2011, 21, 1517. PubMed PMC

Tian W., Wang C., Wang J., Chen Q., Sun J., Li C., Wang X., Gu N., Nanotechnology 2015, 26, 355501. PubMed

Roh H. H., Lee J. S., Kim D. L., Park J., Kim K., Kwon O., Park S. H., Choi Y. K., Majumdar A., J. Vac. Sci. Technol. B 2006, 24, 2405.

Nadappuram B. P., Cadinu P., Barik A., Ainscough A. J., Devine M. J., Kang M., Gonzalez‐Garcia J., Kittler J. T., Willison K. R., Vilar R., Nat. Nanotechnol. 2019, 14, 80. PubMed

Holub M., Adobes‐Vidal M., Frutiger A., Gschwend P. M., Pratsinis S. E., Momotenko D., ACS Nano 2020, 14, 7358. PubMed

Svatoš V., Gablech I., Pekárek J., Klempa J., Neužil P., Infrared Phys. Technol. 2018, 93, 286.

Zhang D., Li C., Zhang C., Slipchenko M. N., Eakins G., Cheng J.‐X., Sci. Adv. 2016, 2, e1600521. PubMed PMC

Bai Y., Zhang D., Li C., Liu C., Cheng J.‐X., J. Phys. Chem. B 2017, 121, 10249. PubMed PMC

Li Z., Aleshire K., Kuno M., Hartland G. V., J. Phys. Chem. B 2017, 121, 8838. PubMed

Samolis P. D., Sander M. Y., Opt. Express 2019, 27, 2643. PubMed

Bai Y., Zhang D., Lan L., Huang Y., Maize K., Shakouri A., Cheng J.‐X., Sci. Adv. 2019, 5, eaav7127. PubMed PMC

Li X., Zhang D., Bai Y., Wang W., Liang J., Cheng J.‐X., Anal. Chem. 2019, 91, 10750. PubMed

Donner J. S., Thompson S. A., Kreuzer M. P., Baffou G., Quidant R., Nano Lett. 2012, 12, 2107. PubMed

Wu Y., Alam M. N. A., Balasubramanian P., Ermakova A., Fischer S., Barth H., Wagner M., Raabe M., Jelezko F., Weil T., Nano Lett. 2021, 21, 3780. PubMed PMC

Meng L., Jiang S., Song M., Yan F., Zhang W., Xu B., Tian W., ACS Appl. Mater. Interfaces 2020, 12, 26842. PubMed

Kalytchuk S., Poláková K., Wang Y., Froning J. P., Cepe K., Rogach A. L., Zbořil R., ACS Nano 2017, 11, 1432. PubMed

Bu C., Mu L., Cao X., Chen M., She G., Shi W., ACS Appl. Mater. Interfaces 2018, 10, 33416. PubMed

Datta R., Heaster T. M., Sharick J. T., Gillette A. A., Skala M. C., J. Biomed. Opt. 2020, 25, 071203. PubMed PMC

Neuzil P., Cheng F., Soon J. B. W., Qian L. L., Reboud J., Lab Chip 2010, 10, 2818. PubMed

a) Gaňová M., Wang X., Yan Z., Zhang H., Lednický T., Korabečná M., Neužil P., RSC Adv. 2022, 12, 2375; PubMed PMC

b) Ni S., Zhu H., Neuzil P., Yobas L., Lab Chip 2023, 23, 1926. PubMed

Uchiyama S., Gota C., Rev. Anal. Chem. 2017, 36, 20160021.

Sugimura T., Kajimoto S., Nakabayashi T., Angew. Chem. 2020, 132, 7829. PubMed

Vieites‐Prado A., Iglesias‐Rey R., Fernández‐Susavila H., da Silva‐Candal A., Rodríguez‐Castro E., Gröhn O. H. J., Wellmann S., Sobrino T., Castillo J., Campos F., Stroke 2016, 47, 2386. PubMed

Schreurs T. J. L., Gorkum R., Zhang X. U., Faber D. J., van Leeuwen T. G., Nicolay K., Strijkers G. J., J. Biomed. Opt. 2017, 22, 036001. PubMed

West C. L., Doughty A. C. V., Liu K., Chen W. R., J. Bio‐X Res. 2019, 2, 159. PubMed PMC

Zhou Y., Li M., Liu W., Sankin G., Luo J., Zhong P., Yao J., Optica 2019, 6, 198. PubMed PMC

Zhang H., Ren M., Zhang S., Liu J., Qin H., Phys. Scr. 2023, 98, 032001.

Hong S., Dechaumphai E., Green C. R., Lal R., Murphy A. N., Metallo C. M., Chen R., Nat. Commun. 2020, 11, 2982. PubMed PMC

Hur S., Mittapally R., Yadlapalli S., Reddy P., Meyhofer E., Nat. Commun. 2020, 11, 2983. PubMed PMC

Feng J., Svatoš V., Liu X., Chang H., Neužil P., TrAC, Trends Anal. Chem. 2018, 109, 43.

Usamentiaga R., Venegas P., Guerediaga J., Vega L., Molleda J., Bulnes F. G., Sensors 2014, 14, 12305. PubMed PMC

Zhou J., Del Rosal B., Jaque D., Uchiyama S., Jin D., Nat. Methods 2020, 17, 967. PubMed

Majumdar A., Lai J., Chandrachood M., Nakabeppu O., Wu Y., Shi Z., Rev. Sci. Instrum. 1995, 66, 3584.

Kasas S., Radotic K., Longo G., Saha B., Alonso‐Sarduy L., Dietler G., Roduit C., Microsc. Res. Tech. 2013, 76, 357. PubMed