Mitochondrial respiratory complex II is altered in renal carcinoma
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
39486656
DOI
10.1016/j.bbadis.2024.167556
PII: S0925-4439(24)00550-7
Knihovny.cz E-resources
- Keywords
- Complex II, Metabolism, Mitochondria, Organoids, Renal cell carcinoma, Succinate dehydrogenase,
- MeSH
- Antigens, Neoplasm MeSH
- Adult MeSH
- Carbonic Anhydrase IX metabolism genetics MeSH
- Carcinoma, Renal Cell * pathology metabolism genetics MeSH
- Middle Aged MeSH
- Humans MeSH
- DNA, Mitochondrial genetics metabolism MeSH
- Mitochondria * metabolism pathology genetics MeSH
- Mutation MeSH
- Von Hippel-Lindau Tumor Suppressor Protein genetics metabolism MeSH
- Kidney Neoplasms * pathology metabolism genetics MeSH
- Electron Transport Complex II * metabolism genetics MeSH
- Aged MeSH
- Succinate Dehydrogenase genetics metabolism MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens, Neoplasm MeSH
- CA9 protein, human MeSH Browser
- Carbonic Anhydrase IX MeSH
- DNA, Mitochondrial MeSH
- Von Hippel-Lindau Tumor Suppressor Protein MeSH
- Electron Transport Complex II * MeSH
- respiratory complex II MeSH Browser
- SDHB protein, human MeSH Browser
- Succinate Dehydrogenase MeSH
- VHL protein, human MeSH Browser
BACKGROUND: Renal cell carcinoma (RCC) is a disease typified by anomalies in cell metabolism. The function of mitochondria, including subunits of mitochondrial respiratory complex II (CII), in particular SDHB, are often affected. Here we investigated the state and function of CII in RCC patients. METHODS: We evaluated tumour tissue as well as the adjacent healthy kidney tissue of 78 patients with RCC of different histotypes, focusing on their mitochondrial function. As clear cell RCC (ccRCC) is by far the most frequent histotype of RCC, we focused on these patients, which were grouped based on the pathological WHO/ISUP grading system to low- and high-grade patients, indicative of prognosis. We also evaluated mitochondrial function in organoids derived from tumour tissue of 7 patients. RESULTS: ccRCC tumours were characterized by mutated von Hippel-Lindau gene and high expression of carbonic anhydrase IX. We found low levels of mitochondrial DNA, protein and function, together with CII function in ccRCC tumour tissue, but not in other RCC types and non-tumour tissues. Mitochondrial content increased in high-grade tumours, while the function of CII remained low. Tumour organoids from ccRCC patients recapitulated molecular characteristics of RCC tissue. CONCLUSIONS: Our findings suggest that the state of CII, epitomized by its assembly and SDHB levels, deteriorates with the progressive severity of ccRCC. These observations hold the potential for stratification of patients with worse prognosis and may guide the exploration of targeted therapeutic interventions.
Department of Clinical and Molecular Sciences Polytechnic University of Marche 60126 Ancona Italy
Department of Molecular Endocrinology Institute of Endocrinology 110 00 Prague Czech Republic
General University Hospital 128 08 Prague Czech Republic
General University Hospital Kralovske Vinohrady 100 34 Prague Czech Republic
Institute of Biotechnology Czech Academy of Sciences 252 50 Prague West Czech Republic
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