Gradual chromosomal lagging drive programmed genome elimination in hemiclonal fishes from the genus Hypseleotris

. 2024 Nov 06 ; 14 (1) : 26866. [epub] 20241106

Jazyk angličtina Země Anglie, Velká Británie Médium electronic

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid39501046

Grantová podpora
23-0728K Grantová Agentura České Republiky
RVO 67985904 Ústav živocišné fyziologie a genetiky AV CR
RVO 67985904 Ústav živocišné fyziologie a genetiky AV CR
MSM200451701 Akademie Věd České Republiky
EXCELLENCE CZ.02.1.01/0.0/0.0/15_003/0000460 OP RDE Ministerstvo Školství, Mládeže a Tělovýchovy
DP150100608 Australian Research Council

Odkazy

PubMed 39501046
PubMed Central PMC11538498
DOI 10.1038/s41598-024-78278-6
PII: 10.1038/s41598-024-78278-6
Knihovny.cz E-zdroje

Most eukaryotes maintain the stability of their cellular genome sizes to ensure genome transmission to offspring through sexual reproduction. However, some alter their genome size by selectively eliminating parts or increasing ploidy at specific developmental stages. This phenomenon of genome elimination or whole genome duplication occurs in animal hybrids reproducing asexually. Such genome alterations occur during gonocyte development ensuring successful reproduction of these hybrids. Although multiple examples of genome alterations are known, the underlying molecular and cellular processes involved in selective genome elimination and duplication remain largely unknown. Here, we uncovered the process of selective genome elimination and genome endoreplication in hemiclonal fish hybrids from the genus Hypseleotris. Specifically, we examined parental sexual species H. bucephala and hybrid H. bucephala × H. gymnocephala (HB × HX). We observed micronuclei in the cytoplasm of gonial cells in the gonads of hybrids, but not in the parental sexual species. We also observed misaligned chromosomes during mitosis which were unable to attach to the spindle. Moreover, we found that misaligned chromosomes lag during anaphase and subsequently enclose in the micronuclei. Using whole mount immunofluorescent staining, we showed that chromatid segregation has failed in lagging chromosomes. We also performed three-dimensional comparative genomic hybridization (3D-CGH) using species-specific probes to determine the role of micronuclei in selective genome elimination. We repeatedly observed that misaligned chromosomes of the H. bucephala genome were preferentially enclosed in micronuclei of hybrids. In addition, we detected mitotic cells without a mitotic spindle as a potential cause of genome duplication. We conclude that selective genome elimination in the gonads of hybrids occurs through gradual elimination of individual chromosomes of one parental genome. Such chromosomes, unable to attach to the spindle, lag and become enclosed in micronuclei.

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