The purpose of this study was to determine the effects of resistance training (RT) alongside creatine-hydrochloride (Cr-HCl) or creatine monohydrate (CrM) supplementation on anabolic/catabolic hormones, strength, and body composition. Forty participants with an age range of 18-25 years were randomly divided into four groups (n=10): RT+Cr-HCl (0.03 g.kg-1 of body mass), RT+CrM-loading phase (CrM-LP) (0.3 g.kg-1 of body mass for five days (loading) and 0.03 g.kg-1 body mass for 51 days (maintenance)), RT+CrM-without loading phase (CrM-WLP) (0.03 g.kg-1 body mass), and RT+placebo (PL). The participants consumed supplements and performed RT with an intensity of 70-85 % 1RM for eight weeks. Before and after the training and supplementation period, strength (1RM), body composition (percent body fat (PBF), skeletal muscle mass (SMM), muscular cross-sectional area (MCSA)) and serum levels of testosterone, growth hormone (GH), insulin-like growth factor-1 (IGF-1), cortisol, adrenocorticotropic hormone (ACTH), follistatin and myostatin were measured. The results showed that in the supplementation groups, strength, arm and thigh MCSA, and SMM significantly increased, and PBF significantly decreased (P

Department of Exercise Physiology Faculty of Sport Sciences University of Guilan Rasht Iran

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Braissant O, Henry H, Beard E, Uldry J. Creatine deficiency syndromes and the importance of creatine synthesis in the brain. Amino Acids. 2011;40:1315–1324. doi: 10.1007/s00726-011-0852-z. PubMed DOI

Wyss M, Kaddurah-Daouk R. Creatine and creatinine metabolism. Physiol Rev. 2000;80:1107–1213. doi: 10.1152/physrev.2000.80.3.1107. PubMed DOI

Bagchi D, Nair S, Sen CD. Nutrition and Enhanced Sports Performance. Second Edition. Elsevier; 2019. p. 818.

Bassit RA, Curi R, Rosa LC. Creatine supplementation reduces plasma levels of pro-inflammatory cytokines and PGE 2 after a half-ironman competition. Amino Acids. 2008;35:425–431. doi: 10.1007/s00726-007-0582-4. PubMed DOI

Guimaraes-Ferreira L, Pinheiro CHJ, Gerlinger-Romero F, Vitzel KF, Nachbar RT, Curi R, Nunes MT. Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle. Eur J Appl Physiol. 2012;112:3905–3911. doi: 10.1007/s00421-012-2378-9. PubMed DOI

Kreider RB, Kalman DS, Antonio J, Ziegenfuss TN, Wildman R, Collins R, Candow DG, et al. International Society of Sports Nutrition position stand: Safety and efficacy of creatine supplementation in exercise, sport, and medicine. J Int Soc Sports Nutr. 2017;14:18. doi: 10.1186/s12970-017-0173-z. PubMed DOI PMC

Becque MD, Lochmann JD, Melrose DR. Effects of oral creatine supplementation on muscular strength and body composition. Med Sci Sports Exerc. 2000;32:654–658. doi: 10.1097/00005768-200003000-00016. PubMed DOI

Burke DG, Candow DG, Chilibeck PD, MacNeil LG, Roy BD, Tarnopolsky MA, Ziegenfuss T. Effect of creatine supplementation and resistance-exercise training on muscle insulin-like growth factor in young adults. Int J Sport Nutr. 2008;18:389. doi: 10.1123/ijsnem.18.4.389. PubMed DOI

Willoughby DS, Rosene JM. Effects of oral creatine and resistance training on myogenic regulatory factor expression. Med Sci Sports Exerc. 2003;35:923–929. doi: 10.1249/01.MSS.0000069746.05241.F0. PubMed DOI

Kreider RB, Jager R, Purpura M. Bioavailability, Efficacy, Safety, and Regulatory Status of Creatine and Related Compounds: A Critical Review. Nutrients. 2022;14:1035. doi: 10.3390/nu14051035. PubMed DOI PMC

Fazio C, Elder CL, Harris MM. Efficacy of alternative forms of creatine supplementation on improving performance and body composition in healthy subjects: A systematic review. J Strength Cond Res. 2022;36:2663–2670. doi: 10.1519/JSC.0000000000003873. PubMed DOI

Jager R, Purpura M, Shao A, Inoue T, Kreider RB. Analysis of the efficacy, safety, and regulatory status of novel forms of creatine. Amino Acids. 2011;40:1369–1383. doi: 10.1007/s00726-011-0874-6. PubMed DOI PMC

Gufford BT, Sriraghavan K, Miller NJ, Miller DW, Gu XC, Vennerstrom JL, Robinson DH. Physicochemical characterization of creatine N-methylguanidinium salts. J Diet Suppl. 2010;7:240–252. doi: 10.3109/19390211.2010.491507. PubMed DOI

Alraddadi EA, Lillico R, Vennerstrom JL, Lakowski TM, Miller DW. Absolute oral bioavailability of creatine monohydrate in rats: Debunking a myth. Pharmaceutics. 2018;10:31. doi: 10.3390/pharmaceutics10010031. PubMed DOI PMC

Dash AK, Miller DW, Huai-Yan H, Carnazzo J, Stout JR. Evaluation of creatine transport using Caco-2 monolayers as an in vitro model for intestinal absorption. J Pharm Sci. 2001;90:1593–1598. doi: 10.1002/jps.1109. PubMed DOI

Stoppani J. Supplement Breakdown: Creatine HCl. JS Stoppani Blog; Westlake Village, CA, USA: 2021. p. 24.

Kreider RB. Species-specific responses to creatine supplementation. Am J Physiol Regul Integr Comp Physiol. 2003;285:R725–R726. doi: 10.1152/ajpregu.00375.2003. PubMed DOI

Tayebi M, Arazi H. Is creatine hydrochloride better than creatine monohydrate for the improvement of physical performance and hormonal changes in young trained men? Sci Sports. 2020;35:e135–e141. doi: 10.1016/j.scispo.2019.07.013. DOI

de França E, Avelar B, Yoshioka C, Santana JO, Madureira D, Rocha LY, Zocoler CA, et al. Creatine HCl and Creatine Monohydrate Improve Strength but Only Creatine HCl Induced Changes on Body Composition in Recreational Weightlifters. Food Sci Nutr. 2015;6:1624. doi: 10.4236/fns.2015.617167. DOI

Arazi H, Rahmaninia F, Hosseini K, Asadi A. Effects of short term creatine supplementation and resistance exercises on resting hormonal and cardiovascular responses. Sci Sports. 2015;30:105–109. doi: 10.1016/j.scispo.2014.03.006. DOI

Fleck SJ, Kraemer W. Designing Resistance Training Programs. Human Kinetics. 2014:508. doi: 10.5040/9781718225107. DOI

Baechle TR, Earle RW, Wathen D. Resistance training. In: Baechle TR, Earle RW, editors. Essentials of Strength Training and Conditioning. 3rd ed. Champaign, IL: Human Kinetics; 2008. p. 401.

Negaresh R, Ranjbar R, Habibi A, Mokhtarzade M, Fokin A, Gharibvand M.The effect of resistance training on quadriceps muscle volume and some growth factors in elderly and young men Adv Gerontol 201730880–887.. PubMed

Saremi A, Gharakhanloo R, Sharghi S, Gharaati MR, Larijani B, Omidfar K. Effects of oral creatine and resistance training on serum myostatin and GASP-1. Mol Cell Endocrinol. 2010;317:25–30. doi: 10.1016/j.mce.2009.12.019. PubMed DOI

Enes A, Alves RC, Schoenfeld BJ, Oneda G, Perin SC, Trindade TB, et al. Rest-pause and drop-set training elicit similar strength and hypertrophy adaptations compared with traditional sets in resistance-trained males. Appl Physiol Nutr Metab. 2021;46:1417–1424. doi: 10.1139/apnm-2021-0278. PubMed DOI

Heymsfield SB, McManus C, Smith J, Stevens V, Nixon DW. Anthropometric measurement of muscle mass: revised equations for calculating bone-free arm muscle area. Am J Clin Nutr. 1982;36:680–690. doi: 10.1093/ajcn/36.4.680. PubMed DOI

Knapik JJ, Staab JS, Harman EA. Validity of an anthropometric estimate of thigh muscle cross-sectional area. Med Sci Sports Exerc. 1996;28:1523–1530. doi: 10.1097/00005768-199612000-00013. PubMed DOI

Tibana RA, Prestes J, da Nascimento DC, Martins OV, De Santana FS, Balsamo S. Higher muscle performance in adolescents compared with adults after a resistance training session with different rest intervals. J Strength Cond Res. 2012;26:1027–1032. doi: 10.1519/JSC.0b013e31822dfefb. PubMed DOI

Brown LE, Weir J.ASEP procedures recommendation I: Accurate assessment of muscular strength and power J Exerc Physiol Online 200141–21..

Hakkinen K, Pakarinen A, Kraemer WJ, Hakkinen A, Valkeinen H, Alen M. Selective muscle hypertrophy, changes in EMG, and force, and serum hormones during strength training in older women. J Appl Physiol. 2001;91:569–580. doi: 10.1152/jappl.2001.91.2.569. PubMed DOI

Kreider RB, Leutholtz BC, Katch FI, Katch VL. Exercise and Sport Nutrition. Santa Barbara, CA: Fitness Technologies Press; 2009.

Greenwood MB, Cooke M, Ziegenfuss TS, Kalman D, Antonio J. Nutritional Supplements in Sports and Exercise. Second Edition. Springer International Publishing; Switzerland: 2015. p. 385. DOI

Sword DO, Carolina S.Exercise as a Management Strategy for the Overweight and Obese: Where Does Resistance Exercise Fit In? Strength Cond J 20133589–89..

Lakens D. Calculating and reporting effect sizes to facilitate cumulative science: a practical primer for t-tests and ANOVAs. Front Psychol. 2013;4:863. doi: 10.3389/fpsyg.2013.00863. PubMed DOI PMC

Mielgo-Ayuso J, Zourdos M, Urdampilleta A, Calleja-Gonzalez J, Seco J, Cordova A. Relationship of long-term macronutrients intake on anabolic-catabolic hormones in female elite volleyball players. Nutr Hosp. 2017;34:1155–1162. doi: 10.20960/nh.763. PubMed DOI

Tayebi MM, Yousefpour M, Ghahari L. Effects of creatine hydrochloride supplementation on physical performance and hormonal changes in soldiers. þPhys Act Rev. 2021;9:93–99. doi: 10.16926/par.2021.09.11. DOI

Safdar A, Yardley NJ, Snow R, Melov S, Tarnopolsky MA. Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation. Physiol Genomics. 2008;32:219–228. doi: 10.1152/physiolgenomics.00157.2007. PubMed DOI

Mobley CB, Fox CD, Ferguson BS, Amin RH, Dalbo VJ, Baier S, Rathmacher JA, et al. L-leucine, beta-hydroxy-beta methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy. J Int Soc Sports Nutr. 2014;11:38. doi: 10.1186/1550-2783-11-38. PubMed DOI PMC

Schiaffino S, Dyar KA, Ciciliot S, Blaauw B, Sandri M. Mechanisms regulating skeletal muscle growth and atrophy. FEBS J. 2013;17:4294–4314. doi: 10.1111/febs.12253. PubMed DOI

Vingren JL, Kraemer WJ, Ratamess NA, Anderson JM, Volek JS, Maresh CM. Testosterone physiology in resistance exercise and training: the up-stream regulatory elements. Sports Med. 2010;40:1037–1053. doi: 10.2165/11536910-000000000-00000. PubMed DOI

Persky AM, Brazeau GA, Hochhaus G. Pharmacokinetics of the dietary supplement creatine. Clin Pharmacokinet. 2003;42:557–574. doi: 10.2165/00003088-200342060-00005. PubMed DOI

Deldicque L, Decombaz J, Zbinden Foncea H, Vuichoud J, Poortmans JR, Francaux M. Kinetics of creatine ingested as a food ingredient. Eur J Appl Physiol. 2008;102:133–143. doi: 10.1007/s00421-007-0558-9. PubMed DOI

Tuckfield C. First use of creatine hydrochloride in premanifest huntington disease. Med J Aust. 2015;202:378–381. doi: 10.5694/mja14.01070. PubMed DOI

Lanhers C, Pereira B, Naughton G, Trousselard M, Lesage FX, Dutheil F. Creatine supplementation and lower limb strength performance: a systematic review and meta-analyses. Sports Med. 2015;45:1285–1294. doi: 10.1007/s40279-015-0337-4. PubMed DOI

Lanhers C, Pereira B, Naughton G, Trousselard M, Lesage FX, Dutheil F. Creatine supplementation and upper limb strength performance: A systematic review and meta-analysis. Sports Med. 2017;47:163–173. doi: 10.1007/s40279-016-0571-4. PubMed DOI

Wu S-H, Chen K-L, Hsu C, Chen H-C, Chen J-Y, Yu S-Y, Shiu Y-J. Creatine Supplementation for Muscle Growth: A Scoping Review of Randomized Clinical Trials from 2012 to 2021. Nutrients. 2022;4:1255. doi: 10.3390/nu14061255. PubMed DOI PMC

Mills S, Candow DG, Forbes SC, Neary JP, Ormsbee MJ, Antonio J. Effects of creatine supplementation during resistance training sessions in physically active young adults. Nutrients. 2020;12:1880. doi: 10.3390/nu12061880. PubMed DOI PMC

Chilibeck PD, Kaviani M, Candow DG, Zello GA. Effect of creatine supplementation during resistance training on lean tissue mass and muscular strength in older adults: A meta-analysis. Open Access J Sports Med. 2017;8:213–226. doi: 10.2147/OAJSM.S123529. PubMed DOI PMC

Bazzucchi I, Felici F, Sacchetti M. Effect of short-term creatine supplementation on neuromuscular function. Med Sci Sports Exerc. 2009;41:1934–1941. doi: 10.1249/MSS.0b013e3181a2c05c. PubMed DOI

Schiaffino S, Mammucari C. Regulation of skeletal muscle growth by the IGF-1-akt/pKB pathway: insights from genetic models. Skeletal Muscle. 2011;1:1–14. doi: 10.1186/2044-5040-1-4. PubMed DOI PMC

Ribeiro AS, Avelar A, Kassiano W, Nunes JP, Schoenfeld BJ, Aguiar AF, Trindade MCC, et al. Creatine supplementation does not influence the ratio between intracellular water and skeletal muscle mass in resistance-trained men. Int J Sport Nutr Exerc Metab. 2020;30:405–411. doi: 10.1123/ijsnem.2020-0080. PubMed DOI

Forbes SC, Candow DG, Krentz JR, Roberts MD, Young KC. Changes in fat mass following creatine supplementation and resistance training in adults≥ 50 years of age: a meta-analysis. J Funct Morphol Kinesiol. 2019;4:62. doi: 10.3390/jfmk4030062. PubMed DOI PMC

Lee N, Kim I, Park S, Han D, Ha S, Kwon M, Kim J, et al. Creatine inhibits adipogenesis by downregulating insulin-induced activation of the phosphatidylinositol 3-kinase signaling pathway. Stem Cells Dev. 2015;24:983–994. doi: 10.1089/scd.2014.0130. PubMed DOI

Petridou A, Siopi A, Mougios V. Exercise in the management of obesity. Metabolism. 2019;92:163–169. doi: 10.1016/j.metabol.2018.10.009. PubMed DOI

Bray GA, Heisel WE, Afshin A, Jensen MD, Dietz WH, Long M, Kushner RF, et al. The Science of Obesity Management: An Endocrine Society Scientific Statement. Endocr Rev. 2018;39:79–132. doi: 10.1210/er.2017-00253. PubMed DOI PMC