A laser-plasma source emitting photons with energies in the water window spectral range has been used to reveal the radiation chemical yields of single-strand breaks in plasmid DNA as a function of ·OH radical scavenger concentration. Direct and indirect effects were investigated separately using DNA samples with various levels of hydration. We experimentally determined the value of the efficiency factor for strand cleavage in DNA caused by the reaction with ·OH radicals at 0.11, which was previously found in the theoretical studies. Additionally, the radiation chemical yield of ·OH radicals specific to the water window radiation emission of the source was determined by comparison with the gamma radiation-induced strand break yields. The ·OH radical yield determined using the plasmid DNA samples as a model was similar to the yield found using sensitive fluorescent dosimeters in previous experiments.

Institute of Optoelectronics Military University of Technology Kaliskiego 2 Str 00 908 Warsaw Poland

Institute of Physics of the Czech Academy of Sciences Na Slovance 1999 2 182 00 Prague 8 Czech Republic

Nuclear Physics Institute of the Czech Academy of Sciences Řež 130 250 68 Řež Czech Republic

Zobrazit více v PubMed

Hieda, K. DNA damage induced by vacuum and soft X-ray photons from synchrotron radiation. PubMed DOI

Hieda, K., Suzuki, K., Hirono, T., Suzuki, M. & Furusawa, Y. Single- and double-strand breaks in pBR322 DNA by vacuum-UV from 8.3 to 20.7eV. PubMed DOI

Hieda, K., Hirono, T. & Azami, A. Single- and double-strand breaks in pBR322 plasmid DNA by monochromatic X-rays on and off the K-absorption peak of phosphorus. PubMed DOI

Yokoya, A., Watanabe, R. & Hara, T. Single- and double-strand breaks in solid pBR322 DNA induced by ultrasoft X-rays at photon energies of 388, 435 and 573 eV. PubMed DOI

Prise, M. et al. Critical energies for SSB and DSB induction in plasmid DNA by low-energy photons: Action spectra for strand-break induction in plasmid DNA irradiated in vacuum. PubMed DOI

Yokoya, A., Cunniffe, S. M. T., Watanabe, R., Kobayashi, K. & O’Neill, P. Induction of DNA strand breaks, base lesions and clustered damage sites in hydrated plasmid DNA films by ultrasoft X-rays around the phosphorus K-edge. PubMed DOI

Meesungnoen, J., Jay-Gerin, J., Filali-Mouhim, A. & Mankhetkorn, S. Low-energy electron penetration range in liquid Water. PubMed DOI

Pimblott, S. M. & LaVerne, J. A. Production of low-energy electrons by ionizing radiation. DOI

Favaudon, V. PubMed

Kusumoto, T., Kitamura, H., Hojo, S., Konishi, T. & Kodaira, S. Significant changes in yields of 7-hydroxy-coumarin-3-carboxylic acid produced under FLASH radiotherapy conditions. PubMed DOI PMC

Kusumoto, T. PubMed

Blain, G. PubMed

Montay-Gruel, P. et al. Long-term neurocognitive benefits of FLASH radiotherapy driven by reduced reactive oxygen species. PubMed DOI PMC

Labarbe, R., Hotoiu, L., Barbier, J. & Favaudon, V. A physicochemical model of reaction kinetics supports peroxyl radical recombination as the main determinant of the FLASH effect. PubMed DOI

Alanazi, A., Meesungnoen, J. & Jay-Gerin, J.-P. A computer modeling study of water radiolysis at high dose rates. Relevance to FLASH radiotherapy. PubMed

Kusumoto, T. PubMed

Wolter, H. Spiegelsysteme streifenden Einfalls als abbildende Optiken für Röntgenstrahlen. DOI

Wachulak, P. et al. “Water window’’ compact, table-top laser plasma soft X-ray sources based on a gas puff target. DOI

Davídková, M. et al. A high-power laser-driven source of sub-nanosecond soft X-ray pulses for single-shot radiobiology experiments. PubMed DOI

Zielbauer, B.

Ayele, M. et al. Development and characterization of a laser-plasma soft X-ray source for contact microscopy. DOI

Vyšín, L. et al. Chemical dosimetry in the “Water Window’’: Ferric ions and hydroxyl radicals produced by intense soft X-rays. PubMed DOI

Hicks, M. & Gebicki, J. M. Rate constants for reaction of hydroxyl radicals with Tris, Tricine and Hepes buffers. DOI

Buxton, G. V., Greenstock, C. L., Helman, W. P. & Ross, A. B. Critical review of rate constants for reactions of hydrated electrons, hydrogen atoms and hydroxyl radicals (·OH/·O - in aqueous solution). DOI

Jomova, K. et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: Chronic diseases and aging. PubMed DOI PMC

Tullius, T. D. & Greenbaum, J. A. Mapping nucleic acid structure by hydroxyl radical cleavage. PubMed DOI

Halliwell, B. & Gutteridge, J. M. C.

Cheng, F.-C., Jen, J.-F. & Tsai, T.-H. Hydroxyl radical in living systems and its separation methods. PubMed DOI

Zhao, Z. Hydroxyl radical generations form the physiologically relevant Fenton-like reactions. PubMed DOI

Fleming, A. M. & Burrows, C. J. On the irrelevancy of hydroxyl radical to DNA damage from oxidative stress and implications for epigenetics. PubMed DOI PMC

Vyšín, L. et al. Dose-rate effects in breaking DNA strands by short pulses of extreme ultraviolet radiation. PubMed DOI

Henke, B., Gullikson, E. & Davis, J. X-Ray interactions: Photoabsorption, scattering, transmission, and reflection at E = 50–30,000 eV, Z = 1–92. DOI

Adjei, D. et al. DNA strand breaks induced by soft X-ray pulses from a compact laser plasma source. DOI

LaVerne, J. A. & Pimblott, S. M. Yields of hydroxyl radical and hydrated electron scavenging reactions in aqueous solutions of biological interest. PubMed DOI

Milligan, J. R., Aguilera, J. A. & Ward, J. F. Variation of single-strand break yield with scavenger concentration for plasmid DNA irradiated in aqueous solution. PubMed DOI

Klimczak, U., Ludwig, D., Mark, F., Rettberg, P. & Schulte-Frohlinde, D. Irradiation of plasmid and phage DNA in water-alcohol mixtures: Strand breaks and lethal damage as a function of scavenger concentration. PubMed DOI

LaVerne, J. A. & Pimblott, S. M. Diffusion-kinetic modelling of the cooperative effect of scavengers on the scavenged yield of the hydroxyl radical. DOI

Mark, F., Becker, U., Herak, J. N. & Schulte-Frohlinde, D. Radiolysis of DNA in aqueous solution in the presence of a scavenger: A kinetic model based on a nonhomogeneous reaction of OH radicals with DNA molecules of spherical or cylindrical shape. PubMed DOI

Espenson, J. H.

Latulippe, D. R. & Zydney, A. L. Radius of gyration of plasmid DNA isoforms from static light scattering. PubMed DOI

Prazeres, D. M. F. Prediction of diffusion coefficients of plasmids. PubMed DOI

Debije, M. G., Strickler, M. D. & Bernhard, W. A. On the efficiency of hole and electron transfer from the hydration layer to DNA: An EPR study of crystalline DNA X-irradiated at 4 K. PubMed DOI PMC

Becker, D., Adhikary, A. & Sevilla, M. D. The role of charge and spin migration in DNA radiation damage. In

Souici, M. et al. DNA strand break dependence on Tris and arginine scavenger concentrations under ultra-soft X-ray irradiation: The contribution of secondary arginine radicals. PubMed DOI

LaVerne, J. A. OH radicals and oxidizing products in the gamma radiolysis of water. PubMed DOI

Pimblott, S. M. & LaVerne, J. A. Effect of electron energy on the radiation chemistry of liquid water. PubMed DOI

Pimblott, S. M. & LaVerne, J. A. Diffusion-kinetic theories for LET effects on the radiolysis of Water. DOI

Fulford, J., Nikjoo, H., Goodhead, D. T. & O’Neill, P. Yields of SSB and DSB induced in DNA by Al K ultrasoft X-rays and PubMed DOI

Fromm, M. & Boulanouar, O. Low energy electrons and ultra-soft X-rays irradiation of plasmid DNA. DOI

Hervé du Penhoat, M.-A. et al. Lethal effect of carbon K-shell photoionizations in Chinese hamster V79 cell nuclei: Experimental method and theoretical analysis. PubMed DOI

Fayard, B. et al. Cell inactivation and double-strand breaks: The role of core ionizations, as probed by ultrasoft X-rays. PubMed DOI

Gobert, F. N. et al. Chromosome aberrations and cell inactivation induced in mammalian cells by ultrasoft X-rays: Correlation with the core ionizations in DNA. PubMed DOI

Hervé du Penhoat, M.-A. et al. Double-strand break induction and repair in V79-4 hamster cells: The role of core ionisations, as probed by ultrasoft X-rays. PubMed DOI

Fulford, J., Bonner, P., Goodhead, D. T., Hill, M. A. & O’Neill,. Experimental determination of the dependence of OH radical yield on photon energy: A comparison with theoretical simulations. DOI

Milligan, J., Aguilera, J. A., Paglinawan, R. A., Ward, J. F. & Limoli, C. L. DNA strand break yields after post-high LET irradiation incubation with endonuclease-III and evidence for hydroxyl radical clustering. PubMed DOI

Goodhead, D. T. & Nikjoo, H. Track structure analysis of ultrasoft X-rays compared to high- and low-LET radiations. PubMed DOI

Kreipl, M. S., Friedland, W. & Paretzke, H. G. Interaction of ion tracks in spatial and temporal proximity. PubMed DOI

LaVerne, J. A. & Pimblott, S. M. Scavenger and time dependences of radicals and molecular products in the electron radiolysis of water: Examination of experiments and models. DOI

Gonzalez, D. H., Kuang, X. M., Scott, J. A., Rocha, G. O. & Paulson, S. E. Terephthalate probe for hydroxyl radicals: Yield of 2-hydroxyterephthalic acid and transition metal interference. DOI