The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.

CNRS Architecture et Réactivité de l'ARN UPR 9002 Institut de Biologie Moléculaire et Cellulaire Université de Strasbourg France

CNRS CEA Institut de Biologie Structurale Université Grenoble Alpes France

CNRS Institut de Biologie Moléculaire des plantes UPR 2357 Université de Strasbourg France

Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Praha 6 Czech Republic

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