To understand growth limitations of photosynthetic microorganisms, and to investigate whether batch growth or certain photosynthesis-related parameters predict a turbidostat (continuous growth at constant biomass concentration) growth rate, five green algal species were grown in a photobioreactor in batch and turbidostat conditions and their susceptibilities to photoinhibition of photosystem II as well as several photosynthetic parameters were measured. Growth rates during batch and turbidostat modes varied independently of each other; thus, a growth rate measured in a batch cannot be used to determine the continuous growth rate. Greatly different photoinhibition susceptibilities in tested algae suggest that different amounts of energy were invested in repair. However, photoinhibition tolerance did not necessarily lead to a fast growth rate at a moderate light intensity. Nevertheless, we report an inverse relationship between photoinhibition tolerance and minimum saturating irradiance, suggesting that fast electron transfer capacity of PSII comes with the price of reduced photoinhibition tolerance.

Department of Life Technologies Molecular Plant Biology University of Turku 20014 Turku Finland

Global Change Research Institute of the Czech Academy of Sciences Bělidla 986 4a 603 00 Brno Czech Republic

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Barbera E., Grandi A., Borella L. et al.: Continuous cultivation as a method to assess the maximum specific growth rate of photosynthetic organisms. – Front. Bioeng. Biotechnol. 7: 274, 2019. https://www.frontiersin.org/articles/10.3389/fbioe.2019.00274/full PubMed DOI PMC

Barranguet C., Kromkamp J.: Estimating primary production rates from photosynthetic electron transport in estuarine microphytobenthos. – Mar. Ecol. Prog. Ser. 204: 39-52, 2000. http://www.int-res.com/abstracts/meps/v204/p39-52/

Borowitzka M.A., Vonshak A.: Scaling up microalgal cultures to commercial scale. – Eur. J. Phycol. 52: 407-418, 2017. https://www.tandfonline.com/doi/full/10.1080/09670262.2017.1365177 DOI

Campbell D.A., Serôdio J.: Photoinhibition of photosystem II in phytoplankton: processes and patterns. – In: Larkum A., Grossman A., Raven J. (ed.): Photosynthesis in Algae: Biochemical and Physiological Mechanisms. Advances in Photosynthesis and Respiration (Including Bioenergy and Related Processes). Vol. 45. Pp. 329-365. Springer, Cham: 2020. https://link.springer.com/chapter/10.1007%2F978-3-030-33397-3_13

Eilers P.H.C., Peeters J.C.H.: A model for the relationship between light intensity and the rate of photosynthesis in phytoplankton. – Ecol. Model. 42: 199-215, 1988. https://www.sciencedirect.com/science/article/pii/0304380088900579?via%3Dihub

Flynn K.J.: Going for the slow burn: why should possession of a low maximum growth rate be advantageous for microalgae? – Plant Ecol. Divers. 2: 179-189, 2009. https://www.tandfonline.com/doi/full/10.1080/17550870903207268 DOI

García-Cubero R., Kleinegris D.M.M., Barbosa M.J.: Predicting biomass and hydrocarbon productivities and colony size in continuous cultures of Botryococcus braunii showa. – Bioresource Technol. 340: 125653, 2021. PubMed

Grobbelaar J.U., Nedbal L., Tichý V.: Influence of high frequency light/dark fluctuations on photosynthetic characteristics of microalgae photoacclimated to different light intensities and implications for mass algal cultivation. – J. Appl. Phycol. 8: 335-343, 1996. https://link.springer.com/article/10.1007/BF02178576 DOI

Hindersin S., Leupold M., Kerner M., Hanelt D.: Irradiance optimization of outdoor microalgal cultures using solar tracked photobioreactors. – Bioprocess Biosyst. Eng. 36: 345-355, 2013. https://link.springer.com/article/10.1007/s00449-012-0790-5 PubMed DOI

Inskeep W.P., Bloom P.R.: Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. – Plant Physiol. 77: 483-485, 1985. https://academic.oup.com/plphys/article/77/2/483/6081863 PubMed PMC

Komenda J.: Photosystem 2 photoinactivation and repair in the Scenedesmus cells treated with herbicides DCMU and BNT and exposed to high irradiance. – Photosynthetica 35: 477-480, 1998. https://ps.ueb.cas.cz/artkey/phs-199803-0025_photosystem-2-photoinactivation-and-repair-in-the-scenedesmus-cells-treated-with-herbicides-dcmu-and-bnt-and-ex.php

Levasseur W., Taidi B., Lacombe R. et al.: Impact of seconds to minutes photoperiods on Chlorella vulgaris growth rate and chlorophyll a and b content. – Algal Res. 36: 10-16, 2018. https://www.sciencedirect.com/science/article/pii/S221192641830465X?via%3Dihub

Li G., Campbell D.A.: Rising CO2 interacts with growth light and growth rate to alter photosystem II photoinactivation of the coastal diatom Thalassiosira pseudonana. – PLoS ONE 8: e55562, 2013. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0055562 PubMed PMC

Miyata K., Noguchi K., Terashima I.: Cost and benefit of the repair of photodamaged photosystem II in spinach leaves: roles of acclimation to growth light. – Photosynth. Res. 113: 165-180, 2012. https://link.springer.com/article/10.1007%2Fs11120-012-9767-0 PubMed

Murata N., Nishiyama Y.: ATP is a driving force in the repair of photosystem II during photoinhibition. – Plant Cell Environ. 41: 285-299, 2018. https://onlinelibrary.wiley.com/doi/full/10.1111/pce.13108 PubMed DOI

Murphy C.D., Roodvoets M.S., Austen E.J. et al.: Photoinactivation of Photosystem II in Prochlorococcus and Synechococcus. – PLoS ONE 12: e0168991, 2017. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168991 PubMed PMC

Nath K., Jajoo A., Poudyal R.S. et al.: Towards a critical understanding of the photosystem II repair mechanism and its regulation during stress conditions. – FEBS Lett. 587: 3372-3381, 2013. https://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2013.09.015 PubMed DOI

Neale P.J., Melis A.: Algal photosynthetic membrane complexes and the photosynthesis-irradiance curve: a comparison of light-adaptation responses in Chlamydomonas reinhardtii (Chlorophyta). – J. Phycol. 22: 531-538, 1986. https://onlinelibrary.wiley.com/doi/10.1111/j.1529-8817.1986.tb02497.x DOI

Pätsikkä E., Kairavuo M., Šeršen F. et al.: Excess copper predisposes photosystem II to photoinhibition in vivo by outcompeting iron and causing decrease in leaf chlorophyll. – Plant Physiol. 129: 1359-1367, 2002. https://academic.oup.com/plphys/article/129/3/1359/6110498 PubMed PMC

Raven J.A.: The cost of photoinhibition. – Physiol. Plantarum 142: 87-104, 2011. https://onlinelibrary.wiley.com/doi/10.1111/j.1399-3054.2011.01465.x PubMed DOI

Rippka R., Deruelles J., Waterbury J.B. et al.: Generic assignments, strain histories and properties of pure cultures of cyanobacteria. – Microbiology 111: 1-61, 1979. https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-111-1-1 DOI

Schreiber U., Klughammer C., Kolbowski J.: Assessment of wavelength-dependent parameters of photosynthetic electron transport with a new type of multi-color PAM chlorophyll fluorometer. – Photosynth. Res. 113: 127-144, 2012. https://link.springer.com/article/10.1007/s11120-012-9758-1 PubMed DOI PMC

Serôdio J., Campbell D.A.: Photoinhibition in optically thick samples: effects of light attenuation on chlorophyll fluorescence-based parameters. – J. Theor. Biol. 513: 110580, 2021. https://www.sciencedirect.com/science/article/pii/S0022519321000023?via%3Dihub PubMed

Sipka G., Magyar M., Mezzetti A. et al.: Light-adapted charge-separated state of photosystem II: Structural and functional dynamics of the closed reaction center. – Plant Cell 33: 1286-1302, 2021. https://academic.oup.com/plcell/article/33/4/1286/6119330 PubMed PMC

Stensjö K., Vavitsas K., Tyystjärvi T.: Harnessing transcription for bioproduction in cyanobacteria. – Physiol. Plantarum 162: 148-155, 2018. https://onlinelibrary.wiley.com/doi/10.1111/ppl.12606 PubMed DOI

Su Y., Song K., Zhang P. et al.: Progress in microalgae biofuel's commercialization. – Renew. Sust. Energ. Rev. 74: 402-411, 2017. https://www.sciencedirect.com/science/article/pii/S1364032116311352

Treves H., Raanan H., Kedem I. et al.: The mechanisms whereby the green alga Chlorella ohadii, isolated from desert soil crust, exhibits unparalleled photodamage resistance. – New Phytol. 210: 1229-1243, 2016. https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.13870 PubMed DOI

Tyystjärvi E.: Photoinhibition of photosystem II. – In: Jeon K.W. (ed.): International Review of Cell and Molecular Biology. Vol. 300. Pp. 243-303. Elsevier, Amsterdam: 2013. https://www.sciencedirect.com/science/article/pii/B9780124052109000072?via%3Dihub PubMed

Valev D., Silva Santos H., Tyystjärvi E.: Stable wastewater treatment with Neochloris oleoabundans in a tubular photobioreactor. – J. Appl. Phycol. 32: 399-410, 2020. https://link.springer.com/article/10.1007/s10811-019-01890-x DOI

Virtanen O., Khorobrykh S., Tyystjärvi E.: Acclimation of Chlamydomonas reinhardtii to extremely strong light. – Photosynth. Res. 147: 91-106, 2021. https://link.springer.com/article/10.1007/s11120-020-00802-2 PubMed DOI PMC

Virtanen O., Valev D., Kruse O. et al.: Photoinhibition and continuous growth of the wild-type and a high-light tolerant strain of Chlamydomonas reinhardtii. – Photosynthetica 57: 617-626, 2019. https://ps.ueb.cas.cz/artkey/phs-201902-0035_photoinhibition-and-continuous-growth-of-the-wild-type-and-a-high-light-tolerant-strain-of-chlamydomonas-reinha.php