We compared the applicability of 3D fibrous scaffolds, produced by our patented centrifugal spinning technology, in soft tissue engineering. The scaffolds were prepared from four different biocompatible and biodegradable thermoplastics, namely, polylactide (PLA), polycaprolactone (PCL), poly(3-hydroxybutyrate) (PHB), and poly(1,4-butylene succinate) (PBS) and their blends. The combined results of SEM and BET analyses revealed an internal hierarchically organized porosity of the polymeric micro/nanofibers. Both nanoporosity and capillary effect are crucial for the water retention capacity of scaffolds designed for tissue engineering. The increased surface area provided by nanoporosity enhances water retention, while the capillary effect facilitates the movement of water and nutrients within the scaffolds. When the scaffolds were seeded with adipose-derived stem cells (ASCs), the ingrowth of these cells was the deepest in the PLA/PCL 13.5/4 (w/w) composite scaffolds. This result is consistent with the relatively large pore size in the fibrous networks, the high internal porosity, and the large specific surface area found in these scaffolds, which may therefore be best suited as a component of adipose tissue substitutes that could reduce postoperative tissue atrophy. Adipose tissue constructs produced in this way could be used in the future instead of conventional fat grafts, for example, in breast reconstruction following cancer ablation.

Czech Agrifood Research Center Drnovská 73 161 00 Prague Czech Republic

Department of Organic Technology University of Chemistry and Technology Prague Technicka 5 166 28 Prague Czech Republic

Department of Plastic Surgery 1st Faculty of Medicine Charles University Na Bulovce Hospital Budinova 67 2 180 81 Prague Czech Republic

Department of Solid State Engineering University of Chemistry and Technology Prague Technicka 5 166 28 Prague Czech Republic

Institute of Physiology of the Czech Academy of Sciences Videnska 1083 142 00 Prague Czech Republic

J E Purkyne University in Usti nad Labem Pasteurova 3544 1 400 96 Usti nad Labem Czech Republic

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