Immunostimulant effect of heat-inactivated Mycobacterium bovis in mice challenged with vector-borne pathogens
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
40188566
DOI
10.1016/j.vaccine.2025.127076
PII: S0264-410X(25)00373-1
Knihovny.cz E-zdroje
- Klíčová slova
- Borrelia burgdorferi, Mycobacterium bovis, Plasmodium berghei, Trained immunity, Vector-borne disease,
- MeSH
- adjuvancia imunologická * aplikace a dávkování MeSH
- BCG vakcína * imunologie aplikace a dávkování MeSH
- Borrelia burgdorferi imunologie MeSH
- cytokiny MeSH
- inaktivované vakcíny imunologie aplikace a dávkování MeSH
- interferon gama imunologie MeSH
- interleukin-1alfa imunologie MeSH
- játra imunologie MeSH
- lymeská nemoc * prevence a kontrola imunologie MeSH
- makrofágy imunologie MeSH
- malárie * prevence a kontrola imunologie MeSH
- Mycobacterium bovis * imunologie MeSH
- myši inbrední BALB C MeSH
- myši inbrední C3H MeSH
- myši MeSH
- Plasmodium berghei imunologie MeSH
- protilátky bakteriální krev MeSH
- slezina imunologie mikrobiologie MeSH
- TNF-alfa imunologie MeSH
- vysoká teplota MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adjuvancia imunologická * MeSH
- BCG vakcína * MeSH
- cytokiny MeSH
- inaktivované vakcíny MeSH
- interferon gama MeSH
- interleukin-1alfa MeSH
- protilátky bakteriální MeSH
- TNF-alfa MeSH
Trained immunity is defined as an enhanced state of the innate system which leads to an improved immune response against related or non-related pathogens. Bacillus Calmette-Guérin (BCG) vaccine, a live attenuated Mycobacterium bovis strain, is currently one of the main inductors of trained immunity. The objective of the present study was to evaluate the protective effects of heat-inactivated M. bovis (HIMB) against Plasmodium berghei and Borrelia burgdorferi and characterize the immunological mechanisms involved. BALB/c and C3H/HeN mice were randomly assigned in similar number to either immunized group receiving two oral doses of HIMB with a 4-week interval, or control group treated with PBS. All the BALB/c mice were intraperitoneally infected with P. berghei while the C3H/HeN mice were subcutaneously infected with B. burgdorferi. Pathogen burden was significantly reduced in both immunized groups when compared to controls. The number of macrophages significantly decreased in the liver or in the spleen of the mice that had been immunized prior to the challenge with P. berghei or B. burgdorferi, respectively. Furthermore, the immunized groups showed an apparent upregulation of IFN-γ, TNF-α and IL-1α in the liver (P. berghei challenge) or a significant increase in IL-1α producing cells in the spleen (B. burgdorferi challenge). Our findings suggest that oral immunization with heat-inactivated mycobacteria limits pathogen burden through stimulation of the innate immune response in two vector-borne diseases in mice.
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