Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study
Language English Country Great Britain, England Media electronic
Document type Journal Article
PubMed
40231554
DOI
10.1039/d5cc00971e
Knihovny.cz E-resources
- MeSH
- CRISPR-Cas Systems MeSH
- Luminescence MeSH
- CRISPR-Associated Protein 9 * metabolism chemistry MeSH
- Spectrum Analysis, Raman MeSH
- Solutions MeSH
- Stereoisomerism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- CRISPR-Associated Protein 9 * MeSH
- Solutions MeSH
Chiroptical studies of the SpyCas9 protein are extremely rare. Nondestructive methods are needed to characterize its active ribonucleoprotein form. Using Raman optical activity (ROA) and circularly polarized luminescence (CPL), we present a new approach to detect key biomolecules involved in CRISPR-Cas technology while preserving their original nucleolytic activity.
Department of Optics Palacký University Olomouc 17 listopadu 12 77146 Olomouc Czech Republic
Jagiellonian Centre for Experimental Therapeutics Bobrzynskiego 14 30 348 Krakow Poland
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