CRISPR-Associated Protein 9 [protein Cas9]
- Terms
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CRISPR-Cas9
endonukleáza Cas9
enzym Cas9
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Cas9 Endonuclease
Cas9 Enzyme
Cas9 Protein
An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9.
- DUI
- D000076987 MeSH Browser
- CUI
- M000638955
- Previous indexing
- Bacterial Proteins (2015-2018); Endonucleases (2015-2018)
- History note
- 2019
- Public note
- 2019
Allowable subheadings
- AD
- administration & dosage
- AE
- adverse effects
- AN
- analysis 1
- AI
- antagonists & inhibitors
- BI
- biosynthesis
- BL
- blood
- CF
- cerebrospinal fluid
- CS
- chemical synthesis
- CH
- chemistry
- CL
- classification
- DF
- deficiency
- DE
- drug effects
- EC
- economics
- GE
- genetics 1
- HI
- history
- IM
- immunology 1
- IP
- isolation & purification
- ME
- metabolism 3
- PK
- pharmacokinetics
- PD
- pharmacology
- PH
- physiology 1
- PO
- poisoning
- RE
- radiation effects
- ST
- standards
- SD
- supply & distribution
- TU
- therapeutic use 3
- TO
- toxicity
- UL
- ultrastructure
- UR
- urine
Cas9 endonuclease Streptococcus pyogenes Chemical MeSH Browser
Cas9 protein, Francisella novicida Chemical MeSH Browser