Parasitic protozoa such as Lankesterella and Isospora are common in avian hosts, particularly in passerines. Despite their high prevalence, the diversity of these parasites within avian populations remains poorly understood. This study aimed to assess the diversity of Lankesterella and Isospora in passerine birds, using the SSU rRNA gene to characterize and compare the genetic variation in both parasites across multiple avian host species. For Isospora, the extraintestinal blood stages and oocysts from feces were compared. Minimum spanning networks were constructed to visualize haplogroups in relation to host specificity and to reveal the identity of various developmental stages. A total of 122 sequences from eight passerine species were used to generate a haplotype network for Lankesterella, and a total of 103 sequences (64 from blood and 39 from feces) was used for Isospora. We detected a total of 36 haplotypes for Lankesterella and 33 haplotypes for Isospora. In Lankesterella, we confirmed that the sedge warbler has its own specific lineages, whereas other warbler species share lineages belonging to three haplogroups; blue, great, marsh and willow tits have their own unique groups of lineages. Isospora is less host-specific than Lankesterella in avian hosts; nevertheless, Isospora sequences from blood and feces were identical in their respective hosts. Our findings provide insights into the diversity and host specificity of blood coccidians; moreover, we molecularly characterized the developmental stages of Isospora.

Department of Parasitology Faculty of Science Charles University 128 43 Prague Czech Republic

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Box E.D. Influence of Isospora infections on patency of avian Lankesterella (Atoxoplasma, Garnham 1950) J. Parasitol. 1967;53:1140–1147. doi: 10.2307/3276669. PubMed DOI

Keckeisen C., Šujanová A., Himmel T., Matt J., Nedorost N., Chagas C.R., Weissenböck H., Harl J. Isospora and Lankesterella Parasites (Eimeriidae, Apicomplexa) of Passeriform Birds in Europe: Infection Rates, Phylogeny, and Pathogenicity. Pathogens. 2024;13:337. doi: 10.3390/pathogens13040337. PubMed DOI PMC

Chagas C.R.F., Harl J., Preikša V., Bukauskaitė D., Ilgūnas M., Weissenböck H., Valkiūnas G. Lankesterella (Apicomplexa, Lankesterellidae) blood parasites of passeriform birds: Prevalence, molecular and morphological characterization, with notes on sporozoite persistence in vivo and development in vitro. Animals. 2021;11:1451. doi: 10.3390/ani11051451. PubMed DOI PMC

Merino S., Martínez J., Martínez-de la Puente J., Criado-Fornelio Á., Tomás G., Morales J., Lobato E., García-Fraile S. Molecular characterization of the 18S rDNA gene of an avian Hepatozoon reveals that it is closely related to Lankesterella. J. Parasitol. 2006;92:1330–1335. doi: 10.1645/GE-860R.1. PubMed DOI

Biedrzycka A., Kloch A., Migalska M., Bielański W. Molecular characterization of putative Hepatozoon sp. from the sedge warbler (Acrocephalus schoenobaenus) Parasitology. 2013;140:695–698. doi: 10.1017/S0031182012002004. PubMed DOI

Martínez González F.J., Merino S., Pérez Badás E., Almazán L., Moksnes A., Barbosa A. Hemoparasites and immunological parameters in Snow Bunting (Plectrophenax nivalis) nestlings. Polar Biol. 2018;41:1855–1866. doi: 10.1007/s00300-018-2327-0. DOI

Adl S.M., Bass D., Lane C.E., Lukeš J., Schoch C.L., Smirnov A., Agatha S., Berney C., Brown M.W., Burki F., et al. Revisions to the Classification, Nomenclature, and Diversity of Eukaryotes. J. Eukaryot. Microbiol. 2018;66:4–119. doi: 10.1111/jeu.12691. PubMed DOI PMC

Labbé A. Sporozoa. Das Tierreich. Deutschen Zoologischen Gesellschaft. 5. Lieferung. R. Friedländer und Sohn Verlag; Berlin, Germany: 1899. 180p

Alvarez Calvo J.A. New species of haemococcidia in Spanish Lacertidae. Cuad. Cienc. Biol. 1975;4:207–222.

Venkatachalam A.K.S.B., Kadlecová A., Kapustová A., Kulich Fialová M., Brzoňová J., Šálek M., Svobodová M. Factors influencing a common but neglected blood parasite prevalence in breeding populations of passerines. Parasitology. 2025:1–24. doi: 10.1017/S0031182025000095. PubMed DOI

Venkatachalam A.K.S.B., Čepička I., Hrazdilová K., Svobodová M. Host specificity of passerine Lankesterella (Apicomplexa: Coccidia) Eur. J. Protistol. 2023;90:126007. doi: 10.1016/j.ejop.2023.126007. PubMed DOI

Bennett G.F., Peirce M.A. Hepatozoon parus n. sp. from the Paridae and redescription of H. atticorae (de Beaurepaire Aragão 1911) Hoare 1924 from the Hirundinidae. Can. J. Zool. 1989;67:2859–2863.

Megía-Palma R., Martínez J., Nasri I., Cuervo J.J., Martín J., Acevedo I., Belliure J., Ortega J., García-Roa R., Selmi S., et al. Phylogenetic relationships of Isospora, Lankesterella, and Caryospora species (Apicomplexa: Eimeriidae) infecting lizards. Org. Divers. Evol. 2016;16:275–288. doi: 10.1007/s13127-015-0253-3. DOI

Knight A., Ewen J.G., Brekke P., Santure A.W. The evolutionary biology, ecology and epidemiology of coccidia of passerine birds. Adv. Parasitol. 2018;99:35–60. doi: 10.1016/bs.apar.2018.01.001. PubMed DOI

Kubiski S.V., Witte C., Burchell J.A., Conradson D., Zmuda A., Barbon A.R., Vilches-Moure J.G., Felt S.A., Rideout B. A Mitochondrial gene diversity and host specificity of Isospora in passerine birds. Front. Vet. Sci. 2022;9:847030. doi: 10.3389/fvets.2022.847030. PubMed DOI PMC

Box E.D. Isospora as an Extraintestinal Parasite of Passerine Birds 1. J. Protozool. 1981;28:244–246.

Barta J.R., Schrenzel M.D., Carreno R., Rideout B.A. The genus Atoxoplasma (Garnham 1950) as a junior objective synonym of the genus Isospora (Schneider 1881) species infecting birds and resurrection of Cystoisospora (Frenkel 1977) as the correct genus for Isospora species infecting mammals. J. Parasitol. 2005;91:726–727. doi: 10.1645/GE-3341.1. PubMed DOI

Schrenzel M.D., Maalouf G.A., Gaffney P.M., Tokarz D., Keener L.L., McClure D., Griffey D.S., Bruce M.A., Rideout B. A Molecular characterization of isosporoid coccidia (Isospora and Atoxoplasma spp.) in passerine birds. J. Parasitol. 2005;91:635–647. doi: 10.1645/GE-3310. PubMed DOI

Box E.D. Life cycles of two Isospora species in the canary, Serinus canarius Linnaeus. J. Protozool. 1977;24:57–67. doi: 10.1111/j.1550-7408.1977.tb05281.x. PubMed DOI

Trefancová A., Kvičerová J. Isospora svecica sp. n. (Apicomplexa: Eimeriidae), a new species of coccidium from the white-spotted bluethroat Luscinia svecica cyanecula (Aves: Passeriformes: Muscicapidae) Parasitol. Res. 2019;118:3043–3051. doi: 10.1007/s00436-019-06397-5. PubMed DOI

Dolnik O.V. Isospora sylvianthina (Protozoa: Coccidiida), parasite of Blackcap, does not infect Reed Warbler. Zoosyst. Ross. 2002;10:240.

Levine N.D. Isospora passeris n. sp. from the house sparrow Passer domesticus, I. lacazei, and related apicomplexan Protozoa. Trans. Am. Microsc. Soc. 1982;101:66–74. doi: 10.2307/3225571. DOI

Storchová L., Hořák D. Life-history characteristics of European birds. Glob. Ecol. Biogeogr. 2018;27:400–406. doi: 10.1111/geb.12709. DOI

Fialová M., Santolíková A., Brotánková A., Brzoňová J., Svobodová M. Complete life cycle of Trypanosoma thomasbancrofti, an avian trypanosome transmitted by culicine mosquitoes. Microorganisms. 2021;9:2101. doi: 10.3390/microorganisms9102101. PubMed DOI PMC

Kvičerová J., Pakandl M., Hypša V. Phylogenetic relationships among Eimeria spp. (Apicomplexa, Eimeriidae) infecting rabbits: Evolutionary significance of biological and morphological features. Parasitology. 2008;135:443–452. PubMed

Rozas J., Ferrer-Mata A., Sánchez-DelBarrio J.C., Guirao-Rico S., Librado P., Ramos-Onsins S.E., Sánchez-Gracia A. DnaSP 6: DNA sequence polymorphism analysis of large data sets. Mol. Biol. Evol. 2017;34:3299–3302. doi: 10.1093/molbev/msx248. PubMed DOI

Leigh J.W., Bryant D., Nakagawa S. POPART: Full-feature software for haplotype network construction. Methods Ecol. Evol. 2015;6:1110–1116. doi: 10.1111/2041-210X.12410. DOI

Schumm Y.R., Wecker C., Marek C., Wassmuth M., Bentele A., Willems H., Reiner G., Quillfeldt P. Blood parasites in Passeriformes in central Germany: Prevalence and lineage diversity of Haemosporida (Haemoproteus, Plasmodium and Leucocytozoon) in six common songbirds. PeerJ. 2019;6:e6259. doi: 10.7717/peerj.6259. PubMed DOI PMC

Wong M.L., Ahmed M.A., Sulaiman WY W., Manin B.O., Leong C.S., Quan F.S., Vythilingam I. Genetic diversity of zoonotic malaria parasites from mosquito vector and vertebrate hosts. Infect. Genet. Evol. 2019;73:26–32. doi: 10.1016/j.meegid.2019.04.010. PubMed DOI

Doussang D., Sallaberry-Pincheira N., Cabanne G.S., Lijtmaer D.A., González-Acuña D., Vianna J.A. Specialist versus generalist parasites: The interactions between host diversity, environment and geographic barriers in avian malaria. Int. J. Parasitol. 2021;51:899–911. doi: 10.1016/j.ijpara.2021.04.003. PubMed DOI

Dolnik O.V., Palinauskas V., Bensch S. Individual oocysts of Isospora (Apicomplexa: Coccidia) parasites from avian feces: From photo to sequence. J. Parasitol. 2009;95:169–174. doi: 10.1645/GE-1873.1. PubMed DOI

Svobodová M. Isospora, Caryospora and Eimeria (Apicomplexa: Eimeriidae) in Passeriform Birds from Czech Republic. Acta Protozool. 1994;33:101–108.

Lopez G., Figuerola J., Soriguer R. Time of day, age and feeding habits influence coccidian oocyst shedding in wild passerines. Int. J. Parasitol. 2007;37:559–564. doi: 10.1016/j.ijpara.2006.12.014. PubMed DOI

Dolnik O.V., Dolnik V.R., Bairlein F. The effect of host foraging ecology on the prevalence and intensity of coccidian infection in wild passerine birds. Ardea. 2010;98:97–103. doi: 10.5253/078.098.0112. DOI

Svobodová M., Cibulkova M. Isospora sp. (Apicomplexa: Eimeriidae) in icterine warbler (Hippolais icterina, Passeriformes: Sylviidae): The possibility of parents to nestlings transmission. Acta Protozool. 1995;34:233.

Kadlecová A. Master’s Thesis. Charles University; Prague, Czech Republic: Sep 18, 2024. Blood Parasites of Warblers (Acrocephalus)