Anther dehiscence is the process that facilitates pollen release from mature anthers in flowering plants. Despite its crucial importance to reproduction, the underlying developmental mechanism and its integration with environmental cues remain poorly understood. Establishing noninvasive, controlled humidity treatments of Arabidopsis thaliana flowers, we show here that high humidity prevents anthers from opening. Manipulation of stomatal densities alters dehiscence dynamics, suggesting a contribution of controlled transpiration. Furthermore, analyses of subcellular markers revealed the occurrence of a developmentally prepared and environmentally triggered programmed cell death (PCD) process in specific anther tissues, epidermis and endothecium. Notably, genetic inhibition of PCD delays anther dehiscence, whereas precocious PCD induction promotes it. Our data reveal a rapid PCD execution process modulated by ambient humidity as instrumental for timely pollen release in the flowering plant Arabidopsis.

Department of Experimental Plant Biology Faculty of Science Charles University Prague 128 00 Czechia

Department of Plant Biotechnology and Bioinformatics Ghent University Ghent 9052 Belgium

VIB Center of Plant Systems Biology Ghent 9052 Belgium

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Hygrometrically controlled programmed cell death drives anther opening and pollen release