Nanostructuring and Antioxidant Activity of Nanotherapeutics Designed by Self-Assembly of Natural Lipids and Phytochemicals
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- Klíčová slova
- biodegradable nanoparticles, lyotropic liquid crystalline mesophases, phytochemical antioxidants, self-assembled biomaterials, small-angle X-ray scattering,
- MeSH
- antioxidancia * chemie farmakologie MeSH
- fytonutrienty * chemie farmakologie MeSH
- kapalné krystaly chemie MeSH
- kurkumin chemie farmakologie MeSH
- lidé MeSH
- lipidy * chemie MeSH
- nanostruktury * chemie MeSH
- poloxamer chemie MeSH
- polyethylenglykoly chemie MeSH
- ubichinon analogy a deriváty chemie farmakologie MeSH
- vitamin E chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia * MeSH
- coenzyme Q10 MeSH Prohlížeč
- fytonutrienty * MeSH
- kurkumin MeSH
- lipidy * MeSH
- poloxamer MeSH
- polyethylenglykoly MeSH
- ubichinon MeSH
- vitamin E MeSH
Lyotropic liquid crystalline nanostructures formed by self-assembly in an aqueous medium are of fundamental interest and crucial for therapeutic applications, encapsulation of nutraceuticals, tissue engineering, and diagnostics. The biomimetic lipid bilayer building blocks impart biodegradable properties and low toxicity of the created nanoassemblies. The question of synergistic or quenching effects on the resulting bioactivity arises from the coencapsulation of multiple antioxidants (e.g., vitamin E (VitE), curcumin (CU), or coenzyme Q10) in nanocarriers of mixed nonlamellar-phase lipids (e.g., amphiphilic monoglycerides or plasmalogens with long polyunsaturated fatty acid (PUFA) chains). The response to this question should favor phytochemical-based therapies against oxidative stress and inflammatory disorders using sustainable nanomedicines. Herein, we investigate the nanodispersion of multicomponent antioxidant/lipid mixtures using the copolymer Pluronic F127 and three PEGylated amphiphiles (TPGS-PEG1000, MO-PEG2000, and DSPE-PEG2000). The purpose is to establish possible relationships between the amphiphilic pharmaceutical compositions, structural stability, degradability in the biological cell culture medium, and the effects on antioxidant activity. The structures and the topologies of the phytochemical-loaded mesophases were revealed by synchrotron small-angle X-ray scattering and cryogenic transmission electron microscopy imaging. We found that encapsulated antioxidants (CU, Q10, or VitE) fine-tune the lipid bilayer properties and the nanostructure of the self-assembled systems to form lamellar (L), inverted hexagonal (HII), or cubic (Im3m) liquid crystalline phases. The results demonstrated that the composition of the nanoassemblies (lipids, dispersing agents, and antioxidants) governs the structural organization through changes in the interfacial curvature and miscibility effects. A minimal toxicity of the nanoassemblies was observed in vitro using the human neuroblastoma cell line (SH-SY5Y). The biodegradability/stability of the nanodispersions was linked with gradual dynamic changes in nanoparticle size distribution in the biological cell culture medium (DMEM). The established enhanced reactive oxygen species (ROS)-scavenging activity of the liquid crystalline nanoformulations is of interest for developing safe pharmaceutical nanosystems for multitargeted delivery of poorly soluble phytochemicals.
Institute of Rheological Functions of Food Fukuoka 811 2501 Japan
Synchrotron SOLEIL L'Orme des Merisiers Saint Aubin 91190 France
Université Paris Saclay CNRS Institut Galien Paris Saclay Orsay 91400 France
Wenzhou Institute University of Chinese Academy of Sciences Wenzhou 325001 China
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