The development of craniofacial bones and teeth relies heavily on the Wnt signaling pathway, yet the specific mechanisms and Wnt variants involved remain under continual investigation. Using publicly available single-cell sequencing data from the mouse incisor, we reveal Wnt1 expression across dental structures and investigate its role using a Col1a1-dependent Wnt1 transgenic mouse model. Inducing Wnt1 early on affects craniofacial bone without disturbing tooth development, but prolonged embryonic induction leads to postnatal mortality with osteopetrosis-like bone overgrowth and malformed teeth. While tooth formation was initially unaffected by postnatal Wnt1 induction, prolonged activation impaired tooth root formation and odontoblast differentiation, resulting in shortened roots and thinner dentin. Three-dimensional micro-computed tomography quantification reveal that both embryonic and postnatal activation of Wnt1 significantly increase neural crest-derived craniofacial bone volume, whereas mesenchymal-derived craniofacial bones are unaffected. Importantly, osteoclastogenesis is suppressed by Wnt1 in a dose-dependent manner, revealed through bulk RNA sequencing and in vitro experiments. These findings emphasize the differential effects of Wnt1 on bone development based on origin and highlight its role in modulating osteoclast activity, indicating broader implications for craniofacial development and potential therapeutic avenues.

Centre for Oral Clinical and Translational Sciences Faculty of Dentistry Oral and Craniofacial Sciences King's College London London UK

Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic

Department of Orthodontics University of Leipzig Medical Center Leipzig Germany

Department of Osteology and Biomechanics University Medical Center Hamburg Hamburg Germany

Division of Plastic and Reconstructive Surgery Department of Surgery Stanford School of Medicine Stanford University Palo Alto CA USA

Institute for Translational Immunology and Research Center for Immunotherapy University Medical Center Johannes Gutenberg University Mainz Germany

Max Planck Institute for Evolutionary Biology Plön Germany

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