BACKGROUND AND AIMS: Understanding the relationship of root traits and crop performance under varying environmental conditions facilitates the exploitation of root characteristics in breeding and variety testing to maintain crop yields under climate change. Therefore, we (1) evaluated differences in root length and surface area between ten winter wheat varieties grown at 11 sites in Europe covering a large pedoclimatic gradient, (2) quantified differences in root response to soil, climate and management conditions between varieties, and (3) evaluated variety-specific relationships of grain yield and root length and surface area under diverse environmental conditions. METHODS: At each site, we sampled the roots to 1 m soil depth after harvest and determined various root traits by scanning and image analysis. The impacts of soil, climate and management on roots and yield of the ten varieties were analysed by means of multivariate mixed models. KEY RESULTS: Root length averaged 1.4 m root piece-1, 5007 m root m-2 soil, and 5300 m root m-2 soil and root surface area 0.039 m2 root piece-1, 40 m2 root m-2 soil, and 43 m2 root m-2 soil in 0.00-0.15 m, 0.15-0.50 m, 0.50-1.00 m soil depth, respectively. The variation in both traits was 10 times higher between sites than varieties, the latter ranging by a factor of 2 within sites. Irrespective of variety, temperature was a major driver of subsoil root traits, suggesting that warmer climates promoted root growth in deeper soil layers. Other soil and climate variables affected root length and/or root surface area of individual varieties, highlighting different degrees of root plasticity. The varieties displayed distinctly different relationships between yield and root traits under varying pedoclimatic conditions, highlighting genetic differences in yield response to environmentally driven root plasticity. CONCLUSIONS: These findings suggest that breeding efforts should target flexible root-yield relationships in the subsoil to maintain crop performance under climate change.

Agroscope Agroecology and Environment Soil Quality and Soil Use 8046 Zurich Switzerland

Agroscope Plant Production Systems Cultivation Techniques and Varieties in Arable Farming 1260 Nyon Switzerland

Austrian Agency for Health and Food Safety Seed Seedling and Variety Assessment 1220 Vienna Austria

Bundessortenamt Test Management Biochemical Biophysical and Molecular Variety Testing 30627 Hannover Germany

Central Institute for Supervising and Testing in Agriculture Plant Production Section National Plant Variety Office 60300 Brno Czech Republic

ETH Zurich Institute of Agricultural Sciences 8092 Zurich Switzerland

Institute of Agrifood Research and Technology Plant Production Sustainable Field Crops 25194 Lleida Spain

Lithuanian Research Center for Agriculture and Forestry Institute of Agriculture 58344 Kėdainiai Lithuania

National Food Chain Safety Office Agricultural Genetic Resources Directorate 1024 Budapest Hungary

Norwegian Institute of Bioeconomy Research Biogeochemistry and Soil Quality 1430 Ås Norway

Technical University Munich School of Life Sciences Precision Agriculture Lab 85354 Freising Germany

Thünen Institute Institute of Climate Smart Agriculture 38116 Braunschweig Germany

Walloon Agricultural Research Centre Crop Production Unit 5030 Gembloux Belgium

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