Mettl15-Mettl17 modulates the transition from early to late pre-mitoribosome
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
40882632
DOI
10.1016/j.str.2025.08.002
PII: S0969-2126(25)00306-5
Knihovny.cz E-zdroje
- Klíčová slova
- Trypanosoma, biogenesis, cryo-EM, methyltransferase, mitochondria, mitoribosome, molecular dynamics, ribosome assembly, translation,
- MeSH
- lidé MeSH
- methyltransferasy * metabolismus chemie genetika MeSH
- metylace MeSH
- mitochondriální ribozomy * metabolismus chemie MeSH
- protozoální proteiny * chemie metabolismus genetika MeSH
- simulace molekulární dynamiky MeSH
- Trypanosoma brucei brucei * metabolismus MeSH
- vazba proteinů MeSH
- vazebná místa MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- methyltransferasy * MeSH
- protozoální proteiny * MeSH
The biogenesis of the mitoribosomal small subunit involves a dynamic network of assembly factors. Conserved methyltransferases Mettl15 and Mettl17 act on the solvent-exposed surface of rRNA. Binding of Mettl17 is associated with the early assembly stage, whereas Mettl15 is involved in the late stage. Here, we integrate structural data from Trypanosoma brucei with mammalian homologs and molecular dynamics simulations. We reveal how the interplay of Mettl15 and Mettl17 in intermediate steps links the distinct stages of small subunit assembly. The analysis suggests a model wherein Mettl17 acts as a platform for Mettl15 recruitment. Subsequent release of Mettl17 allows a conformational change of Mettl15 for substrate recognition. Upon methylation, Mettl15 adopts a loosely bound state which leads to its replacement by initiation factors, concluding the assembly. Together, our results indicate that assembly factors Mettl15 and Mettl17 cooperate to regulate the biogenesis process.
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