Giardia intestinalis is a globally distributed protist whose epidemiology appears to be more complex than previously assumed. Recent studies suggest that it frequently asymptomatically colonizes the gastrointestinal tract of both humans and animals, yet its transmission dynamics remain poorly understood-particularly in shared environments involving close human-animal contact. Adopting a One Health perspective, this study investigated the occurrence of G. intestinalis in captive non-human primates (NHPs) and their caregivers across six zoological gardens in the Czech Republic, with a particular focus on the potential for zoonotic transmission. A total of 179 fecal samples (159 from 37 NHP species and 20 from caregivers) were analyzed using qPCR. Giardia was detected in 47 % of NHPs and 30 % of caregivers. Assemblage B was the most frequently identified genotype; however, genotyping was challenging in samples with low fecal protist load, limiting the ability to accurately assess transmission pathways. These findings reveal a high prevalence of G. intestinalis in zoo environments and underscore the importance of improved genotyping tools and further research into its transmission across species boundaries in human-managed settings.

Faculty of Science University of South Bohemia 370 05 České Budějovice Czech Republic

Institute of Parasitology Biology Centre the Czech Academy of Sciences 370 05 České Budějovice Czech Republic

Institute of Vertebrate Biology Czech Academy of Sciences Květná 8 603 65 Brno Czech Republic

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Torgerson P.R., Devleesschauwer B., Praet N., Speybroeck N., Willingham A.L., Kasuga F., et al. World Health Organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases, 2010: a data synthesis. PLoS Med. 2015;12 PubMed PMC

Adam R.D. Giardia duodenalis: biology and pathogenesis. Clin. Microbiol. Rev. 2021;34 (e00024-19) PubMed PMC

Brožová K., Jirků M., Lhotská Z., Květoňová D., Kadlecová O., Stensvold C.R., et al. The opportunistic protist, Giardia intestinalis, occurs in gut-healthy humans in a high-income country. Emerg. Microb. Infect. 2023;12:2270077. PubMed PMC

Cacciò S.M., Lalle M., Svärd S.G. Host specificity in the Giardia duodenalis species complex. Infect. Genet. Evol. 2018;65:335–345. PubMed

Heyworth M.F. Giardia duodenalis genetic assemblages and hosts. Parasite. 2016;23:13. PubMed PMC

Ryan U., Zahedi A. Molecular epidemiology of giardiasis from a veterinary perspective. Adv. Parasitol. 2019;106:209–254. PubMed

Zahedi A., Field D., Ryan U. Molecular typing of Giardia duodenalis in humans in Queensland – first report of assemblage. Parasitology. 2017;144:1154–1161. PubMed

Wielinga C., Williams A., Monis P., Thompson R.C.A. Proposed taxonomic revision of Giardia duodenalis. Infect. Genet. Evol. 2023;111 PubMed

Martínez-Díaz R.A., Sansano-Maestre J., Martínez-Herrero M.D.C., Ponce-Gordo F., Gómez-Muñoz M.T. Occurrence and genetic characterization of Giardia duodenalis from captive nonhuman primates by multi-locus sequence analysis. Parasitol. Res. 2011;109:539–544. PubMed

Levecke B., Dorny P., Geurden T., Vercammen F., Vercruysse J. Gastrointestinal protozoa in non-human primates of four zoological gardens in Belgium. Vet. Parasitol. 2007;148:236–246. PubMed

Beck R., Sprong H., Bata I., Lucinger S., Pozio E., Cacciò S.M. Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia. Vet. Parasitol. 2011;175:40–46. PubMed

Fomsgaard A., Rosenstierne M.W., Rasmussen M., Wright P., Bueno G.L., Noromalala E., et al. Prevalence, infection intensity and genotyping of Giardia duodenalis in ring-tailed lemurs Lemur catta from European zoos and wild populations. J. Zoo. Aquar. Res. 2020;8:253–258.

Bryan P.E., Romero M., Sánchez M., Torres G., Gómez W., Restrepo M., et al. Urban versus rural prevalence of intestinal parasites using multi-parallel qPCR in Colombia. Am. J. Trop. Med. Hyg. 2021;104:907–909. PubMed PMC

Willenborg C.M., Červená B., Thompson P., Rosario E., Ruaux C., Vogelnest L., et al. Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling. Int. J. Parasitol. Parasit. Wildl. 2022;17:308–318. PubMed PMC

Köster P.C., Dashti A., Bailo B., Muadica A.S., Maloney J.G., Chicharro C., et al. Occurrence and genetic diversity of protist parasites in captive non-human primates, zookeepers, and free-living sympatric rats in the Córdoba Zoo Conservation Centre, Southern Spain. Animals. 2021;11:700. PubMed PMC

Marchiori E., Bono L., Voltan L., Dotto G., Tessarin C., Marcer F. Gastrointestinal parasites in non-human primates in zoological gardens in northern Italy. Animals. 2024;14:2607. PubMed PMC

Levecke B., Geldhof P., Claerebout E., Dorny P., Vercammen F., Cacciò S.M., et al. Molecular characterisation of Giardia duodenalis in captive non-human primates reveals mixed assemblage a and B infections and novel polymorphisms. Int. J. Parasitol. 2009;39:1595–1601. PubMed

Mravcová K., Štrlcová G., Mucha R., Goldová M. Zoonotic assemblages of Giardia duodenalis in captive non-human primates from the largest zoo in Slovakia. J. Parasit. Dis. 2021;45:302–305. PubMed PMC

Köster P.C., Martínez-Nevado E., González A., Abelló-Poveda M.T., Fernández-Bellon H., de la Riva-Fraga M., et al. Intestinal protists in captive non-human primates and their handlers in six European zoological gardens. Molecular evidence of zoonotic transmission. Front. Vet. Sci. 2022;8 PubMed PMC

Brynildsrud O., Tysnes K.R., Robertson L.J., Debenham J.J. Giardia duodenalis in primates: classification and host specificity based on phylogenetic analysis of sequence data. Zoonoses Public Health. 2018;65:637–647. PubMed

Hooshyar H., Rostamkhani P., Arbabi M., Delavari M. Giardia lamblia infection : review of current diagnostic strategies. Gastroenterol. A Hepatol. 2019;12:3–12. PubMed PMC

David Modrý, Pafćo B., Petrželková K., Hasegawa H. Parasites of apes an atlas of coproscopic diagnostics. Chimaira. Frankfurt Am Main. 2018:179.

Jothikumar N., Murphy J.L., Hill V.R. Detection and identification of Giardia species using real-time PCR and sequencing. J. Microbiol. Methods. 2021;189 PubMed

Capewell P., Krumrie S., Katzer F., Alexander C.L., Weir W. Parasitology molecular epidemiology of Giardia infections in the genomic era. Trends Parasitol. 2020;37:142–153. PubMed

Šejnohová A., Koutenská M., Jirků M., Brožová K., Pavlíčková Z., Kadlecová O., et al. A cross-sectional survey of Blastocystis sp. and Dientamoeba fragilis in non-human primates and their caregivers in Czech zoos. One Heal. 2024;19 PubMed PMC

Verweij J.J., Blangé R.A., Templeton K., Schinkel J., Brienen E.A.T., Van Rooyen M.A.A., et al. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J. Clin. Microbiol. 2004;42:1220–1223. PubMed PMC

Sulaiman I.M., Fayer R., Bern C., Gilman R.H., Trout J.M., Schantz P.M., et al. Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis. Emerg. Infect. Dis. 2003;9:1444–1452. PubMed PMC

BLAST: Basic Local Alignment Search Tool July 2024. https://blast.ncbi.nlm.nih.gov Available online: accessed on.

Waterhouse A.M., Procter J.B., Martin D.M.A., Clamp M., Barton G.J. Jalview version 2 - a multiple sequence alignment editor and analysis workbench. Bioinformatics. 2009;25:1189–1191. PubMed PMC

Šloufová M., Lhotská Z., Jirků M., Petrželková K.J., Stensvold C.R., Cinek O., et al. Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans. Parasite. 2022;29:30. PubMed PMC

Devaux C.A., Mediannikov O., Medkour H., Raoult D. Infectious disease risk across the growing human-non human primate interface: a review of the evidence. Front. Public Heal. 2019;7:305. PubMed PMC

Singer S.M., Fink M.Y., Angelova V.V. Recent insights into innate and adaptive immune responses to Giardia. Adv. Parasitol. 2019;106:171–208. PubMed PMC

Zhang X., Wang L., Lan X., Dan J., Ren Z., Cao S., et al. Occurrence and multilocus genotyping of Giardia duodenalis in captive non-human primates from 12 zoos in China. PloS One. 2020;15 PubMed PMC

Berrilli F., Prisco C., Friedrich K.G., Di Cerbo P., Di Cave D., De Liberato C. Giardia duodenalis assemblages and Entamoeba species infecting non-human primates in an Italian zoological garden: zoonotic potential and management traits. Parasit. Vectors. 2011;4:199. PubMed PMC

Chagas C.R.F., Gonzalez I.H.L., Salgado P.A.B., Rodrigues B., Ramos P.L. Giardia spp., ten years of parasitological data in the biggest zoo of Latin America. Ann. Parasitol. 2019;65:35–51. PubMed

Tangtrongsup S., Sripakdee D., Malaivijitnond S., Angkuratipakorn R., Lappin M. Intestinal parasites and the occurrence of zoonotic Giardia duodenalis genotype in captive gibbons at Krabokkoo wildlife breeding center, Thailand. Front. Vet. Sci. 2019;6:110. PubMed PMC

Wulandari S.A.M., Perwitasari-Farajallah D., Sulistiawati E. The gastrointestinal parasites in habituated group of Sulawesi black-crested macaque (Macaca nigra) in Tangkoko, North Sulawesi. J. Trop. Biodivers. Biotechnol. 2022;7:jtbb73044.