The opportunistic protist, Giardia intestinalis, occurs in gut-healthy humans in a high-income country
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
37815802
PubMed Central
PMC10614719
DOI
10.1080/22221751.2023.2270077
Knihovny.cz E-zdroje
- Klíčová slova
- Giardia intestinalis; human volunteers, contact with animals, conventional-PCR, qPCR, quantification,
- MeSH
- feces MeSH
- genotyp MeSH
- Giardia lamblia * genetika MeSH
- giardiáza * epidemiologie diagnóza MeSH
- lidé MeSH
- polymerázová řetězová reakce MeSH
- prevalence MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Giardia intestinalis, a cosmopolitan gastrointestinal protist, is detected mainly in patients with clinical giardiasis in high-income countries. In contrast, there is very little information on the presence of Giardia in asymptomatic individuals. Therefore, the aim of this study was to determine the presence and prevalence of Giardia in gut-healthy volunteers in the Czech Republic and to perform a comparative evaluation of different diagnostic methods, since Giardia diagnostics is complicated. Our results confirmed that the qPCR method is the most sensitive method for detecting Giardia and revealed a prevalence of 7% (22/296) in asymptomatic individuals. In most cases, the colonization intensity ranged from 10-1-101. A conventional PCR protocol targeting the TPI gene was used to identify the assemblages. However, this protocol had limited sensitivity for Giardia amplification, effectively detecting colonization above an intensity of 104. In addition, Giardia was detected in 19% of the animals, which were closely associated with the study participants. However, due to methodological limitations, zoonotic transmission could not be clearly confirmed. Notably, contact with animals proved to be the only factor that had a significant impact on the incidence of Giardia in gut-healthy humans.
Department of Bacteria Parasites and Fungi Statens Serum Institut Copenhagen Denmark
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic
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