Comparative quantification of Leishmania infantum in experimental phlebotomine sand fly infections using kDNA and single-copy Meta-1 gene qPCR assays
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články, srovnávací studie
Grantová podpora
101057690 and 10038150
European Commission
PubMed
41580822
PubMed Central
PMC12849146
DOI
10.1186/s13071-025-07231-x
PII: 10.1186/s13071-025-07231-x
Knihovny.cz E-zdroje
- Klíčová slova
- Leishmania infantum, Meta-1 gene, Parasite quantification, Sand fly vectors, kDNA, qPCR,
- MeSH
- hmyz - vektory * parazitologie MeSH
- kinetoplastová DNA * genetika MeSH
- kvantitativní polymerázová řetězová reakce * metody MeSH
- Leishmania infantum * genetika izolace a purifikace MeSH
- leishmanióza viscerální parazitologie MeSH
- Phlebotomus * parazitologie MeSH
- senzitivita a specificita MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- kinetoplastová DNA * MeSH
BACKGROUND: Leishmaniasis is a parasitic disease transmitted by female sand flies and caused by protozoan parasites of the genus Leishmania. Accurate quantification of parasite load within vectors is essential for understanding transmission dynamics and vector competence. This study compares two quantitative polymerase chain reaction (qPCR) methods for detecting and quantifying Leishmania infantum in three experimentally infected sand fly species (Phlebotomus perniciosus, Phlebotomus argentipes, and Phlebotomus orientalis). METHODS: One method targets kinetoplast minicircle DNA, which offers high sensitivity but limited quantitative precision, while the other targets the single-copy Meta-1 gene, providing more precise quantification but reduced sensitivity in low-level infections. RESULTS: A positive correlation between the two molecular markers supports a combined approach to maximize both sensitivity and accuracy in surveillance and transmission studies. Following this methodological comparison, significant differences were observed in parasite proliferation among sand fly species and L. infantum strains, with Ph. orientalis confirmed as a highly competent vector for Leishmania donovani complex. CONCLUSIONS: Together, these findings highlight that combining high-sensitivity (kinetoplast DNA [kDNA]) and single-copy (Meta-1) targets enables both accurate and sensitive quantification of Leishmania infections in sand flies, improving the assessment of parasite-vector interactions.
Department of Parasitology Faculty of Science Charles University Prague Czech Republic
Faculté de Pharmacie Université de Reims Champagne Ardenne UR ESCAPE USC ANSES PETARD Reims France
Laboratoire de Parasitologie Mycologie Centre Hospitalier Universitaire de Rennes Rennes France
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