The aim of this study was to develop multifunctional magnetic poly(ε-caprolactone) (PCL) mats with antibacterial properties for bone tissue engineering and osteosarcoma prevention. To provide good dispersion of magnetic iron oxide nanoparticles (IONs), they were first grafted with PCL using a novel three-step approach. Then, a series of PCL-based mats containing a fixed amount of ION@PCL particles and an increasing content of ascorbic acid (AA) was prepared by electrospinning. AA is known for increasing osteoblast activity and suppressing osteosarcoma cells. Composites were characterized in terms of morphology, mechanical properties, hydrolytic stability, antibacterial performance, and biocompatibility. AA affected both the fiber diameter and the mechanical properties of the nanocomposites. All produced mats were nontoxic to rat bone marrow-derived mesenchymal cells; however, a composite with 5 wt.% of AA suppressed the initial proliferation of SAOS-2 osteoblast-like cells. Moreover, AA improved antibacterial properties against Staphylococcus aureus and Escherichia coli compared to PCL. Overall, these magnetic composites, reported for the very first time, can be used as scaffolds for both tissue regeneration and osteosarcoma prevention.
- MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- Escherichia coli drug effects MeSH
- Bone and Bones MeSH
- Rats MeSH
- Ascorbic Acid * chemistry pharmacology MeSH
- Humans MeSH
- Magnetite Nanoparticles chemistry MeSH
- Cell Line, Tumor MeSH
- Nanocomposites chemistry MeSH
- Osteoblasts metabolism cytology MeSH
- Osteosarcoma pathology MeSH
- Polyesters * chemistry MeSH
- Staphylococcus aureus * drug effects growth & development MeSH
- Materials Testing MeSH
- Tissue Engineering * MeSH
- Tissue Scaffolds chemistry MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
