- Publikační typ
- abstrakt z konference MeSH
In order to re-evaluate the presence and relative quantity of 2b and 2x/d myosin heavy chain (MyHC) transcripts in rat slow soleus muscle by using real time RT-PCR we have compared the available relevant cDNA sequences and designed a new set of primers having similar melting temperatures, matching separate MyHC exons in the regions of maximal differences in MyHC coding sequences, and containing G or C at the 3´-end. These also yielded PCR products of corresponding length, which is an important requirement for real time RT-PCR quantification. The experiments were performed on 8-month-old inbred female Lewis strain rats used in our current study of regenerating transplanted muscles. The real time RT-PCR measurement confirmed the expression of all four MyHC mRNAs (type 1, 2a, 2x/d and 2b) in both fast extensor digitorum longus and slow soleus muscles, although in the soleus muscle of adult rats, only type 1 and 2a protein isoforms can be usually detected.
- Klíčová slova
- Rat slow soleus muscle, Rat fast EDL muscle, Myosin heavy chain isoforms, Real time RT-PCR,
- MeSH
- DNA primery MeSH
- financování organizované MeSH
- kosterní svaly chemie MeSH
- krysa rodu rattus MeSH
- messenger RNA analýza MeSH
- polymerázová řetězová reakce MeSH
- potkani inbrední LEW MeSH
- protein - isoformy MeSH
- svalová vlákna typu I chemie MeSH
- svalová vlákna typu II chemie MeSH
- těžké řetězce myosinu genetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- abstrakt z konference MeSH
