Genotoxic compounds, as common contaminants of the air environment, are of interest in air pollution monitoring. There are several methods to determine the level of these contaminants in different localities, many of which may be difficult to access with the use of conventional active and passive samplers. In the present study, the needles Pinus mugo Turra and Picea abies were used to monitor sampling localities in Austria, Slovakia, and the Czech Republic. Needles were extracted and chemical analysis and the genotoxicity bioassay SOS chromotest were used to obtain complex information about the chemical mixture of pollutants present and their genotoxic effects. The SOS chromotest method was optimized by using a CPRG chromogenic substrate to reduce the false positive genotoxic effect of needle extracts. Pinus mugo Turra and Picea abies were identified as suitable passive sampling matrices for long-term air monitoring using the same plants sampled at the same time. The presented study brings an innovative method for the fast screening and identification of localities loaded by genotoxic active air contaminants.
- MeSH
- borovice chemie genetika MeSH
- látky znečišťující vzduch analýza MeSH
- lidé MeSH
- monitorování životního prostředí metody MeSH
- poškození DNA genetika MeSH
- smrk chemie genetika MeSH
- testy genotoxicity metody MeSH
- znečištění ovzduší analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Rakousko MeSH
- Slovenská republika MeSH
Coniferous needles can be used as a passive air sampler of semivolatile organic compounds (SVOC) and an indicator of atmospheric pollution patterns and trends. There is limited information on whether different parts of the plant (e.g., foliage leaves, dwarf shoots, twig, etc.) contain different levels of SVOC. Only few studies have compared levels of SVOC surface layer of wax and to their total content in all needle tissues and what affects an uptake and distribution of SVOC. It is important to have better understanding of the extent to which sampling and sample preparation procedures affect measured levels of SVOC in pine needles and reduce comparability of data from samples processed in different ways. In the present study, we assessed an impact of various sampling and sample preparation techniques on the levels of SVOC in Pinus sylvestris needles. While the impact of various storing, washing and drying methods was not significant, presence of dwarf shoots in the sample or structural damage of needles affected the results significantly. Results show that levels of SVOC in dwarf shoots are 2-8 times higher than those in foliage leaves. Therefore, dwarf shoots must be carefully removed before foliage leaves extraction to improve comparability of results. There were different patterns in SVOC on the surface of the foliage leaves compared to the whole leaves. An uptake of these substances by the surface wax as well as their occurrence in the internal structure was of function of the physico-chemical properties of the substances (log Kow, molar weight).
