Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis have been cloned and sequenced, revealing 95% identity. Fluorescence in-situ hybridization (FISH) combined with tyramide amplification discriminates both genes; Mdh2a was localized into chromosome q3 and Mdh2b into chromosome q8. One kb cDNA probes detect both genes with 85% accuracy. The remaining signals were on the paralogous counterpart. Introns interrupt coding sequences at the same nucleotide as defined for mouse. Restriction polymorphism has been detected in the first intron of Mdh2a, while the individual variability in intron 6 of Mdh2b gene is represented by an insertion of incomplete retrotransposon L1Xl. Rates of nucleotide substitutions indicate that both genes are under similar evolutionary constraints. X. laevis Mdh2 genes can be used as markers for physical mapping and linkage analysis.
- MeSH
- chromozomy MeSH
- duplicitní geny MeSH
- exprimované sekvenční adresy MeSH
- financování organizované MeSH
- genetická variace MeSH
- hybridizace in situ fluorescenční MeSH
- introny MeSH
- karyotypizace MeSH
- klonování DNA MeSH
- konzervovaná sekvence MeSH
- malátdehydrogenasa genetika chemie metabolismus MeSH
- mapování chromozomů MeSH
- mitochondrie enzymologie MeSH
- molekulární sekvence - údaje MeSH
- polymorfismus genetický MeSH
- retroelementy MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie aminokyselin MeSH
- techniky amplifikace nukleových kyselin MeSH
- Xenopus laevis MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
