An efficient and facile synthesis of a large series of diverse 6-(N-substituted aminomethyl)-, 6-(O-substituted hydroxymethyl)- and 6-(S-substituted sulfanylmethyl)purine nucleosides (55 examples of both ribo- and 2'-deoxyribonucleosides), aimed at identifying novel homologues of natural nucleosides, was developed. The key transformation involved nucleophilic substitutions of Tol-protected 6-(mesyloxymethyl)purine nucleosides by primary or secondary amines, alcoholates or thiolates. While the 2'-deoxyribonucleosides were inactive, the ribonucleosides exerted considerable cytostatic effects and some anti-HCV activity with low selectivity.
- MeSH
- aminace MeSH
- antivirové látky farmakologie chemická syntéza chemie MeSH
- cytostatické látky farmakologie chemická syntéza chemie MeSH
- financování organizované MeSH
- Hepacivirus účinky léků MeSH
- hydroxylace MeSH
- lidé MeSH
- methansulfonáty chemie MeSH
- metylace MeSH
- molekulární struktura MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- purinové nukleosidy farmakologie chemická syntéza chemie MeSH
- sloučeniny síry farmakologie chemická syntéza chemie MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
A series of purine l-ribonucleosides 2a-2i bearing diverse C-substituents (alkyl, aryl, hetaryl or hydroxymethyl) in the position 6 were prepared by Pd-catalyzed cross-coupling reactions of 6-chloro-9-(2,3,5-tri-O-acetyl-beta-l-ribofuranosyl)purine with the corresponding organometallics followed by deprotection. Unlike their d-ribonucleoside enantiomers that possess strong cytostatic and anti-HCV activity, the l-ribonucleosides were inactive except for 6-benzylpurine nucleoside 2h showing moderate anti-HCV effect in replicon assay. A triphosphate of 2h did not inhibit HCV RNA polymerase.
- MeSH
- antitumorózní látky farmakologie chemická syntéza chemie MeSH
- antivirové látky farmakologie chemická syntéza chemie MeSH
- financování organizované MeSH
- HeLa buňky MeSH
- Hepacivirus účinky léků MeSH
- HL-60 buňky MeSH
- léky antitumorózní - screeningové testy MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- molekulární struktura MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- proliferace buněk účinky léků MeSH
- purinové nukleosidy farmakologie chemická syntéza chemie MeSH
- ribonukleosidy farmakologie chemická syntéza chemie MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH