Double-stranded RNA and total RNA purified from sour cherry leaves (Prunus cerasus, cv. Amarelka Chvalkovicka) was analyzed by high-throughput sequencing. BLAST annotation identified contigs with homology to several already known cherry-infecting viruses (prune dwarf virus, prunus necrotic ringspot virus, prunus virus F, little cherry virus 1) as well as contigs with sequences more distantly related to those of members of the family Betaflexiviridae and in particular to prunus virus T of the genus Tepovirus. The full genome sequence of a putative virus (6,847 nucleotides [nt]; GenBank no. MT090966) was assembled and completed at the genome ends. The genome has a typical tepovirus organization, containing three overlapping open reading frames (ORFs), encoding a replication-associated protein, a movement protein and a capsid protein, respectively. Both its genome organization and its phylogenetic relationships show that the virus belongs to the genus Tepovirus, but considering the species demarcation criteria for the family Betaflexiviridae, it appears to represent a novel virus species, and we propose the name "cherry virus T" (ChVT) for this virus.
- MeSH
- Flexiviridae klasifikace genetika izolace a purifikace MeSH
- fylogeneze MeSH
- genom virový * MeSH
- nemoci rostlin virologie MeSH
- otevřené čtecí rámce MeSH
- Prunus avium virologie MeSH
- sekvence nukleotidů MeSH
- sekvenování celého genomu MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- bodová mutace MeSH
- DNA fingerprinting MeSH
- DNA virů chemie izolace a purifikace MeSH
- epitopy genetika imunologie MeSH
- financování organizované MeSH
- fylogeneze MeSH
- komplementární DNA chemie izolace a purifikace MeSH
- molekulární evoluce MeSH
- molekulární sekvence - údaje MeSH
- monoklonální protilátky imunologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- protilátky virové imunologie MeSH
- RNA virová genetika izolace a purifikace MeSH
- sérotypizace MeSH
- slivoň virologie MeSH
- virus šarky švestky genetika imunologie izolace a purifikace MeSH
- Geografické názvy
- Turecko MeSH
