Sulfhydryl oxidase Erv1 is a ubiquitous and conserved protein of the mitochondrial intermembrane space that plays a role in the transport of small sulfur-containing proteins. In higher eukaryotes, Erv1 interacts with the mitochondrial import protein Mia40. However, Trypanosoma brucei lacks an obvious Mia40 homologue in its genome. Here we show by tandem affinity purification and mass spectrometry that in this excavate protist, Erv1 functions without a Mia40 homologue and most likely any other interaction partner. Down-regulation of TbErv1 caused a reduction of the mitochondrial membrane potential already within 24h to less than 50% when compared with control cells. The depletion of TbErv1 was accompanied by accumulation of trCOIV precursor, with a concomitant reduction of aconitase activity both in the cytosol and mitochondrion. Overall, TbErv1 seems to have a role in the mitochondrial translocation and Fe-S cluster assembly in the organelle.
- MeSH
- hmotnostní spektrometrie MeSH
- mapování interakce mezi proteiny MeSH
- mitochondrie enzymologie metabolismus MeSH
- oxidoreduktasy metabolismus MeSH
- proteiny obsahující železo a síru metabolismus MeSH
- protozoální proteiny metabolismus MeSH
- Trypanosoma brucei brucei enzymologie metabolismus MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
